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The Contrast Formation in Optical Microscopy

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Handbook Of Biological Confocal Microscopy

Abstract

In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to “see” the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image.

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Authors and Affiliations

  1. State University of New York at Buffalo, Buffalo, New York, 14260

    Ping-Chin Cheng

  2. National University of Singapore, Singapore

    Ping-Chin Cheng

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  1. Ping-Chin Cheng
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Editors and Affiliations

  1. Department of Zoology, University of Wisconsin, Madison, WI, 53706, USA

    James B. Pawley

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Cheng, PC. (2006). The Contrast Formation in Optical Microscopy. In: Pawley, J. (eds) Handbook Of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-45524-2_8

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