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Label-Free Fingerprinting of Pathogens by Raman Spectroscopy Techniques

  • Chapter
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems

Abstract

Raman spectroscopy is a label-free technique for generating unique spectral fingerprints from intact microorganisms. Studies conducted for more than a decade have shown that these “whole-organism fingerprints” can be used to identify pathogens, including bacteria, yeasts, and spores, at the strain level, even when the microorganisms are so closely related that they are difficult to distinguish by conventional techniques. Emerging techniques such as Raman microscopy and surface-enhanced Raman scattering (SERS) can enhance the magnitude of the signal to the point that Raman fingerprinting can achieve single-cell sensitivity. More recently, Raman microscopy and SERS have been integrated with biomolecule capture to produce a new microarray technology, dubbed “microSERS,” for rapid identification of pathogens and their toxins in complex samples, without any labels, pre-processing of the sample, or culturing. This chapter reviews the studies that have been done on Raman microscopy and SERS for pathogen identification, and innovative methods for sample collection, concentration, and manipulation that can be combined with fingerprinting techniques. It also presents recent progress on microSERS analysis for the identification of bacteria, spores, and toxins in complex samples; differentiation between viable and nonviable microorganisms; and evaluation of growth conditions on microbial phenotype and specificity/affinity for capture biomolecules.

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References

  • Alekseev AN, Karabanova LN, Krainova OA, Krasov ES and Kashparova EV (1985) Amino acid and mineral element content and the activity of various enzymes in germinating spores of Bacillus thuringiensis. Mikrobiologiia 54(2):181–185

    Google Scholar 

  • Alexander TA, Pellegrino PM and Gillespie JB (2003) Near-infrared surface-enhanced-Raman-scattering-mediated detection of single optically trapped bacterial spores. Appl. Spectrosc. 57(11):1340–1345

    Article  Google Scholar 

  • Annous BA, Becker LA, Bayles DO, Labeda DP and Wilkinson BJ (1997) Critical role of anteiso-C15:0 fatty acid in the growth of Listeria monocytogenes at low temperatures. Appl. Environ. Microbiol. 63(10):3887–3894

    Google Scholar 

  • Basher HA, Fowler DR, Rodgers FG, Seaman A and Woodbine M (1984) Role of haemolysin and temperature in the pathogenesis of Listeria monocytogenes in fertile hens’ eggs. Zentralbl. Bakteriol. Mikrobiol. Hyg. [A] 258(2–3):223–231

    Google Scholar 

  • Beaman TC and Gerhardt P (1986) Heat resistance of bacterial spores correlated with protoplast dehydration, mineralization, and thermal adaptation. Appl. Environ. Microbiol. 52:1242–1246

    Google Scholar 

  • Beaman TC, Koshikawa T, Pankratz HS and Gerhardt P (1984) Dehydration partitioned within core protoplast accounts for heat resistance of bacterial spores. FEMS Microbiol. Lett. 24:47–51

    Article  Google Scholar 

  • Bekhtereva MN, Marchenko IV, Galanina LA and Loginova ON (1975) Change in Bacillus anthracoides spores and their content of dipicolinic acid during germination. Mikrobiologiia 44(2):233–236

    Google Scholar 

  • Berger AJ and Zhu Q (2003) Identification of oral bacteria by Raman microspectroscopy. J. Modern Opt. 50(15-17):2375–2380

    Article  Google Scholar 

  • Boschwitz H, Gofshtein-Gandman L, Halvorson HO, Keynan A and Milner Y (1991) The possible involvement of trypsin-like enzymes in germination of spores of Bacillus cereus T and Bacillus subtilis 168. J. Gen. Microbiol. 137(Pt 5):1145–1153

    Google Scholar 

  • Buttingsrud B and Alsberg BK (2004) A new maximum entropy-based method for deconvolution of spectra with heteroscedastic noise. J. Chemometrics 18(12):537–547

