Overview
- Editors:
-
-
Barry W. Hicks
-
Department of Chemistry, United States Air Force Academy, Colorado Springs, USA
Access this book
Other ways to access
Table of contents (29 protocols)
-
Green Fluorescent Protein in Transgenic Organisms
-
-
- Ebrahim Shafizadeh, Haigen Huang, Shuo Lin
Pages 225-234
-
-
- C. Neal Stewart Jr., Matthew D. Halfhill, Reginald J. Millwood
Pages 245-252
-
Green Fluorescent Protein Biosensors
-
Front Matter
Pages 253-253
-
- Anikó Váradi, Guy A. Rutter
Pages 255-264
-
- Arjen Schots, Jan M. van der Wolf
Pages 265-273
-
- Scott Ulrich, Kevan Shokat
Pages 275-286
-
- Christopher Coker, Hui Zhao, Harry L. T. Mobley
Pages 287-294
-
Viral Applications of Green Fluorescent Protein
-
Front Matter
Pages 295-295
-
- W. Paul Duprex, Bert K. Rima
Pages 297-307
-
- Jessamyn Bagley, John Iacomini
Pages 309-320
-
- Elisardo Corral Vasquez, Alan Kim Johnson
Pages 321-329
-
- Jonathan E. Loeb, Matthew D. Weitzman, Thomas J. Hope
Pages 331-340
-
- Ravi Mahalingam, Donald H. Gilden
Pages 341-352
-
- Nicoletta Eliopoulos, Jacques Galipeau
Pages 353-371
-
Back Matter
Pages 373-393
About this book
Could there be a better time to be a life scientist? In the past two decades, a host of new techniques have been added to the tool chests of biochemists and molecular biologists. A wonderful benefit of the basic scientific research that fueled the advances in these fields is the wide variety of direct applications in agriculture and medicine. Even with all of these advances, and with the accompanying explosion in computer and information technology, it is clear that the depth of our ignorance vastly exceeds the breadth of our knowledge about complex organisms at the molecular level. Any new techniques or materials that allow us to extend our research-based knowledge should be w- comed and utilized to their fullest potentials. With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented. Although the text has chapters that emphasize different areas of s- cialization, it is not meant to send molecular biologists to one section, botanists to another, and clinicians to still another. Perhaps the most valuable exchange for people in any discipline will come from seeing how others have been able to apply GFP in fields outside of their immediate areas of expertise. GFP from Aequorea victoria is a fluorescent marker protein, and there are certainly other useful fluorophore markers.
Reviews
"The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value." -Doody's Health Sciences Book Review Journal
"...this book truly is a collection of "Applications and protocols" and such is the degree of detail in the methods described that it should perhaps have been sub-titled "A Laboratory Manual." -The Analyst
Editors and Affiliations
-
Department of Chemistry, United States Air Force Academy, Colorado Springs, USA
Barry W. Hicks