Abstract
Three Pleurotus strains were grown in a medium containing MSWI fly ash having high concentrations of PCDD/Fs. The supernatants were collected every seven days up to 42 days, and used for the determination of lignin - and manganese-peroxidases (LIP and MnP), and laccase (LAC), and also for toxicity levels. Enzyme activities were determined by spectrophotometric assays. The toxicity levels were determined using a bioassay employing Lettuce (Latuca sativa) seeds. When 2.5 g were used, low levels of LIP, LAC e M nP were detected, regardless of the strain. When Pleurotus sp. CCB 068 was grown in medium containing 3.5 g of fly ash, high LAC activities (+/ - 20 U/L) was detected up to 7th day. Lower activities were observed for P. sajor caju 020 (+/- 15 U/L) e P. tailandia (+/- 10 U/L). P. sp. CCB 068 was the only strain showing a good decrease in the phytotoxic effect after 42 days of treatment (35.90% effect). Our results indicate that P. sp. CCB 068 produced metabolites which were less toxic to the lettuce seeds than the other two strains, and that the higher levels of LAC and MnP produced may play a role in the degradation of PCDD/Fs.
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Dos Reis- Gorny, M., Durrant, L. (2005). Ligninolytic Enzymes and Phytotoxic Effects Following Growth of Strains of Pleurotus in MSWI Fly Ash Contaminated with PCCD/Fs. In: Calabrese, E.J., Kostecki, P.T., Dragun, J. (eds) Contaminated Soils, Sediments and Water. Springer, Boston, MA. https://doi.org/10.1007/0-387-23079-3_22
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