Overview
- Editors:
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Kary B. Mullis
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La Jolla, USA
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François Ferré
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The Immune Response Corporation, Carlsbad, USA
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Richard A. Gibbs
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Institute for Molecular Genetics, Baylor College of Medicine, Houston, USA
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Table of contents (35 chapters)
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Front Matter
Pages i-xxii
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Methodology
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Basic Methodology
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- Jeffrey S. Chamberlain, Joel R. Chamberlain
Pages 38-46
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- Janet Embretson, Katherine Staskus, Ernest Retzel, Ashley T. Haase, Peter Bitterman
Pages 55-64
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Quantitation
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- F. Ferré, A. Marchese, P. Pezzoli, S. Griffin, E. Buxton, V. Boyer
Pages 67-88
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- Dominic G. Spinella, James M. Robertson
Pages 110-120
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Nonisotopic Detection
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- Eugene Tu, Edward Jablonski
Pages 142-150
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- Norman C. Nelson, Sherrol H. McDonough
Pages 151-161
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Instrumentation
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- Carl T. Wittwer, Gudrun B. Reed, Kirk M. Ririe
Pages 174-181
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Sequencing
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- Bjorn Andersson, Richard A. Gibbs
Pages 201-213
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- Steve S. Sommer, Erica L. Vielhaber
Pages 214-221
About this book
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Editors and Affiliations
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La Jolla, USA
Kary B. Mullis
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The Immune Response Corporation, Carlsbad, USA
François Ferré
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Institute for Molecular Genetics, Baylor College of Medicine, Houston, USA
Richard A. Gibbs