Abstract
Substrate electric potential plays an important role in determining the adsorptive behavior of proteins and other macromolecules. In this chapter we describe the measurement of protein adsorption kinetics in the presence of an applied potential using optical waveguide lightmode spectroscopy. We analyze the resulting kinetics in terms of transport- and surface-limited models and show that while substrate potential is an important influencing factor, transport limited by convective diffusion and adsorption in seeming violation of electrostatic principles are prevalent in simple protein systems.
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Van Tassel, P.R. (2006). Protein Adsorption Kinetics: Influence of Substrate Electric Potential. In: Déjardin, P. (eds) Proteins at Solid-Liquid Interfaces. Principles and Practice. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-32658-8_1
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DOI: https://doi.org/10.1007/3-540-32658-8_1
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-32657-1
Online ISBN: 978-3-540-32658-8
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