Skip to main content
  • 1474 Accesses

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bennett M.D., Cox A.V., Leitch I.J. Angiosperm DNA C-values database http://www.rbgkew.org.uk/cval/databasel.html, 1998

  • Bennett M.D., Leitch I.J. Nuclear DNA amounts in Angiosperms. Ann Bot 1995; 76:113–176

    Article  CAS  Google Scholar 

  • Bennett M.D., Leitch I.J. Nuclear DNA amounts in Angiosperms — 583 new stimates. Ann Bot 1997; 80:169–196

    Article  CAS  Google Scholar 

  • Bennett M.D., Smith J.B. Nuclear DNA amounts in Angiosperms. Philos T Roy Soc B 1976; 274:227–274

    CAS  Google Scholar 

  • Bennett M.D., Smith J.B., Heslop-Harrison J.S. Nuclear DNA amounts in Angiosperms. Proceed Roy Soc London B 1982; 216:179–199

    CAS  Google Scholar 

  • Bennett M.D., Smith J.B. Nuclear DNA amounts in Angiosperms. Philos T Roy Soc B 1991; 334:309–345

    CAS  Google Scholar 

  • Bergounioux C., Brown S.C., Petit P.X. Flow cytometry and plant protoplast cell biology. Physiol Plantarum 1992; 85:374–386

    CAS  Google Scholar 

  • Bergounioux C., Perennes C., Brown S.C., Sarda C., Gadal P. Relation between protoplast division, cell cycle stage and nuclear chromatin structure. Protoplasma 1988a; 142:127–136

    Article  CAS  Google Scholar 

  • Bergounioux C., Perennes C., Brown S.C., Gadal P. Nuclear RNA quantification in protoplast cell-cycle phases. Cytometry 1988b; 9:84–87

    Article  PubMed  CAS  Google Scholar 

  • Bharathan G., Lambert G., Galbraith D.W. Nuclear DNA content of monocotyledons and related taxa. Am J Bot 1994; 81:381–386

    Google Scholar 

  • Blanco A. ContribuciĂłn a la TaxonomĂ­a de Pteridophyta. Minor Thesis. Vigo, Spain: Universidade de Vigo, 1997

    Google Scholar 

  • Brown S.C. Applications of flow cytometry in plant biology and biotechnologies: review and perspectives. Biotech Biotechnol Equipm 1994; 8:75–78

    Google Scholar 

  • Brown S.C., Bergounioux C. “Plant Flow Cytometry.” In Flow Cytometry: Advanced Research and Clinical Applications. Vol II. A. Yen, ed. Boca Raton, Florida: CRC Press, 1989

    Google Scholar 

  • Brown S.C., Bergounioux C., Tallet S., Marie D. “Flow Cytometry of Nuclei for Ploidy and Cell Cycle Analysis.” In A Laboratory Guide for Cellular and Molecular Plant Biology. I. Negrutiu, G. Gharti-Chherti, eds. Birkhäauser: Verlag Basel, 1991a

    Google Scholar 

  • Brown S.C., Devaux P., Marie D., Bergounioux C., Petit P. CytomĂ©trie en flux: application Ă  l’analyse de la ploĂŻdie chez les vĂ©gĂ©taux. Biofutur 1991b

    Google Scholar 

  • Brown S.C., Kouchkovsky F., Marie D. “Polysomaty in Plants.” In The Porto Workshop on Flow and Image Cytometry. A Collection of Ideas. Kimler, O’Connor, Sansonetty, Brown, eds. Porto: IPATIMUP. Series 1, Fundaçao Eng. Antonio Almeida, 1992

    Google Scholar 

  • Brown S.C., Redondo N., Cantrel C., Veuskens A., Horjales M. “Genomesize, a Plant Database.” In Congrès de CytomĂ©trie en Flux et de CytomĂ©trie en Image. France: Association de CytomĂ©trie en Flux Cercle Français de Microscopie Quantitative, 1993

    Google Scholar 

  • Conia J., Bergounioux C., Perennes C., Muller P.H. Flow cytometric analysis and sorting of plant chromosomes from Petunia hybrida protoplasts. Cytometry 1987; 8:500–508

    Article  PubMed  CAS  Google Scholar 

  • Darzynkievicz Z., Juan G. “DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis“ In Currents Protocols in Cytometry. New York, USA: John Wiley and Sons, 1997

    Google Scholar 

  • De Laat A.M.M., Göhde W., Vogelzang M.J.D.C. Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 1987; 99:303–307

    Google Scholar 

  • DoleĹľel J. Flow cytometric analysis of nuclear DNA content in higher plants. Phytochem Anal 1991; 2:143–154

    Google Scholar 

  • DoleĹľel J. Flow cytometry, its Application and Potential for Plant Breeding. In Current Topic in Plant Cytogenetics Related to Plant Improvement. T. Lelley, ed. Vienna: Universitätsverlag, 1998

    Google Scholar 

  • DoleĹľel J., CĂ­halĂ­ková J., Lucretti S. A high-yield procedure for isolation of metaphase chromosomes from root tips of Vicia faba L. Planta 1992; 188:93–98

    Google Scholar 

  • DoleĹľel J., Lucretti S. High-resolution flow karyotyping and chromosome sorting in Vicia faba lines with standard and reconstructed karyotypes. Theor Appl Genet 1995; 90:797–802

    Google Scholar 

  • Galbraith D.W. Analysis of higher plants by flow cytometry and cell sorting. Int Rev Cytol 1989; 116:165–228

