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Remineralizing potential of CPP-ACP applied for 1 min in vitro

  • Original Scientific Article
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European Archives of Paediatric Dentistry Aims and scope Submit manuscript

Abstract

Aim

To evaluate (1) the remineralizing potential of Tooth Mousse® (TM) on artificial carious lesions, when applied for 1 min, and (2) the benefit of a fluoridated dentifrice prior to TM application.

Methods

Demineralisation caries-like lesions 120–200 μm deep were produced by placing molars in demineralizing solution for 96 h, sections 100–150 μm thick were then randomly assigned to four groups. Specimens were treated thrice daily with a non-fluoridated (Group A), or fluoridated dentifrice (1000 ppm) (Group B), or TM (Group C), or a fluoridated dentifrice followed by TM (Group D) then subjected to a 10-day pH cycling model. Lesion evaluation involved polarizing light microscopy and microradiography.

Results

Application of TM in isolation (Group C) for 1 min resulted in significant increase in lesion depth (LD), however, an increase in mineral content deposition (V max) was not significant. Post treatment LD increases were greatest in Group A, then Group C and Group B respectively. A decrease in LD (by 19.9 %) was noted only after fluoridated dentifrice and TM (Group D) were applied consecutively for 1 min each (p < 0.05). Mineral content deposition was also greatest in this group (Group D) and both V max and LD changes were statistically significant when compared to other groups.

Conclusions

TM alone failed to remineralize carious lesions when applied for 1 min. However, remineralization occurred after a prior application of a fluoridated dentifrice.

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Acknowledgments

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Authors and Affiliations

  1. Paediatric Dentistry, School of Dentistry, University of Western Australia, 17 Monash Avenue, Nedlands, WA, 6009, Australia

    V. Gopalakrishnan, R. P. Anthonappa & N. M. King

  2. Paediatric Dentistry, School of Dentistry and Oral Health, Griffith University, Gold Coast, Queensland, Australia

    A. Itthagarun

Authors
  1. V. Gopalakrishnan
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  2. R. P. Anthonappa
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  3. N. M. King
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  4. A. Itthagarun
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Correspondence to R. P. Anthonappa.

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Author Gopalkrishnan declares that she has no conflict of interest. Author Anthonappa declares that he has no conflict of interest. Author King declares that he has no conflict of interest. Author Itthagarun declares that she has no conflict of interest.

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Gopalakrishnan, V., Anthonappa, R.P., King, N.M. et al. Remineralizing potential of CPP-ACP applied for 1 min in vitro. Eur Arch Paediatr Dent 17, 231–237 (2016). https://doi.org/10.1007/s40368-016-0232-1

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  • DOI: https://doi.org/10.1007/s40368-016-0232-1

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