Abstract
The heterotrophic utilization of organic substrates by diatoms is likely an important survival strategy when light levels are too low for photosynthesis. The objectives of this study were: (1) to determine if heterotrophic utilization of a large array of organic compounds by eight common freshwater benthic diatom taxa was light-dependent, and (2) to determine if organic substrate utilization patterns differed between darkgrown diatoms and bacteria as a possible means of reducing competition by niche separation. Eight lightand dark-grown diatom taxa and five bacterial species were incubated in 96-well BiologĀ® Microtiter plates with each well containing 1 of 95 different organic substrates. Oxidation rates of each organic substrate were measured through time. There was a substantial increase in the number of organic substrates oxidized by diatoms grown in the dark compared to their light-grown counterparts, indicating that the transport systems for these molecules may be light activated. Therefore, diatoms likely only utilize these metabolically expensive uptake mechanisms when they are necessary for survival, or when substrates are plentiful. A principal components analysis indicated discernible differences in the types of organic-C substrates utilized by dark-grown diatoms and bacteria. Although bacteria were able to oxidize a more diverse array of organic substrates including carboxylic acids and large polymers, diatoms appeared to more readily utilize the complex carbohydrates. By oxidizing different organic substrates than bacteria, heterotrophically metabolizing diatoms may be reducing direct competition and enhancing coexistence with bacteria.
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References
Allison, R. K., H. E. Skipper, M. R. Reid, W. A. Short & G. L. Hogan, 1953. Studies on the photosynthetic reaction I. The assimilation of acetate by Nostoc muscorum. Journal of Biological Chemistry 204: 197ā205.
Amblard, C., 1991. Carbon heterotrophic activity of microalgae and cyanobacteria ā ecological significance. Annee Biologique 30: 6ā107.
Anitia, N. J., J. Y. Cheng & F. J. R. Taylor, 1969. The heterotrophic growth of a marine photosynthetic cryptomonad (Chroomonas saline). Proceedings of the International Seaweed Symposium 6: 17ā29.
Bertrand, J, 1992. Mouvements des diatomees II-synthese des mouvements. Cryptogamie: Algologie 13: 49ā71.
Burkholder, J. M., R. G. Wetzel & K. L. Klomparens, 1990. Direct comparison of phosphate uptake by adnate and loosely attached microalgae within an intact biofilm matrix. Applied Environmental Microbiology 56: 2882ā2890.
Cole, J. J., 1982. Interactions between bacteria and algae in aquatic ecosystems. Annual Review of Ecology and Systematics 13: 291ā314.
De Lange, H. J., D. P. Morris & C. E. Williamson, 2003. Solar ultraviolet photodegradation of DOC may stimulate freshwater food webs. Journal of Plankton Research 25: 111ā117.
Descy, J. P., B. Leporcq, L. Viroux, C. Francois & P. Servais, 2002. Phytoplankton production, exudation, and bacterial reassimilation in the River Meuse (Belgium). Journal of Plankton Research 24: 161ā166.
Dodds, W. K., 1992. A modified fiber-optic light microprobe to measure spherically integrated photosynthetic photon flux density: Characteristics of periphyton photosynthesisirradiance patterns. Limnology and Oceanography 37: 871ā878.
Flynn, K. J. & T. Butler, 1986. Nitrogen sources for growth of marine microalgae: Role of dissolved free amino acids. Marine Ecology Progress Series 34: 281ā304.
Gurbuz, H. & E. Kivrak, 2003. Seasonal variations of benthic algae of Kuzgun Dam Reservoir and their relationship to environmental factors. Fresenius Environmental Bulleting 12: 1025ā1032.
Hellebust, J. A., 1971. Glucose uptake by Cyclotella cryptica: Dark induction and light inactivation of transport system. Journal of Phycology 7: 345ā349.
Hellebust, J. A. & J. Lewin, 1977. Heterotrophic nutrition. In Werner (ed.) The Biology of Diatoms. University of California Press, Berkeley, 169ā197.
Hudon, C. & E. Bourget, 1981. Initial colonization of artificial substrate: community development and structure studied by scanning electron microscopy. Canadian Journal of Fisheries and Aquatic Sciences 38: 1371ā1384.
Johnson, R. E., N. C. Tuchman & C. G. Peterson, 1997. Changes in the vertical microdistribution of diatoms within a developing periphyton mat. Journal of the North American Benthological Society 16: 503ā519.
Jorgenson, B. B., N. P. Revsbech & Y. Cohen, 1983. Photosynthesis and structure of benthic microbial mats: Microelectrode and SEM studies of four cyanobacterial communities. Limnology and Oceanography 28: 1075ā1093.
Klug, J. L., 2005. Bacterial response to dissolved organic matter affects resource availability for algae. Canadian Journal of Fisheries and Aquatic Sciences 62: 472ā481.
Liehr, S. K., M. T. Suidan & J. W. Eheart, 1990. A modeling study of carbon and light limitation in algal biofilms. Biotechnology and Bioengineering 35: 233ā243.
Liu, M. S. & J. A. Hellebust, 1973. Utilization of amino acids as nitrogen sources, and their effects on nitrate reductase in the marine diatom Cyclotella cryptica. Canadian Journal of Microbiology 20: 1119ā1124.
McCormick, P. V. & R. J. Stevenson, 1991. Mechanisms of benthic algal succession in lotic environments. Ecology 72: 1835ā1848.
Michels, A., 1998. Effects of sewage water on diatoms (Bacillariophyceae) and water quality in two tropical streams in Costa Rica. Revista de BiologĆa Tropical 46: 153ā175.
Neilson, A. H. & R. A. Lewin, 1974. The uptake and utilization of organic carbon by algae; an essay in comparative biochemistry. Phycologia 13: 227ā264.
Nilsson, C. & K. SundbƤck, 1996. Amino acid uptake in natural microphytobenthic assemblages studied by microautoradiography. Hydrobiologia 332: 119ā129.
Paerl, H. W., 1991. Ecophysiological and trophic implications of light-stimulated amino acid utilization in marine picoplankton. Applied Environmental Microbiology 57: 473ā479.
Palmisano, A. C., J. B. SooHoo, D. C. White, G. A. Smith, G. R. Staton & L. H. Burckle, 1985. Shade adapted benthic diatoms beneath Antarctica sea ice. Journal of Phycology 21: 664ā667.
Parker, B. C., H. C. Bold & T. R. Deason, 1961. Facultative heterotrophy in some chlorococcacean algae. Science 133: 761ā763.
Pelczar, M. J., R. Reid & E. C. S. Chan, 1977. Microbiology. McGraw-Hill Book Company, New York, 952 pp.
Petit, M., G. P. Alves & P. Lavandier, 1999. Phytoplankton exudation, bacterial reassimilation and production for three diel cycles in different trophic conditions. Archiv fĆ¼r Hydrobiologie 146: 285ā309.
Puddu, A., A. Zoppini, S. Fazi, M. Rosati, S. Amalfitano & E. Magaletti, 2003. Bacterial uptake of DOM released from Plimited phytoplankton. FEMS Microbial Ecology 46: 257ā268.
Rivkin, R. B. & M. Putt, 1987. Heterotrophy and photoheterotrophy by Antarctic microalgae: Light-dependent incorporation of amino acids and glucose. Journal of Phycology 23: 442ā452.
Schollett, M. A., 1998 Organic nutrient preferences for benthic diatoms: An approach to quantifying heterotrophic metabolism. Masterās Thesis. Loyola University Chicago, Chicago, IL, 91 pp.
Sinsabaugh, R. L. & A. E. Linkins, 1988. Exoenzyme activity associated with lotic epilithon. Freshwater Biology 20: 249ā261.
Stadelmann, E. J., 1962. Permeability. In Lewin, (ed.) Physiology and Biochemistry of Algae. Academic Press, New York, 493ā528.
Steinman, A. D., C. D. McIntire, S. V. Gregory, G. A. Lamberti & L. R. Ashkenas, 1987. Effects of herbivore type and density on taxonomic structure and physiognomy of algal assemblages in laboratory streams. Journal of the North American Benthological Society 6: 175ā188.
Stewart, W. D. P., 1974. Algal Physiology and Biochemistry. University of California Press, Los Angeles, 989 pp.
Tuchman, N. C., 1996. The role of heterotrophy in benthic algae. In Stevenson, R. J. M. Bothwell, & R. Lowe (eds.) Algal Ecology: Freshwater Benthic Habitats. Academic Press, San Diego, 299ā319.
Tuchman, N. C. & R. J. Stevenson, 1991. Effects of selective grazing by snails on benthic algal succession. Journal of the North American Benthological Society 10: 430ā443.
Wasmund, N., 1987. Live algae in deep sediment layers. Internationale Revue der Gesamten Hydrobiologie 74: 589ā597.
Wetzel, R. G., P. G. Hatcher & T. S. Bianchi, 1995. Natural photolysis by ultraviolet irradiance of recalcitrant dissolved organic matter to simple substrates for rapid bacterial metabolism. Limnology and Oceanography 40: 1369ā1380.
Zhang, Q., R. Gradinger & Q. S. Zhou, 2003. Competition within the marine microalgae over the polar dark period in the Greenland Sea of high Arctic. Acta Oceanologica Sinica 22: 233ā242.
Zotina, T., O. Koster & F. Juttner, 2003. Photoheterotrophy and light-dependent uptake of organic and organic nitrogenous compounds by Planktothrix rubescens under low irradiance. Freshwater Biology 48: 1859ā1872.
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Tuchman, N.C., Schollett, M.A., Rier, S.T., Geddes, P. (2006). Differential heterotrophic utilization of organic compounds by diatoms and bacteria under light and dark conditions. In: Stevenson, R.J., Pan, Y., Kociolek, J.P., Kingston, J.C. (eds) Advances in Algal Biology: A Commemoration of the Work of Rex Lowe. Developments in Hydrobiology, vol 185. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5070-4_12
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