    Article  Google Scholar 

  • Byrne B and Swanson MS (1998) Expression of Legionella pneumophila virulence traits in response to growth conditions. Infect. Immun. 66(7):3029–3034

    Google Scholar 

  • Callender R and Deng H (1994) Nonresonance Raman difference spectroscopy: a general probe of protein structure, ligand binding, enzymatic catalysis, and the structures of other biomacromolecules. Annu. Rev. Biophys. Biomol. Struct. 23:215–245

    Article  Google Scholar 

  • Callender R, Deng H and Gilmanshin R (1998) Raman difference studies of protein structure and folding, enzymatic catalysis and ligand binding. J. Raman Spectrosc. 29:15–21

    Article  Google Scholar 

  • Carey PR (2006) Raman crystallography and other biochemical applications of Raman microscopy. Annu. Rev. Phys. Chem. 57: 527–554

    Article  Google Scholar 

  • Cazemier AE, Wagenaars SFM and ter Steeg PF (2001) Effect of sporulation and recovery medium on the heat resistance and amount of injury of spores from spoilage bacilli. J. Appl. Microbiol. 90:761–770

    Article  Google Scholar 

  • Chan JW, Esposito AP, Talley CE, Hollars CW, Lane SM and Huser T (2004) Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy. Anal. Chem. 76(3):599–603

    Article  Google Scholar 

  • Choo-Smith LP, Maquelin K, van Vreeswijk T, Bruining HA, Puppels GJ, Ngo Thi NA, Kirschner C, Naumann D, Ami D, Villa AM, Orsini F, Doglia SM, Lamfarraj H, Sockalingum GD, Manfait M, Allouch P and Endtz HP (2001) Investigating microbial (micro)colony heterogeneity by vibrational spectroscopy. Appl. Environ. Microbiol. 67(4):1461–1469

    Article  Google Scholar 

  • Cowart RE, Lashmet J, McIntosh ME and Adams TJ (1990) Adherence of a virulent strain of Listeria monocytogenes to the surface of a hepatocarcinoma cell line via lectin-substrate interaction. Arch. Microbiol. 153(3):282–286

    Article  Google Scholar 

  • Czuprynski CJ, Brown JF and Roll JT (1989) Growth at reduced temperatures increases the virulence of Listeria monocytogenes for intravenously but not intragastrically inoculated mice. Microb. Pathog. 7(3):213–23

    Article  Google Scholar 

  • De Gussem K, Vandenabeele P, Verbeken A and Moens L (2005) Raman spectroscopic study of Lactarius spores (Russulales, Fungi). Spectrochim. Acta A 61:2896–2908

    Article  Google Scholar 

  • Driskell JD, Kwarta KM, Lipert RJ and Porter MD (2005) Low-level detection of viral pathogens by a surface-enhanced Raman scattering based immunoassay. Anal. Chem. 77:6147–6154

    Article  Google Scholar 

  • Durst J (1975) The role of temperature factors in the epidemiology of listeriosis. Zentralbl. Bakteriol. [Orig. A] 233(1):72–74

    Google Scholar 

  • Efrima S, Bronk BV and Czégé J (1999) Surface enhanced Raman spectroscopy of bacteria coated by silver. Proc. SPIE 3602:164–171

    Article  Google Scholar 

  • Eichenbaum Z, Green BD and Scott JR (1996) Iron starvation causes release from the group A streptococcus of the ADP-ribosylating protein called plasmin receptor or surface glyceraldehyde-3-phosphate-dehydrogenase. Infect. Immun. 64(6):1956–1960

    Google Scholar 

  • Escoriza MF, Van Briesen JM, Stewart S, Maier J and Treado PJ (2006) Raman spectroscopy and chemical imaging for quantification of filtered waterborne bacteria. J. Microbiol. Methods 66(1):63–72

    Article  Google Scholar 

  • Esposito AP, Talley CE, Huser T, Hollars CW, Schaldach CM and Lane SM (2003) Analysis of single bacterial spores by micro-Raman spectroscopy. Appl. Spectrosc. 57:868–871

