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Fed-Batch Cultures of Escherichia coli Cells with Oxygen-Dependent nar Promoter Systems

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Tools and Applications of Biochemical Engineering Science

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 74))

Abstract

The recombinant proteins produced from Escherichia coli as a host cell need to be made at as low a cost as possible because of the end of the monopoly following expiry of the patent on early pharmaceutical proteins, and thus expanding applications to non-pharmaceutical large-scale products. We review in this article how the various promoters used in recombinant E. coli could affect its protein products, especially with emphasis on relatively new oxygen-dependent nar promoters for β-galactosidase production. Several studies carried out in the authors’ laboratory show that the nar promoter does not require any chemicals except 1 % nitrate and oxygen for protein production. And according to recent work with the modified strains it is possible to produce the enzyme (β-galactosidase) even without the nitrate ions at 45% of its total protein content when its cell density reached OD = 176.

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References

  1. Georgiou G (1988) AIChE J 34:1233

    Article  CAS  Google Scholar 

  2. Makrides SC (1996) Microbiol Rev 60:512

    CAS  Google Scholar 

  3. Lee SY (1996) Trends Biotechnol 14:98

    Article  CAS  Google Scholar 

  4. Hannig G, Makrides SC (1998) Trends Biotechnol 16:54

    Article  CAS  Google Scholar 

  5. Han SJ, Chang HN, Chang YK, Rhim SL (1996) J Microbiol Biotechnol 6:451

    CAS  Google Scholar 

  6. Strandberg L, Enfors S-O (1991) Biotechnol Lett 13:609

    Article  CAS  Google Scholar 

  7. Hockney RC (1994) Trends Biotechnol 12:456

    Article  CAS  Google Scholar 

  8. Remaut E, Stanssens P, Fiers W (1981) Gene 15:81

    Article  CAS  Google Scholar 

  9. Figge J, Wright C, Collins CJ, Roberts TM, Livingston DM (1988) Cell 52:713

    Article  CAS  Google Scholar 

  10. Carter P, Kelley RF, Rodrigues ML, Snedecor B, Covarrubias M, Velligan MD, Wong WLT, Rowland AM, Kotts CE, Carver ME, Yang M, Bourell JH, Shepard HM, Henner D (1992) Bio/Technology 10:163

    Article  CAS  Google Scholar 

  11. Kasahara M, Makino K, Amemura M, Nakata A, Shinagawa H (1991) J Bacteriol 173:549

    CAS  Google Scholar 

  12. Khosla C, Bailey JE (1988) Nature 331:633

    Article  CAS  Google Scholar 

  13. Chatfield SN, Charles IG, Makoff AJ, Oxer MD, Dougan G, Pickard D, Slater D, Fairweather NF (1992) Bio/Technology 10:888

    Article  CAS  Google Scholar 

  14. Sawers G (1993) Mol Microbiol 10:737

    Article  CAS  Google Scholar 

  15. Wanner BL (1996) Phosphorus assimilation and control of the phosphate regulation. In: Neidhardt FC, Curtiss R III, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella: cellular and molecular biology. ASM Press, Washington, DC, p 1357

    Google Scholar 

  16. Sawers G, Jarsch M (1996) Appl Microbiol Biotechnol 46:1

    Article  CAS  Google Scholar 

  17. Hughes DE, Curtis JE, Khosla C, Bailey JE (1989) Biotechniques 7:1026

    CAS  Google Scholar 

  18. Khosla C, Bailey JE (1989) J Bacteriol 171:5995

    CAS  Google Scholar 

  19. Khosla C, Curtis JE, Bydalek P, Swartz JR, Bailey JE (1990) Bio/Technology 8:554

    Article  CAS  Google Scholar 

  20. Khosla C, Curtis JE, DeModena J, Rinas U, Bailey JE (1990) Bio/Technology 8:849

    Article  CAS  Google Scholar 

  21. Buddenhagen RE, Webster DA, Stark BC (1996) Biotechnol Lett 18:695

    Article  CAS  Google Scholar 

  22. Ko Y-F, Bentley WE, Weigand WA (1993) Biotechnol Bioeng 42:843

    Article  CAS  Google Scholar 

  23. Iuchi S, Lin ECC (1991) Cell 66:5

    Article  CAS  Google Scholar 

  24. Stewart V (1988) Microbiol Rev 52:190

    CAS  Google Scholar 

  25. Gottschalk G (1986) Bacterial metabolism, 2nd edn. Springer, Berlin Heidelberg New York

    Google Scholar 

  26. Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V (1989) Mol Gen Genet 218:249

