Skip to main content
SpringerLink
Log in

Time-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trends

  • Chapter
  • First Online:
Standardization and Quality Assurance in Fluorescence Measurements II

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 6))

Abstract

Lanthanide, due to their unique luminescent properties, has provided a new tool to make sensitive and robust assay technologies. The long decay time in millisecond time domain allows very easy and efficient temporal gating to get clean and sensitive readings. The various chelate labels and assay technologies developed are reviewed here. Standardization of immunological assays is a major problem in diagnostics, and is addressed in time-resolved immunoassays with ability to create clean signals and standardized readings. The most recent applications of the technologies in preventive screening are given.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Köhler G, Milstein C (1975) Nature 256:495

    Article  Google Scholar 

  2. Soini E, Hemmilä I (1979) Clin Chem 25:353

    CAS  Google Scholar 

  3. Hemmilä I (1991) Applications of Fluorescence in Immunoassays. Wiley Interscience, New York

    Google Scholar 

  4. Weissman SI (1940) J Chem Phys 10:214

    Article  Google Scholar 

  5. Yguerabide J (1972) Methods Enzymol 26:498

    Article  CAS  Google Scholar 

  6. Schneckenburger H (1984) Proc SPIE 491:363

    Google Scholar 

  7. Herman P, Maliwal BP, Lin HJ, Lakowicz JR (2001) J Microsc 203:176

    Article  CAS  Google Scholar 

  8. Hemmilä I, Mukkala V-M (2001) Crit Rev Clin Lab Sci 38:441

    Article  Google Scholar 

  9. Stanley PE (1999) Luminescence 14:201

    Article  CAS  Google Scholar 

  10. Soini E, Kojola H (1983) Clin Chem 29:65

    CAS  Google Scholar 

  11. Diamandis EP (1988) Clin Biohem 21:139

    CAS  Google Scholar 

  12. Seveus L, Väisälä M, Syrjänen S, Sandberg M, Kuusisto A, Harju R, Salo J, Hemmilä I, Kojola H, Soini E (1992) Cytometry 13:329