    Article  Google Scholar 

  • Facinelli B, Giovanetti E, Magi G, Biavasco F and Varaldo PE (1998) Lectin reactivity and virulence among strains of Listeria monocytogenes determined in vitro using the enterocyte-like cell line Caco-2. Microbiol. 144(Pt 1):109–118

    Google Scholar 

  • Gilot P, Andre P and Content J (1999) Listeria monocytogenes possesses adhesins for fibronectin. Infect. Immun. 67(12):6698–6701

    Google Scholar 

  • Gilot P, Jossin Y and Content J (2000) Cloning, sequencing and characterisation of a Listeria monocytogenes gene encoding a fibronectin-binding protein. J. Med. Microbiol. 49(10):887–896

    Google Scholar 

  • Goldfarb WB and Margraf H (1967) Cyanide production by Pseudomonas aeruginosa. Ann. Surg. 165(1):104–110

    Article  Google Scholar 

  • González I, López M, Matnez S, Bernardo A and González J (1999) Thermal inactivation of Bacillus cereus spores formed at different temperatures. Int. J. Food Microbiol. 51:81–84

    Article  Google Scholar 

  • Goodacre R, Timmins EM, Burton R, Kaderbhai N, Woodward AM, Kell DB and Rooney PJ (1998) Rapid identification of urinary tract infection bacteria using hyperspectral whole-organism fingerprinting and artificial neural networks. Microbiology 144:1157–1170

    Google Scholar 

  • Gray PC, Shokair I, Rosenthal S, Tisone GC, Wagner JS, Rigdon LD, Siragusa GR and Heinin RJ (1998) Distinguishability of biological material using ultraviolet multi-spectral fluorescence. Appl. Opt. 37:6037–6041

    Article  Google Scholar 

  • Grow AE (1999) Raman optrode processes and devices for detection of chemicals and microorganisms. U.S. Patent No. 5,866,430

    Google Scholar 

  • Grow AE (2001) SBIR Phase I Final Report, Contract No. 50-DKNA-0-90046. U.S. Department of Commerce, NOAA

    Google Scholar 

  • Grow AE (2002) SBIR Phase I Final Report, Grant No. 1R43ES11226-01. NIEHS

    Google Scholar 

  • Grow AE, Wood L, Deal M, Claycomb J, Lee S and Thompson P (2002) SBIR Phase II Final Report, Contract No. NAS5-00222. NASA

    Google Scholar 

  • Grow AE, Wood LL, Claycomb JL and Thompson PA (2003a) New biochip technology for label-free detection of pathogens and their toxins. J. Microbiol. Methods. 53(2):221–233

    Article  Google Scholar 

  • Grow AE, Deal MS, Thompson PA and Wood LL (2003b) Evaluation of the Doodlebug: A Biochip for Detecting Waterborne Pathogens. IWA Publishing, London

    Google Scholar 

  • Guicheteau JA and Christesen SD (2004) Surface-enhanced Raman immunoassay (SERIA): detection of Bacillus globigii in ground water. Proc. SPIE 5585:113–121

    Article  Google Scholar 

  • Guicheteau J and Christesen SD (2007) Principal component analysis of bacteria using surface-enhanced Raman spectroscopy. Proc. SPIE 6218:62180G

    Article  Google Scholar 

  • Helgason E, Okstad OA, Caugant DA, Johansen HA, Fouet A, Mock M, Hegna I and Kolsto A-B (2000) Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis–one species on the basis of genetic evidence. Appl. Environ. Microbiol. 66:2627–2630

    Article  Google Scholar 

  • Helm D, Labischinski H, Schallehn G and Naumann D (1991) Classification and identification of bacteria by Fourier transform infrared spectroscopy. J. Gen. Microbiol. 137:69–79

    Google Scholar 

  • Holt C, Hirst D, Sutherland A and MacDonald F (1995) Discrimination of species in the genus Listeria by Fourier transform infrared spectroscopy and canonical variate analysis. Appl. Environ. Microbiol. 61(1):377–378

