A preliminary information about continuous fermentation using cell recycling for improving microbial xylitol production rates

Abstract

Xylitolis a sugar-alcohol with important technological properties, such as anticariogenicity, low caloric value, and negative dissolution heat. It can be used successfully in food for mulations and pharmaceutical industries. Its production is therefore in great demand. Biotechnological xylitol production has several economic advantages in comparison with the conventional process based on the chemical reduction of xylose. The efficiency and the productivity of this fermentation chiefly depends on the microorganism and the process conditions employed. In this article a simple continuous culture with cell recycling was evaluated to enhance the capability of Candida guilliermondii FTI 20037 to produce xylitol. The fermentation was initiated batchwise by directly inoculating the grown seed culturein a 2-L bench-scale fermentor. Continuous feeding was begun at a dilution rate (D) of 0.060/h after the xylose concentration had completely consumed and the cell concentration was a bout 4.0 g/L. At a dilution rate of 0.060/h the xylitol concentration was about 15g/L and in creased by about 35%, whereas the dilution rate decreased by about 58%. Furthermore, the volumetric productivity, Q_p, markedly depended on the dilution rate, diminishing by about 37% as D was changed from 0.060 to 0.025/h. These preliminary results show us that the continous fermentation with cell recycling is a good way to study the xylitol production by xylose-fermenting yeasts.