Skip to main content
SpringerLink
Log in

Turnip leaf curl disease associated with two begomoviruses in south-eastern Iran

  • Short Communication
  • Published:
Tropical Plant Pathology Aims and scope Submit manuscript

Abstract

A handful of geminiviruses have been associated with turnip leaf curl disease. As part of our turnip surveys in Kerman province (south-eastern Iran), turnip samples showing mild leaf curl disease were collected and screened for turncurto- and begomoviruses by PCR. In four out of seven samples, we identified co-infection with begomoviruses of the species Tomato yellow leaf curl virus and Watermelon chlorotic stunt virus. Neither of these two begomovirus species have been described to be associated with turnip leaf curl disease in Iran. Using infectious clones and agroinoculation of turnip, tomato and watermelon seedlings, our results indicated that single and/or co-agroinoculation of plants with the two begomovirus constructs result in symptoms with different severity in turnip, tomato and watermelon.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Aguilar I, Sánchez F, Ponz F (2000) Different forms of interference between two tobamoviruses in two different hosts. Plant Pathology 49:659–665

    Article  Google Scholar 

  • Ala-Poikela M, Svensson E, Rojas A, Horko T, Paulin L, Valkonen JPT, Kvarnheden A (2005) Genetic diversity and mixed infections of begomoviruses infecting tomato, pepper and cucurbit crops in Nicaragua. Plant Pathology 54:448–459

    Article  CAS  Google Scholar 

  • Alves-Júnior M, Alfenas-Zerbini P, Andrade EC, Esposito DA, Silva FN, da Cruz ACF, Ventrella MC, Otoni WC, Zerbini FM (2009) Synergism and negative interference during co-infection of tomato and Nicotiana benthamiana with two bipartite begomoviruses. Virology 387:257–266

  • Böttcher B, Unseld S, Ceulemans H, Russell RB, Jeske H (2004) Geminate structures of African cassava mosaic virus. Journal of Virology 78:6758–6765

    Article  PubMed  PubMed Central  Google Scholar 

  • Boulton M (2008) Construction of infectious clones for DNA viruses: mastreviruses. In: Foster GD, Johansen IE, Hong Y, Nagy PD (eds) Methods in molecular biology - plant virology protocols: from viral sequence to protein function. Humana Press, Totowa, pp 503–523

    Chapter  Google Scholar 

  • Briddon RW, Heydarnejad J, Khosrowfar F, Massumi H, Martin D, Varsani A (2010) Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran. Virus Research 152:169–175

    Article  CAS  PubMed  Google Scholar 

  • Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Archives of Virology 160:1593–1619

    Article  CAS  PubMed  Google Scholar 

  • Deng D, McGrath PF, Robinson DJ, Harrison BD (1994) Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by polymerase chain reaction with degenerate primers. Annals of Applied Biology 125:327–336

    Article  CAS  Google Scholar 

  • Esmaeili M, Heydarnejad J, Massumi M, Varsani A (2015) Analysis of Watermelon chlorotic stunt virus and Tomato leaf curl Palampur virus mixed and pseudo-recombination infections. Virus Genes 51:408–416

    Article  CAS  PubMed  Google Scholar 

  • Fazeli R, Heydarnejad J, Massumi H, Shaabanian M, Varsani A (2009) Genetic diversity and distribution of tomato-infecting begomoviruses in Iran. Virus Genes 38:311–319

    Article  CAS  PubMed  Google Scholar 

  • Grimsley N, Hohn B, Hohn T, Walden R (1986) Agroinfection, an alternative route for viral-infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences, USA 83:3282–3286

  • Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology 42:819–832

    Article  CAS  PubMed  Google Scholar 

  • Inoue-Nagata AK, Albuquerque LC, Rocha WB, Nagata TA (2004) A simple method for cloning the complete begomovirus genome using the bacteriophage phi29 DNA polymerase. Journal of Virological Methods 116:209–211

    Article  CAS  PubMed  Google Scholar 

  • Jaiswal N, Saritha RK, Datta D, Singh M, Dubey RS, Rai M, Rai AB (2012) Mixed infections of begomoviruses in pumpkins with yellow vein mosaic disease in north India. Archives of Phytopathology and Plant Protection 45:938–941