    Article  CAS  Google Scholar 

  27. Li SF, DeMoss JA (1987) J Bacteriol 169:4614

    CAS  Google Scholar 

  28. Sodergren EJ, DeMoss JA (1988) J Bacteriol 170:1721

    CAS  Google Scholar 

  29. Stewart V (1993) Mol Microbiol 9:425

    Article  CAS  Google Scholar 

  30. Li SF, DeMoss JA (1988) J Biol Chem 263:13,700

    CAS  Google Scholar 

  31. Goodman SD, Nash HA (1989) Nature 343:251

    Article  Google Scholar 

  32. Zhang X, DeMoss JA (1996) J Bacteriol 178:3971

    CAS  Google Scholar 

  33. DeMoss JA (1996) BPERC International Symposium’ 96. Taejon, Korea

    Google Scholar 

  34. Li SF, Rabi T, DeMoss JA (1985) J Bacteriol 164:25

    CAS  Google Scholar 

  35. Lee J, Cho M, Hong E-K, Kim K-S, Lee J (1996) Biotechnol Lett 18:129

    Article  CAS  Google Scholar 

  36. Bonnefoy V, Pascal M-C, Ratouchniak J, Chippaux M (1986) Mol Gen Genet 204:180

    Article  CAS  Google Scholar 

  37. Walker MS, DeMoss JA (1992) J Bacteriol 174:1119

    CAS  Google Scholar 

  38. Lee J (1996) Korean J Biotechnol Bioeng 11:431

    Google Scholar 

  39. Lee J, Cho MH, Lee J (1996) Biotechnol Bioeng 52:572

    Article  CAS  Google Scholar 

  40. Stewart V, MacGregor CH (1982) J Bacteriol 151:788

    CAS  Google Scholar 

  41. Han SJ, Chang HN, Lee J (1998) Biotechnol Bioeng 59:400

    Article  CAS  Google Scholar 

  42. Riesenberg D, Schulz V, Knorre WA, Pohl H-D, Korz D, Sanders EA, Rob A, Deckwer W-D (1991) J Biotechnol 20:17

    Article  CAS  Google Scholar 

  43. Han SJ, Chang HN, DeMoss JA, Suh EJ, Lee J (2000) Biotechnol Bioeng 68:115

    Article  CAS  Google Scholar 

  44. Seeger A, Schneppe B, McCarthy JFG, Deckwer W-D, Rians U (1995) Enzyme Microbiol Tech 17:947

    Article  CAS  Google Scholar 

  45. Schmidt M, Babu KR, Khanna N, Marten S, Rinas U ( 1999), J Biotechnol 68:71

    Article  CAS  Google Scholar 

  46. Babu KR, Swaminathan S, Marten S, Khanna N, Rinas U (2000) Appl Microbiol Biotechnol 53:655

    Article  CAS  Google Scholar 

  47. Labes M, Puhler A, Simon R (1990) Gene 89:37

    Article  CAS  Google Scholar 

  48. Han SJ, Chang HN, Lee JW (2001) Biotechnol Bioeng 72:573

    Article  CAS  Google Scholar 

  49. Han MR (2000) KAIST MS Thesis, Taejon, Korea

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, KAIST, Taejon, 305-701, Korea

    Ho Nam Chang

  2. Samchully Pharmaceutical, Fine Chemical Research Institute, Shiheung, Kyunggi, 429-450, Korea

    Se Jong Han

  3. R&D Center of Coreana Cosmetics, Cheonan, Chungnam, 330-830, Korea

    Mu-ri Han

  4. Department of Biochemistry, School of Medicine, Catholic University of Daegu, Taegu, 705-034, Korea

    Jongwon Lee

Authors
  1. Ho Nam Chang
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  2. Se Jong Han
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  3. Seong-Chun Yim
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  4. Mu-ri Han
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  5. Jongwon Lee
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Editor information

Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstraße 3, 30167, Hannover, Germany

    K. Schügerl

  2. GBF Biochemical Engineering Division, Mascheroder Weg 1, 38124, Braunschweig, Germany

    A.-P. Zeng

Additional information

Dedicated to Prof. Dr. Wolf-Dieter Deckwer on the occasion of his 60th birthday

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© 2002 Springer-Verlag Berlin Heidelberg

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Chang, H.N., Han, S.J., Yim, SC., Han, Mr., Lee, J. (2002). Fed-Batch Cultures of Escherichia coli Cells with Oxygen-Dependent nar Promoter Systems. In: Schügerl, K., et al. Tools and Applications of Biochemical Engineering Science. Advances in Biochemical Engineering/Biotechnology, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45736-4_8

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  • DOI: https://doi.org/10.1007/3-540-45736-4_8

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-42250-1

  • Online ISBN: 978-3-540-45736-7

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