    Article  CAS  Google Scholar 

  13. Verwoerd NP, Hennink EJ, Bonnet J, van der Geest CR, Tanke HJ (1994) Cytometry 16:113

    Article  CAS  Google Scholar 

  14. Hennink EJ, De Haas R, Verwoerd NP, Tanke HJ (1996) Cytometry 24:312

    Article  CAS  Google Scholar 

  15. Hemmilä I, Dakubu S, Mukkala V-M, Siitari H, Lövgren T (1984) Anal Biochem 137:335

    Article  Google Scholar 

  16. Mukkala V-M, Mikola H, Hemmilä I (1989) Anal Biochem 176:319

    Article  CAS  Google Scholar 

  17. Takalo H, Mukkala V-M, Mikola H, Liitti P, Hemmilä I (1994) Bioconjugate Chem 5:278

    Article  CAS  Google Scholar 

  18. Blomberg K, Granberg C, Hemmilä I, Lövgren T (1986) J Immunol Methods 86:225

    Article  CAS  Google Scholar 

  19. Peuralahti J, Meriö L, Mukkala V-M, Blomberg K, Hovinen J (2006) Bioorg Med Chem Lett 16:4760

    Article  CAS  Google Scholar 

  20. Mathis G (1993) Clin Chem 39:1953

    CAS  Google Scholar 

  21. Li M, Selvin PR (1995) J Am Chem Soc 117:8132

    Article  CAS  Google Scholar 

  22. Mukkala V-M, Helenius M, Hemmilä I, Kankare J, Takalo H (1993) Helv Chim Acta 76:1361

    Article  CAS  Google Scholar 

  23. Cooper ME, Sammes PG (2000) J Chem Soc Perkin Trans 2:1695

    Google Scholar 

  24. Wang Z, Yuan J, Matsumoto K (2005) Luminescence 20:347

    Article  CAS  Google Scholar 

  25. Comley J (2006) Drug Discovery World Spring 2006:22

    Google Scholar 

  26. Härmä H, Soukka T, Lövgren T (2001) Clin Chem 47:561

    Google Scholar 

  27. Huhtinen P, Kivelä M, Kuronen O, Hagren V, Takalo H, Tenhu H, Lövgren T, Härmä H (2005) Anal Chem 77:2643

    Article  CAS  Google Scholar 

  28. Valanne A, Huopalahti S, Soukka T, Vaininpää R, Lövgren T, Härmä H (2005) J Clin Virol 33:217

    CAS  Google Scholar 

  29. Cummins CM, Koivunen ME, Stephanian A, Gee SJ, Hammock BD, Kennedy IM (2006) Biosens Bioelectron 21:1077

    Article  CAS  Google Scholar 

  30. Hemmilä I, Laitala V (2005) J Fluorescence 15:529

    Article  CAS  Google Scholar 

  31. Laitala V, Hemmilä I (2005) Anal Chem 77:1483

    Article  CAS  Google Scholar 

  32. Laitala V, Hemmilä I (2005) Anal Chim Acta 551:73

    Article  CAS  Google Scholar 

  33. Mathis G (1993) Clin Chem 39:1953

    CAS  Google Scholar 

  34. Kimura H, Matsumoto K, Mukaida M (2005) J Anal Toxicol 29:799

    CAS  Google Scholar 

  35. Qin Q-P, Peltola O, Pettersson K (2003) Clin Chem 49:1105

    Article  CAS  Google Scholar 

  36. Zhang WX, Wang R, Wisniewski D, Marcy AI, LoGrasso P, Lisnock J-M, Cummings RT, Thompson JE (2005) Anal Biochem 343:76

    Article  CAS  Google Scholar 

  37. Leifert WR, Bailey K, Cooper TH, Aloia AL, Glatz RV, McMurchie EJ (2006) Anal Biochem 355:201

    Article  CAS  Google Scholar 

  38. Panteghini M, Forest JC (2005) Clin Chim Acta 355:1

    Article  CAS  Google Scholar 

  39. Stenman U-H (2001) Clin Chem 47:815

    CAS  Google Scholar 

  40. Barker PE, Wagner PD, Stein SE, Bunk DM, Srivastava S, Omenn GS (2006) Clin Chem 52:1669

    Article  CAS  Google Scholar 

  41. Chalet EA, Travaglino P, Pagani S, Bozzola E, Marino E, Berensztein E, Maceiras M, Tauber M, Rivarola MA, Belgorosky A, Bozzola M (2006) J Endocrinol Invest 29:109

    Google Scholar 

  42. Taimela E, Kairisto V, Koskinen P, Leino A, Irjala K (1997) Eur J Clin Chem Clin Biochem 35:889

    CAS  Google Scholar 

  43. Giton F, Fiet J, Guéchot J, Ibrahim F, Bronsard F, Chopin D, Raynaud J-P (2006) Clin Chem 52:474

    Article  CAS  Google Scholar 

  44. Selva DM, Bassas L, Munell F, Mata A, Tekpetey F, Lewis JG, Hammond GL (2005) J Clin Endocrinol Metab 90:6275

    Article  CAS  Google Scholar 

  45. Ulmert D, Becker C, Nilsson J-Å, Piironen T, Björk T, Hugosson J, Berglund G, Lilja H (2006) Clin Chem 52:235

    Article  CAS  Google Scholar 

  46. Wittfooth S, Qin Q-P, Lund J, Tierala I, Pulkki K, Taralo H, Pettersson K (2006) Clin Chem 52:1794

    Article  CAS  Google Scholar 

  47. Markkanen H, Pekkarinen T, Välimäki MJ, Alfthan H, Kauppinen-Mäkelin R, Sane T, Stenman U-H (2006) Clin Chem 52:468

    Article  CAS  Google Scholar 

  48. Van der Kamp HJ, Oudshoorn CGM, Elvers BH, Van Baarle M, Otten BJ, Wit JM, Verkerk PH (2005) J Clin Endocrinol Metab 90:3904

    Article  CAS  Google Scholar 

  49. Gibney J, Smith TP, McKenna TJ (2005) J Clin Endocrinol Metab 90:3927

    Article  CAS  Google Scholar 

  50. Eriksson S, Halenius H, Pulkki K, Hellman J, Pettersson K (2005) Clin Chem 51:839

    Article  CAS  Google Scholar 

  51. Eriksson S, Ilva T, Becker C, Lund J, Porela P, Pulkki K, Voipio-Pulkki L-M, Pettersson K (2005) Clin Chem 51:848

    Article  CAS  Google Scholar 

  52. Warren DJ, Bjerner J, Paus E, Børmer OP, Nustad K (2005) Clin Chem 51:830

    Article  CAS  Google Scholar 

  53. Fuglsang J, Sandager P, Møller N, Fisker S, Ørskov H, Ovesen P (2006) Eur J Endocrinol 154:449

    Article  CAS  Google Scholar 

  54. Ish-Shalom E, Gargir A, André S, Borovsky Z, Ochanuna Z, Gabius H-J, Tykocinski ML, Ramichmilewitz J (2006) Glycobiology 16:173