    Google Scholar 

  • Galbraith D.W. “Flow Cytometric Analysis of Plant Genomes.” In Methods in Cell Biology. Z. Darzynkiewicz, H. Crissman, eds. London: Academic Press, 1990

    Google Scholar 

  • Galbraith D.W., Harkins K.R., Maddox J.R., Ayres N.M., Sharma D.P., Firoozabady E. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 1983; 220:1049–1051

    CAS  Google Scholar 

  • Galbraith D.W., Lambert G.M., Macas J., DoleĹľel J. “Analysis of Nuclear DNA Content and Ploidy in Higher Plants.” In Currents Protocols in Cytometry. New York, USA: John Wiley and Sons, 1997

    Google Scholar 

  • Godelle B., Cartier D., Marie D., Brown S.C., Siljak-Yakovlev S. Heterochromatin study demonstrating the non-linearity of fluorometry useful for calculating genomic base composition. Cytometry 1993; 14:618–626

    Article  PubMed  CAS  Google Scholar 

  • Horjales M., Redondo N., Blanco A. Datos sobre la cantidad de DNA en el gĂ©nero Equisetum L. NACC 1997; 7: 69–73

    Google Scholar 

  • Jayat C., Ratinaud M.H. Cell cycle analysis by flow cytometry: principles and applications. Biol Cell 1993; 78:15–25

    Article  PubMed  CAS  Google Scholar 

  • Kononowicz A.K., Hasegawa P.M., Bressan R.A. Chromosome number and nuclear DNA content of plants regenerated from salt adapted plant cell. Plant Cell Rep 1990; 8:676–679

    Google Scholar 

  • Lysák M.A., CĂ­halĂ­ková J., Kubaláková M., Simková H., KĂĽnzel G., DoleĹľel J. Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L. Chromosome Res 1999; 7:431–444

    PubMed  Google Scholar 

  • Marie D., Brown S.C. A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biol Cell 1993; 78:41–51

    Article  PubMed  CAS  Google Scholar 

  • MĂ©tĂ©zeau P., Schmitz A., Frelat G. Analysis and sorting of chromosomes by flow cytometry: new trends. Biol Cell 1993; 78:31–39

    PubMed  Google Scholar 

  • Michaelson M.J., Price H.J., Ellison J.R., Johnston J.S. Comparison of plant DNA contents determined by Feulgen microspectrophotometry and laser flow cytometry. Am J Bot 1991; 78:183–188

    CAS  Google Scholar 

  • Petit J.M., Denis-Gay M., Ratinaud M.H. Assessment of fluorochromes for cellular structure and function studies by flow cytometry. Biol Cell 1993; 78:1–13

    Article  PubMed  CAS  Google Scholar 

  • Rabinovitch P.S. “Univariate Cell Cycle Analysis: Principles, Mathematical Modelling and Challenges.” In The Porto Workshop on Flow and Image Cytometry. A Collection of Ideas. Kimler, O’Connor, Sansonetty, Brown, eds. Porto: IPATIMUP. Series 1, Fundaçao Eng. Antonio Almeida, 1992

    Google Scholar 

  • Redondo N., Blanco A., Horjales M. Estudio del gĂ©nero Polypodium L. del Noroeste IbĂ©rico: cantidad de DNA nuclear. NACC 1998; 9:109–116

    Google Scholar 

  • Redondo N., Horjales M., Blanco A. Cantidades de DNA nuclear y esporas Aspleniaceae: Asplenium L., Phyllitis Hill y Ceterach Willd. NACC 1999a; 9:99–107

    Google Scholar 

  • Redondo N., Horjales M., Blanco A. Cantidades de DNA nuclear en helechos del Noroeste IbĂ©rico. Real Sociedad Española de Historia Natural 1999b

    Google Scholar 

  • Steen H.B. “Characteristics of Flow Cytometers.” In Flow Cytometry and Sorting. M.R. Melamed, T. Lindmo, M.L. Mendelsohn, eds. New York, USA: John Wiley and Sons, 1990a

    Google Scholar 

  • Steen H.B. Light scattering measurement in an arc lamp-based flow cytometer. Cytometry 1990b; 11:223–230

    Article  PubMed  CAS  Google Scholar 

  • Tiersch T.R., Chandler R.W., Wachtel S.S., Elias S. Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 1989; 10:706–710

    Article  PubMed  CAS  Google Scholar 

  • Ulrich I., Fritz B., Ulrich W. Application of DNA fluorochromes for flow cytometric DNA analysis of plant protoplasts. Plant Sci 1988; 55:151–158

    Article  CAS  Google Scholar 

  • Veuskens J., Marie D., Hinnisdaels S., Brown S.C. “Flow Cytometry and Sorting of Plant Chromosomes.” In Flow Cytometry and Cell Sorting. A. Radbruch, ed. Heidelberg, Germany: Springer Berlin, 1992

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Manuel J. Reigosa Roger

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Kluwer Academic Publishers

About this chapter

Cite this chapter

Fernández, A.B. (2001). Flow Cytometry: DNA Quantification. In: Reigosa Roger, M.J. (eds) Handbook of Plant Ecophysiology Techniques. Springer, Dordrecht. https://doi.org/10.1007/0-306-48057-3_3

Download citation

  • DOI: https://doi.org/10.1007/0-306-48057-3_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-7053-6

  • Online ISBN: 978-0-306-48057-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics