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Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems

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Self Organized Nanostructures of Amphiphilic Block Copolymers I

Part of the book series: Advances in Polymer Science ((POLYMER,volume 241))

Abstract

In this article, we outline the principles and application of several time-resolved fluorescence techniques for studying the behavior of stimuli-responsive self-assembled polymer systems. We demonstrate the high research potential of fluorescence using results of several published studies performed by the research team at the Charles University in Prague in the framework of the Marie Curie Research Training Network “Self-Organized Nanostructures of Amphiphilic Copolymers” (MRTN-CT-2003-505027). We have chosen several interesting examples of complex self-assembling systems, the behavior of which could not have been understood without the help of targeted fluorescence studies. We have chosen four different techniques, two of them relatively popular (fluorescence anisotropy and nonradiative excitation energy transfer) and two only little used in polymer science (the solvent relaxation method and fluorescence correlation spectroscopy). The last part of the article is devoted to computer simulations (Monte Carlo and molecular dynamics) aimed at the interpretation of fluorescence data.

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Abbreviations

An:

Anthracene

c.m.c.:

Critical micelle concentration

DLS:

Dynamic light scattering

FCS:

Fluorescence correlation spectroscopy

fwhm:

Full width in half-maximum

IC:

Internal conversion

ISC:

Intersystem crossing, intersystem conversion

LCST:

Lower critical solution temperature

LS:

Light scattering

MC:

Monte Carlo

MD:

Molecular dynamics

M n :

Number-average molar mass

M w :

Weight-average molar mass

NMR:

Nuclear magnetic resonance spectroscopy

Np:

Naphthalene

NRET:

Nonradiative excitation energy transfer

ORB:

Octadecyl rhodamine B

PAA:

Poly(acrylic acid)

PE:

Polyelectrolyte

PEO:

Poly(ethylene oxide)

PMA:

Poly(methacrylic acid)

PS:

Polystyrene

PVP:

Poly(2-vinylpyridine)

rhs:

Right hand side

SLS:

Static light scattering

SRM:

Solvent relaxation method

TCSPC:

Time-correlated single photon counting

TRES:

Time-resolved emission spectra

TRFS:

Time-resolved fluorescence spectroscopy

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Acknowledgements

The authors would like to acknowledge the EU support (Marie Curie MRTN-CT-2003-505027, “POLYAMPHI”), the support of the Ministry of Education, Youths and Sports of the Czech Republic (Research Plan MSM0021620857), the Grant Agency of the Czech Republic (203/07/0659), the Grant Agency of the Academy of Sciences of the Czech Republic (IAA400400621, IAA401110702, and KJB401110701) and the Grant Agency of Charles University (43-257269).

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Authors and Affiliations

  1. Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 12843, Prague 2, Czech Republic

    Karel Procházka, Zuzana Limpouchová, Filip Uhlík, Peter Košovan, Pavel Matějíček, Miroslav Štěpánek, Mariusz Uchman & Jitka Kuldová

  2. The Jaroslav Heyrovsky Institute of Physical Chemistry, Czech Academy of Sciences, Dolejškova 5, Prague 8, Czech Republic

    Radek Šachl, Jana Humpolíčková & Martin Hof

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  1. Karel Procházka
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Correspondence to Karel Procházka .

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Editors and Affiliations

  1. Inst. Makromolekülforschung, LS für Makromolekulare Chemie II, Universität Bayreuth, Universitätsstr. 30, Bayreuth, 95440, Germany

    Axel H. E. Müller

  2. Inst. Pluridisciplinaire de Rech. sur, l'Environnement et les Matériaux (IPREM), UMR CNRS 5254, avenue du President Angot 2, Pau CX 09, 64053, France

    Oleg Borisov

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Procházka, K. et al. (2010). Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems. In: Müller, A., Borisov, O. (eds) Self Organized Nanostructures of Amphiphilic Block Copolymers I. Advances in Polymer Science, vol 241. Springer, Berlin, Heidelberg. https://doi.org/10.1007/12_2010_56

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