    Article  CAS  Google Scholar 

  55. Cabar FR, Pereira PP, Schultz R, Zugaib M (2006) Hum Reprod 21:2426

    Article  CAS  Google Scholar 

  56. Raile K, Klammt J, Schneider A, Keller A, Laue S, Smith R, Pfäffle R, Kratzsch J, Keller E, Kiess W (2006) J Clin Endocrinol Metab 91:2264

    Article  CAS  Google Scholar 

  57. Walenkamp MJ, van der Kamp HJ, Pereira AM, Kant SG, Van Duyvenvoorde HA, Kruithof MF, Breuning MH, Romijn JA, Karperien M, Wit JM (2006) J Clin Endocrinol Metab 91:3062

    Article  CAS  Google Scholar 

  58. Laigaard J, Sørensen T, Placing S, Holck P, Fröhlich C, Wøjdemann KR, Sundberg K, Shalmi A-C, Tabor A, Nørgaard-Pedersen B, Ottesen B, Christiansen M, Wewer UM (2005) Obstet Gynecol 106:144

    Article  CAS  Google Scholar 

  59. Qin Q-P, Kokkala S, Lund J, Tamm N, Voipio-Pulkki L-M, Pettersson K (2005) Clin Chem 51:75

    Article  CAS  Google Scholar 

  60. Wojdemann KR, Larsen SO, Rode L, Shami A, Sundberg K, Christiansen M, Tabor A (2006) Scand J Clin Lab Invest 66:101

    Article  CAS  Google Scholar 

  61. Weinans MJN, Sancken U, Pandian R, Van de Ouweland JMW, De Bruijn HWA, Holm JP, Mantingh A (2005) Clin Chem 51:1276

    Article  CAS  Google Scholar 

  62. Buimer M, Van Wassenaer AG, Ganzevoort W, Wolf H, Bleker OP, Kok JH (2005) Obstet Gynecol 106:973

    Article  Google Scholar 

  63. Brix TH, Hansen PS, Rudbeck AB, Hansen JB, Skytthe A, Kyvik KO, Hegedüs L (2006) J Clin Endocrinol Metab 91:3499

    Article  CAS  Google Scholar 

  64. Kempers MJE, Van Trotsenburg ASP, Van Tijn DA, Bakker E, Wiedijk BM, Endert E, De Vijlder JM, Vulsma T (2005) J Clin Endocrinol Metab 90:4094

    Article  CAS  Google Scholar 

  65. Wekking EM, Appelhof BC, Fliers E, Schene AH, Huyser J, Tijssen JGP, Wiersinga WM (2005) Eur J Endocrinol 153:747

    Article  CAS  Google Scholar 

  66. McElduff A, McElduff P, Wiley V, Wilcken B (2005) J Clin Endocrinol Metab 90:6361

    Article  CAS  Google Scholar 

  67. Schmidt DR, Hogh B, Andersen O, Fuchs J, Fledelius J, Petersen E (2006) Arch Dis Child 91:661

    Article  CAS  Google Scholar 

  68. Taimela E, Aalto M, Viikari P, Nuutila J, Irjala K (1995) Scand J Clin Lab Invest 55:537

    Article  CAS  Google Scholar 

  69. Hebles-Duvison M, Cruz-Ruiz M, Vazquez-Rubio R, Macarro-Sancho I, Silva-Mejias C, Recio-Quijano F (1996) Clin Chem 42:670

    Google Scholar 

  70. Tylek-Lománska D, Kumorowicz-Kopiek M, Starzyk J (2006) J Med Screening 12:166

    Article  Google Scholar 

  71. Votava F, Török D, Kováks J, Möslinger D, Baumgartner-Parzer SM, Sólyom J, Pribilincová Z, Battelino T, Lebl J, Frisch H, Waldhauser F (2005) Eur J Endocrinol 152:869

    Article  CAS  Google Scholar 

  72. Johannsen TH, Mallet D, Dige-Petersen H, Müller J, Main KM, Morel Y, Forest MG (2005) J Clin Endocrinol Metab 90:2056

