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Antibody Engineering Protocols

  • Book
  • © 1995

Overview

Editors:
  1. Sudhir Paul

    1. Department of Anesthesiology, University of Nebraska Medical Center, Omaha

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Part of the book series: Methods in Molecular Biology (MIMB, volume 51)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. SEQHUNT

    • George Johnson, Tai Te Wu, Elvin A. Kabat
    Pages 1-15

  3. Molecular Modeling of Antibody-Combining Sites

    • David M. Webster, Anthony R. Rees
    Pages 17-49

  4. Structure and Properties of Human Immunoglobulin Light-Chain Dimers

    • Fred J. Stevens, Marianne Schiffer
    Pages 51-81

  5. Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins

    • Marianne Schiffer, Fred J. Stevens
    Pages 83-98

  6. Detection of Human Variable Gene Family Expression at the Single-Cell Level

    • Moncef Zouali
    Pages 99-110

  7. Purification of Reduced and Alkylated Antibody Subunits

    • Mei Sun, Sudhir Paul
    Pages 111-122

  8. Murine Monoclonal Antibody Development

    • Donald R. Johnson
    Pages 123-137

  9. Comparative Properties of Polyclonal and Monoclonal Antibodies

    • L. Scott Rodkey
    Pages 139-150

  10. Evaluation of Antibody Clonality

    • L. Scott Rodkey
    Pages 151-163

  11. Affinity Immunoblotting

    • Keith A. Knisley, L. Scott Rodkey
    Pages 165-170

  12. Epitope and Idiotope Mapping Using Monoclonal Antibodies

    • Srinivas Kaveri
    Pages 171-181

  13. Anti-Idiotypic Antibodies That Mimic Opioids

    • Jay A. Glasel, Dianne Agarwal
    Pages 183-201

  14. Catalytic Antibodies

    • Howard Amital, Ilan Tur-Kaspa, Zeev Tashma, Israel Hendler, Yehuda Shoenfeld
    Pages 203-210

  15. Preparation and Assay of Acetylcholinesterase Antibody

    • Alain Friboulet, Ladan Izadyar-Demichèle
    Pages 211-222

  16. DNA Hydrolysis by Antibodies

    • Alexander G. Gabibov, Oxana Makarevitch
    Pages 223-235

  17. Screening Strategies for Catalytic Antitransition-State Analog Antibodies

    • Alfonso Tramontano
    Pages 237-250

  18. Expression of Chimeric Immunoglobulin Genes in Mammalian Cells

    • Sergey M. Deyev, Oleg L. Polanovsky
    Pages 251-263

  19. Single-Chain Anti-DNA Fv

    • Michael Polymenis, B. David Stollar
    Pages 265-279

  20. Molecular Cloning of Antiground-State Proteolytic Antibody Fragments

    • Qing-Sheng Gao, Sudhir Paul
    Pages 281-296
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Editors and Affiliations

  • Department of Anesthesiology, University of Nebraska Medical Center, Omaha

    Sudhir Paul

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