    Article  Google Scholar 

  • Kamali M, Heydarnejad J, Massumi H, Kvarnheden A, Kraberger S, Varzani A (2016) Molecular diversity of turncurtoviruses in Iran. Archives of Virology 161:551–561

    Article  CAS  PubMed  Google Scholar 

  • Kheyr-Pour A, Bananej K, Dafalla G, Caciagli P, Noris E, Ahoonmanesh A, Lecoq H, Gronenborn B (2000) Watermelon chlorotic stunt virus from the Sudan and Iran: sequence comparisons and identification of a whitefly-transmission determinant. Phytopathology 90:629–635

    Article  CAS  PubMed  Google Scholar 

  • Leke WN, Kvarnheden A (2014) Mixed infection by two west African tomato-infecting begomoviruses and ageratum leaf curl Cameroon betasatellite in tomato in Cameroon. Archives of Virology 159:3145–3148

    Article  CAS  PubMed  Google Scholar 

  • Méndez-Lozano J, Torres-Pacheco I, Fauquet CM, Rivera-Bustamante RF (2003) Interactions between geminiviruses in a naturally occurring mixture: Pepper huasteco virus and Pepper golden mosaic virus. Phytopathology 93:270–277

    Article  PubMed  Google Scholar 

  • Razavinejad S, Heydarnejad J, Kamali M, Massumi H, Kraberger S, Varsani A (2013) Genetic diversity and host range studies of turnip curly top virus. Virus Genes 46:345–353

    Article  CAS  PubMed  Google Scholar 

  • Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease 77:340–347

    Article  CAS  Google Scholar 

  • Stewart C, Kon T, Rojas M, Graham A, Martin DP, Gilbertson R, Roye M (2014) Mixed infection of Sida jamaicensis in Jamaica reveals the presence of three recombinant begomovirus DNA-A components. Archives of Virology 159:2509–2512

  • Tahir M, Amin I, Haider MS, Mansoor S, Briddon RW (2015) Ageratum enation virus, a begomovirus of weeds with the potential to infect crops. Viruses 7:647–665

  • Varma A, Malathi VG (2003) Emerging geminivirus problems: a serious threat to crop production. Annals of Applied Biology 142:145–164

    Article  CAS  Google Scholar 

  • Varsani A, Roumagnac P, Fuchs M, Navas-Castillo J, Moriones E, Idris A, Briddon RW, Rivera-Bustamante R, Zerbini FM, Martin DP (2017) Capulavirus and Grablovirus: two new genera in the family Geminiviridae. Archives of Virology. 162:1819–1831

  • Zerbini FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J, Rivera-Bustamante R, Rougmanac P, Varsani A, Report Consortium ICTV (2017) ICTV virus taxonomy profile: Geminiviridae. Journal of General Virology 98:131–133

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang YP, Uyemoto JK, Kirkpatrick BC (1998) A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods 71:45–50

    Article  CAS  PubMed  Google Scholar 

  • Zhang W, Olson NH, Baker TS, Faulkner L, Agbandje-McKenna M, Boulton MI, Davies JW, McKenna R (2001) Structure of the Maize streak virus geminate particle. Virology 279:471–477

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research work was supported by the Iran National Science Foundation (INSF) (Grant No. 92012168).

Author information

Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, 7616914111, Iran

    Jahangir Heydarnejad, Mehdi Kamali, Vahid Hassanvand, Hossain Massumi & Marzieh Shamshiri

  2. The Biodesign Center of Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and Medicine, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ, 85287-5001, USA

    Arvind Varsani

  3. Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa

    Arvind Varsani

Authors
  1. Jahangir Heydarnejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mehdi Kamali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vahid Hassanvand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hossain Massumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marzieh Shamshiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Arvind Varsani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jahangir Heydarnejad.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Section Editor: Jorge Rezende

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Heydarnejad, J., Kamali, M., Hassanvand, V. et al. Turnip leaf curl disease associated with two begomoviruses in south-eastern Iran. Trop. plant pathol. 43, 165–169 (2018). https://doi.org/10.1007/s40858-017-0196-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40858-017-0196-7

Keywords

Search

Navigation