    Article  CAS  Google Scholar 

  73. Parkinson-Lawrence E, Fuller M, Hopwood JJ, Meikle PJ, Brooks DA (2006) Clin Chem 52:1660

    Article  CAS  Google Scholar 

  74. Fuller M, Lovejoy M, Brooks DA, Harkin ML, Hopwood JJ, Meikle PJ (2004) Clin Chem 50:1979

    Article  CAS  Google Scholar 

  75. Fuller M, Lovejoy M, Hopwood JJ, Meikle PJ (2005) Clin Chem 51:2200

    Article  CAS  Google Scholar 

  76. Fuller M, Brooks DA, Evangelista M, Hein LK, Hopwood JJ, Meikle PJ (2005) Mol Gen Metab 84:18

    Article  CAS  Google Scholar 

  77. Hein LK, Meikle PJ, Dean CJ, Bockmann MR, Auclair D, Hopwood JJ, Brooks DA (2005) Clin Chim Acta 353:67

    Article  CAS  Google Scholar 

  78. Dean CJ, Bockmann MR, Hopwood JJ, Brooks DA, Meikle PJ (2006) Clin Chem 52:643

    Article  CAS  Google Scholar 

  79. Parkinson-Lawrence EJ, Dean CJ, Chang M, Hopwood JJ, Meikle PJ, Brooks DA (2005) Cell Immunol 236:161

    Article  CAS  Google Scholar 

  80. Piironen T, Häse A, Huland H, Steuber T, Christensen IJ, Brünner N, Danø K, Høyer-Hansen G, Lilja H (2006) Clin Chem 52:838

    Article  CAS  Google Scholar 

  81. Aus G, Damber J-E, Khatami A, Lilja H, Stranne J, Hugosson J (2005) Arch Int Med 165:1857

    Article  Google Scholar 

  82. Safarinejad MR (2006) Ann Onc 17:1166

    Article  CAS  Google Scholar 

  83. Resnati M, Pallavicini I, Daverio R, Sidenius N, Bonini P, Blasi F (2006) J Immunol Methods 308:192

    Article  CAS  Google Scholar 

  84. Fernandes LC, Kim SB, Saad SS, Matos D (2006) World J Gastroenerol 12:3891

    CAS  Google Scholar 

  85. Laaksonen DE, Niskanen L, Punnonen K, Nyyssönen K, Tuomainen T-P, Valkonen V-P, Salonen JT (2005) J Clin Endocrinol Metab 90:712

    Article  CAS  Google Scholar 

  86. Kalme T, Seppälä M, Qing Q, Koistinen R, Nissinen A, Harrela M, Loukovaara M, Leinonen P, Tuomilehto J (2005) J Clin Endocrinol Metab 90:1550

    Article  CAS  Google Scholar 

  87. Valverde-Franco G, Binette GS, Li W, Wang H, Chai S, Laflamme F, Tran-Khanh N, Quenneville E, Meijers T, Poole AR, Mort JS, Bushmann MD, Henderson JE (2006) Hum Mol Genet 15:1783

    Article  CAS  Google Scholar 

  88. Ivaska KK, Käkönen S-M, Gerdhem P, Obrant KJ, Pettersson K, Väänänen HK (2005) Clin Chem 51:618

    Article  CAS  Google Scholar 

  89. Sipilä S, Heikkinen E, Cheng S, Suominen H, Saari P, Kovanen V, Alén M, Rantanen T (2006) J Gerontol A Biol Sci Med Sci 61:92

    Google Scholar 

  90. Heijckmann AC, Huiberts MSP, Geusens P, De Vries J, Menheere PPCA, Wolffenbuttel BHR (2005) Eur J Endocrinol 153:23

    Article  CAS  Google Scholar 

  91. Lu PH, Masterman DA, Mulnard R, Cotman C, Miller B, Yaffe K, Reback E, Porter V, Swerdloff R, Cummings JL (2005) Arch Neurol 63:177

    Article  Google Scholar 

  92. Cancellotti E, Wiseman F, Tuzi NL, Baybutt H, Monaghan P, Aitchison L, Simpson J, Manson JC (2005) J Biol Chem 280:42909

    Article  CAS  Google Scholar 

  93. Hemmilä I, Laitala V (2005) J Fluorescence 15:529

    Article  CAS  Google Scholar 

  94. Glaser SM, Hughes IE, Hopp JR, Hathaway K, Perret D, Reff ME (2005) J Biol Chem 280:41494

    Article  CAS  Google Scholar 

  95. Pulli T, Höyhtyä M, Söderlund H, Takkinen K (2005) Anal Chem 77:2637

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. PerkinElmer Life and Analytical Sciences, Wallac Oy, PO Box 10, FIN 20101, Turku, Finland

    Ilkka Hemmilä

Authors
  1. Ilkka Hemmilä
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ilkka Hemmilä .

Editor information

Ute Resch-Genger

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Hemmilä, I. (2008). Time-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trends. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_038

Download citation

Publish with us

Policies and ethics

Search

Navigation