    Google Scholar 

  • Hosseini H, Ghaffariyeh A and Nikandish R (2007) Noxious compounds in exhaled air, a potential cause for ocular manifestations of H. pylori gastrointestinal infection. Med. Hypotheses 68(1):91–93

    Article  Google Scholar 

  • Hou D, Maheshwari S and Chang H-C (2007) Rapid bio-particle concentration and detection by combining a discharge driven vortex with surface-enhanced Raman scattering. Biomicrofluidics 1:014106–014118

    Article  Google Scholar 

  • Huang WE, Griffiths RI, Thompson IP, Bailey MJ and Whiteley AS (2004) Raman microscopic analysis of single microbial cells. Anal. Chem. 76:4452–4458

    Article  Google Scholar 

  • Huang YS, Karashima T, Yamamoto M and Hamaguchi HO (2005) Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman spectroscopy. Biochemistry 44(30):10009–10019

    Article  Google Scholar 

  • Huang WE, Bailey MJ, Thompson IP, Whiteley AS and Spiers AJ (2007) Single-cell Raman spectral profiles of Pseudomonas fluorescens SBW25 reflects in vitro and in planta metabolic history. Microbial Ecology 53:414–425

    Article  Google Scholar 

  • Hutsebaut D, Maquelin K, De Vos P, Vandenabeele P, Moens L and Puppels GJ (2004) Effect of culture conditions on the achievable taxonomic resolution of Raman spectroscopy disclosed by three Bacillus species. Anal. Chem. 76(21):6274–6281

    Article  Google Scholar 

  • Hutsebaut D, Vandroemme J, Heyrman J, Dawyndt P, Vandenabeele P, Moens L and de Vos P (2006) Raman microspectroscopy as an identification tool within the phylogenetically homogeneous “Bacillus subtilis”-group. Syst. Appl. Microbiol. 29(8):650–660

    Article  Google Scholar 

  • Ibelings MS, Maquelin K, Endtz HP, Bruining HA and Puppels GJ (2005) Rapid identification of Candida spp. in peritonitis patients by Raman spectroscopy. Clin. Microbiol. Infect. 11(5):353–358

    Article  Google Scholar 

  • James BW, Mauchline WS, Fitzgeorge RB, Dennis PJ and Keevil CW (1995) Influence of iron-limited continuous culture on physiology and virulence of Legionella pneumophila. Infect. Immun. 63(11):4224–4230

    Google Scholar 

  • James BW, Mauchline WS, Dennis PJ and Keevil CW (1997) A study of iron acquisition mechanisms of Legionella pneumophila grown in chemostat culture. Curr. Microbiol. 34(4):238–243

    Article  Google Scholar 

  • Jarvis RM and Goodacre R (2004) Discrimination of bacteria using surface-enhanced Raman spectroscopy. Anal. Chem. 76(1):40–47

    Article  Google Scholar 

  • Jarvis RM, Brooker A and Goodacre R (2004) Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface. Anal. Chem. 76(17):5198–5202

    Article  Google Scholar 

  • Jarvis RM and Goodacre R (2005) Genetic algorithm optimization for pre-processing and variable selection of spectroscopic data. Bioinformatics 21(7):860–868

    Article  Google Scholar 

  • Jarvis RM, Brooker A and Goodacre R (2006) Surface-enhanced Raman scattering for the rapid discrimination of bacteria. Faraday Discus. 132:281–292

    Article  Google Scholar 

  • Kapatral V, Olson JW, Pepe JC, Miller VL and Minnich SA (1996) Temperature-dependent regulation of Yersinia enterocolitica Class III flagellar genes. Mol. Microbiol. 19(5):1061–1071

    Article  Google Scholar 

  • Kirschner C, Maquelin K, Pina P, Ngo Thi NA, Choo-Smith L-P, Sockalingum GD, Sandt C, Ami D, Orsini F, Doglia SM, Allouch P, Mainfait M, Puppels GJ and Naumann D (2001) Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. J. Clin. Microbiol. 39(5):1763-1770

