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Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies

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Abstract

The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2–3 μg ml−1 for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 μg ml−1.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahshi-ku, Fukuoka, 812-8582, Japan

    Hiroyuki Tanaka, Shayu Yan & Yukihiro Shoyama

  2. Advanced Science and Technology Center for Cooperative Research, Kasuga, Fukuoka, 816-8580, Japan

    Norio Miura

Authors
  1. Hiroyuki Tanaka
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  2. Shayu Yan
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  3. Norio Miura
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  4. Yukihiro Shoyama
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Correspondence to Yukihiro Shoyama.

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Tanaka, H., Yan, S., Miura, N. et al. Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies. Cytotechnology 42, 101–107 (2003). https://doi.org/10.1023/B:CYTO.0000009919.62901.27

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  • DOI: https://doi.org/10.1023/B:CYTO.0000009919.62901.27

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