Summary
We established an E. coli fermentation process for plasmid production in 10 L bioreactors yielding between 50–150 mg of unpurified plasmid depending on the plasmid and the host strain.
The E. coli strain was selected to give a high yield of monomeric supercoiled pREP7-TNFRp55 DNA.
A standard alkaline extraction method was scaled up and a 1-step chromatographic procedure yielded plasmid DNA suitable for our transfection protocol.
Transient gene expression with different plasmid preparations exhibited high transfection efficiencies.
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© 1998 Kluwer Academic Publishers
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Schlaeger, E.J., Christensen, K., Schmid, G., Schaub, N., Wipf, B., Weiss, A. (1998). Large Scale Transient Gene Expression in Mammalian Cells. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_19
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DOI: https://doi.org/10.1007/0-306-46860-3_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5016-3
Online ISBN: 978-0-306-46860-5
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