    Article  Google Scholar 

  • Kneipp K, Wang Y, Kneipp H, Perelman LT, Itzkan I, Dasari RR and Feld MS (1997) Single molecule detection using surface-enhanced Raman scattering. Phys. Rev. Lett. 78:1667–1670

    Article  Google Scholar 

  • Kneipp K, Kneipp H, Kartha VB, Manoharan R, Deinum G, Itzkan I, Dasari RR and Feld MS (1998a) Detection and identification of a single DNA base molecule using surface-enhanced Raman scattering (SERS). Phys. Rev. E 57, Rapid Comm., R6281

    Google Scholar 

  • Kneipp K, Kneipp H, Manoharan R, Hanlon EB, Itzkan I, Dasari RR and Feld MS (1998b) Extremely large enhancement factors in surface-enhanced Raman scattering for molecules on colloidal gold clusters. Appl. Spectrosc. 52:1493–1497

    Article  Google Scholar 

  • Kneipp K, Kneipp H, Itzkan I, Dasari RR and Feld MS (1999) Surface-enhanced non-linear Raman scattering at the single molecule level. Chem. Phys. 247:155–162

    Article  Google Scholar 

  • Kozuka S, Yasuda Y and Tochikubo K (1985) Ultrastructural localization of dipicolinic acid in dormant spores of Bacillus subtilis by immunoelectron microscopy with colloidal gold particles. J. Bacteriol. 162(3):1250–1254

    Google Scholar 

  • Krafft C, Knetschke T, Funk RH and Salzer R (2006) Studies on stress-induced changes at the subcellular level by Raman microspectroscopic mapping. Anal. Chem. 78(13):4424–4429

    Article  Google Scholar 

  • Lin FY, Sabri M, Alirezaie J, Li D and Sherman PM (2005) Development of a nanoparticle-labeled microfluidic immunoassay for detection of pathogenic microorganisms. Clin. Diagn. Lab. Immunol. 12(3):418–425

    Article  Google Scholar 

  • Litwin CM and Calderwood SB (1994) Analysis of the complexity of gene regulation by fur in Vibrio cholerae. J. Bacteriol. 176(1):240–248

    Google Scholar 

  • Lynch M, Mosher C, Huff J, Nettikadan S, Johnson J and Henderson E (2004) Functional protein nanoarrays for biomarker profiling. Proteomics 4(6):1695–1702

    Article  Google Scholar 

  • Maquelin K, Choo-Smith L-P, van Vreeswijk T, Endtz HP, Smith B, Bennett R, Bruining HA, and Puppels GJ (2000) Raman spectroscopic method for identification of clinically relevant microorganisms growing on solid culture medium. Anal. Chem. 72:12–19

    Article  Google Scholar 

  • Maquelin K, Choo-Smith LP, Endtz HP, Bruining HA and Puppels GJ (2002a) Rapid identification of Candida species by confocal Raman microspectroscopy. J. Clin. Microbiol. 40(2):594–600

    Article  Google Scholar 

  • Maquelin K, Kirschner C, Choo-Smith LP, van den Braak N, Endtz HP, Naumann D and Puppels GJ (2002b) Identification of medically relevant microorganisms by vibrational spectroscopy. J. Microbiol. Methods 51(3):255–271

    Google Scholar 

  • Maquelin K, Kirschner C, Choo-Smith LP, Ngo-Thi NA, van Vreeswijk T, Stammler M, Endtz HP, Bruining HA, Naumann D and Puppels GJ (2003) Prospective study of the performance of vibrational spectroscopies for rapid identification of bacterial and fungal pathogens recovered from blood cultures. J. Clin. Microbiol. 41(1):324–329

    Article  Google Scholar 

  • Maquelin K, Dijkshoorn L, van der Reijdenm TJ and Puppels GJ (2006) Rapid epidemiological analysis of Acinetobacter strains by Raman spectroscopy. J. Microbiol. Methods 64(1):126–131

