Abstract
Human alpha 2-macroglobulin (hα2M) cDNA was obtained from HepG2 human hepatoma cell line, expression vectors constructed, with which a recombinant ha2M (rhα2M)-producing CHO cell line, rCHO/hα2M, was obtained By N-terminal amino acid sequence determination, purified rhα2M protein obtained was confirmed to be of human type. The profile of conformational change due to chemical and enzymatic reactions, as studied electrophoretically, support that thioester-bonds were properly formed in the rhα2M, as in plasma derived hα2M. In addition, we obtained cDNAs with modified DNA sequence in their ‘bait region’, the region where the specificity for α2M to inhibit a proteinase is determined in accordance with the substrate specificity of the proteinase, and constructed expression vectors, and arecombinant CHO cell line thereof. The potential usefulness of the recombinant wild type-, as well as mutant-, hα2Ms is discussed.
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© 1999 Kluwer Academic Publishers
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Nakamichi, N., Shimizu, M., Ito, M., Ikai, A., Matsumura, T. (1999). Establishment of Recombinant CHO Cell Lines Producing Wild-Type and Mutant Human α2-Macroglobulins. In: Kitagawa, Y., Matsuda, T., Iijima, S. (eds) Animal Cell Technology: Basic & Applied Aspects., vol 1. Springer, Dordrecht. https://doi.org/10.1007/0-306-46865-4_43
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DOI: https://doi.org/10.1007/0-306-46865-4_43
Publisher Name: Springer, Dordrecht
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