Conclusion
We have developed several gene delivery systems. Low molecular weight transfection reagents such as lipoglutamates, lipoglutamides (PEG-lipids) and glycolipids are expected to compact DNA molecule and to control intracellular trafficking of DNA after endocytosis. Chitosan polymer is useful DNA carrier to enhance the expression of gene or antisence DNA. Based on this basic research in vitro, we are going to apply these transfection reagents to gene therapy.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hodgson, C.P. and Solaiman, F., 1996, Nature Biotech., 14: 339–342.
Budker, V., Gurevich, V., Hagstrom, J.E., Bortzov, F. and Wolff, J.A., 1996, Nature Biotech., 14: 760–764.
Liu, Y., Mounkes, L.C., Liggitt, H.D., Brown, C.S., Solodin, I., Heath, T.D. and Debs, R.J., 1997, Nature Biotech., 15, 167–173.
Templeton, N S., Lasic, D.D., Frederik, P.M., Strey, H.H., Roberts, D.D. and Pavlakis, G.N., 1997, Nature Biotech., 15: 647–652.
Sato, T., Akino, H., Okahata, Y., Shoji, Y. and Shimada, J., 1997, J. Biomaterial Science, Polym. Ed., 9: 31–42.
Sato, T., Kawakami, T., Shirakawa, N. and Okahata, Y., 1995, Bull. Chem. Soc. Jpn., 68: 2709–2715.
Sato, T., Kawakami, T., Shirakawa, N., Akino, H. and Okahata, Y., 1996, Biomedical Functions and Biotechnology of Natural and Artificial Polymers, ATL Press, IL., pp. 135–144.
Goldman, C.K., Soroceanu, L., Smith, N., Gillespie, G.Y., Shaw, W., Burgess, S., Bilbao, G. and Curiel, D.T., 1997, Nature Biotech., 15: 462–466.
Sato, T., Akino, H., and Okahata, Y., 1995, Chem. Lett., 755–756.
Sato, T., Akino, H., and Okahata, Y., 1996, Bull. Chem. Soc. Jpn., 69: 2335–2340.
Sato, T. Nishi, H., Okahata, Y., Shoji, Y., and Shimada, J., 1997, Nucleic Acid Symp. Ser., 37: 303–304.
Arvinte, T., and Steponkus, P. L., 1988, Biochemistry, 27: 5671.
Shoji, Y., Akhtar, S., Periasamy, A., Herman, B., and Juliano, R. L., 1991, Nucleic Acids Res., 19: 5543.
R. B. Genis, 1989, Biochemistry, Springer Verlag, NY.
Sato, T. Nishi,, Akino, H., H., Okahata, Y., Shoji, Y., and Shimada, J., 1998, Drug Delivery System, 13: 359–364.
Sato, T., Ishizuka,, T., and Okahata, Y., 1998, Kobunshi Ronbunnshuu, 55: 217–224.
Hirano, S., Seino, H., Akiyama, Y. and Nonaka, I., 1988, Polym. Eng. Sci., 59: 897–901.
Nordtveit, R.J., Vaarum, K..M. and Smidsroed, O., 1996, Carbohydr. Polymers, 29: 163–167.
Aspden, T.J., Illum, L. and Skaugrud, O., 1996, Eur. J. Pharm., 4: 23–31.
Henriksen, I., Sminstad, G. and Karslen, J., 1994, Int. J. Pharm., 101: 227–236.
Imai, T., Shiraishi, S., Saito, H. and Otagiri, M., 1991, Int. J. Pharm., 67: 11–20.
Kristl, J., Smid-Korbac, J., Struc, E., Schara, M. and Rupprecht, H., 1993, Int. J. Pharm., 99: 13–19.
Sato, T., Shirakawa, N., Nishi, H. and Okahata, Y., 1996, Chem. Lett., 725–726.
Venkatesh, S. and Smith, T.J., 1997, Pharm. Dev. Tech., 2: 417–418.
Roy, K., Mao, H-Q, Huang, S-K. and Leong, K.W., 1999, Nature Med., 5: 387–391.
Sato, T., Ishii, T., Okahata, Y., 1999, Proceed. Int’l Symp. Control. Rel. Bioact. Mater., 26: 803–804.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Kluwer Academic Publishers
About this chapter
Cite this chapter
Sato, T., Akino, H., Nishi, H., Ishizuka, T., Ishii, T., Okahata, Y. (2002). In Vitro Gene Delivery by Using Supramolecular Systems. In: Chiellini, E., Sunamoto, J., Migliaresi, C., Ottenbrite, R.M., Cohn, D. (eds) Biomedical Polymers and Polymer Therapeutics. Springer, Boston, MA. https://doi.org/10.1007/0-306-46842-5_29
Download citation
DOI: https://doi.org/10.1007/0-306-46842-5_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46472-0
Online ISBN: 978-0-306-46842-1
eBook Packages: Springer Book Archive