Inhibition of c-jun Expression in F-Mel Cells Causes Cell Cycle Arrest and prevention of Apoptosis

Abstract

We are able to provide an evidence that will support the suppression of cell proliferation to withdrawal from the cell cycle and suppression of onset apoptosis with conditional expression of c-jun antisense transcript in F-MEL cells. F-MEL cells were transfected with c-jun antisense gene located downstream of aglucocorticoid-inducible MMTV promoter, and named as c-jun AS cells. By treating the c-jun AS cells with dexamethasone (DEX)in DMEM supplemented with 10% serum, the growth could be suppressed for duration of 16 days with high cell viability of 92%. When DEX-treated c-jun AS cells were serum deprived, the cell viability remained at high of 86% for upto 10 days, the onset of apoptosis was suppressed, and the internucleosomal cleavage of DNA was not detected upto 8 days. In contrast, when wild type F-MEL cells were serum deprived, the cell viability is low (50%.) and the onset of apoptosis is induced within 2 days, and internucleosomal cleavage of DNA was detectable.