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Cyclic AMP response element binding protein (CREB) and CREB binding protein (CBP) in global cerebral ischemia

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Abstract

Cyclic AMP (cAMP) response element binding protein (CREB) is a transcription factor that has been implicated in neuronal responses to ischemia. We examined the effect of global cerebral ischemia in the rat on the expression of CREB, its transcriptionally active phosphorylated form (pCREB), and the nuclear adaptor protein, CREB binding protein (CBP). Global ischemia induced the expression of pCREB and CBP in vulnerable neurons of the hippocampal CA1 sector. In primary cultures of murine cortical neurons subjected to hypoxia, CBP was selectively expressed in cells with morphologically intact cell nuclei, and not in cells with condensed or fragmented nuclei indicative of irreversibly damaged neurons. These results support a role for transcriptional activation by CREB and CBP in neuronal cell-survival programs following cerebral ischemia.

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Authors and Affiliations

  1. Buck Institute, 8001 Redwood Blvd., 94945, Novato, CA

    Kunlin Jin, Xiao Ou Mao & David A. Greenberg

  2. Robert S. Dow Neurobiology Laboratory, Legacy Research, 1225 N.E. 2nd Ave., 97232, Portland, OR

    Roger P. Simon

Authors
  1. Kunlin Jin
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  2. Xiao Ou Mao
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  3. Roger P. Simon
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  4. David A. Greenberg
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Correspondence to David A. Greenberg.

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Jin, K., Mao, X.O., Simon, R.P. et al. Cyclic AMP response element binding protein (CREB) and CREB binding protein (CBP) in global cerebral ischemia. J Mol Neurosci 16, 49–56 (2001). https://doi.org/10.1385/JMN:16:1:49

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  • DOI: https://doi.org/10.1385/JMN:16:1:49

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