    Article  Google Scholar 

  • Maresca B (1995) Unraveling the secrets of Histoplasma capsulatum. A model to study morphogenic adaptation during parasite host/host interaction. Verh. K. Acad. Geneeskd. Belg. 57(2):133–156

    Google Scholar 

  • Marquis RE and Bender GR (1985) Mineralisation and heat resistance of bacterial spores. J. Bacteriol. 161:789–791

    Google Scholar 

  • Mastronicolis SK, Boura A, Karaliota A, Magiatis P, Arvanitis N, Litos C, Tsakirakis A, Paraskevas P, Moustaka H and Heropoulos G (2006) Effect of cold temperature on the composition of different lipid classes of the foodborne pathogen Listeria monocytogenes: focus on neutral lipids. Food Microbiol. 23(2):184–194

    Article  Google Scholar 

  • Mauchline WS, Araujo R, Wait R, Dowsett AB, Dennis PJ and Keevil CW (1992) Physiology and morphology of Legionella pneumophila in continuous culture at low oxygen concentration. J. Gen. Microbiol. 138(Pt 11):2371–2380

    Google Scholar 

  • Mauchline WS, James BW, Fitzgeorge RB, Dennis PJ and Keevil CW (1994) Growth temperature reversibly modulates the virulence of Legionella pneumophila. Infect. Immun. 62(7):2995–2997

    Google Scholar 

  • McLauchlin J (1997) The identification of Listeria species. Int. J. Food Microbiol. 38(1):77–81

    Article  Google Scholar 

  • Miller VL and Mekalanos JJ (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170(6):2575–2583

    Google Scholar 

  • Mulvaney SP and Keating CD (2000) Raman spectroscopy. Anal. Chem. 72:145R–157R

    Article  Google Scholar 

  • Naumann D, Helm D and Labischinski H (1991) Microbiological characterizations by FT-IR spectroscopy. Nature 351:81–82

    Article  Google Scholar 

  • Naumann D, Helm D, Labischinski H and Giesbrecht P (1991) The characterization of microorganisms by Fourier-transform infrared spectroscopy (FT-IR). In: Nelsen WH (ed) Modern Techniques for Rapid Microbiological Analysis. VCH, New York, pp 43–96

    Google Scholar 

  • Naumann D, Keller S, Helm D, Schultz NC and Schrader B (1995) FT-IR spectroscopy and Raman spectroscopy are powerful analytical tools for the non-invasive characterization of intact microbial cells. J. Mol. Struct. 347:399–406

    Article  Google Scholar 

  • Ni J, Lipert RJ, Dawson GB and Porter MD (1999) Immunoassay readout method using extrinsic Raman labels adsorbed on immunogold colloids. Anal. Chem. 71(21):4903–4908

    Article  Google Scholar 

  • Nie S and Emory SR (1997) Probing single molecules and single nanoparticles by surface-enhanced Raman scattering. Science 275:1102

    Article  Google Scholar 

  • Notermans S, Dufrenne J, Teunis P and Chackraborty T (1998) Studies on the risk assessment of Listeria monocytogenes. J. Food Prot. 61(2):244–248

    Google Scholar 

  • Notingher I, Selvakumaran J and Hench LL (2004) New detection system for toxic agents based on continuous spectroscopic monitoring of living cells. Biosens. Bioelectron. 20(4):780–789

    Article  Google Scholar 

  • Oliver JD (2005) The viable but nonculturable state in bacteria. J. Microbiol. 43(5):93–100

    Google Scholar 

  • Oust A, Moretro T, Naterstad K, Sockalingum GD, Adt I, Manfait M and Kohler A (2006) Fourier transform infrared and Raman spectroscopy for characterization of Listeria monocytogenes strains. Appl. Environ. Microbiol. 72(1):228–232

    Article  Google Scholar 

  • Palop A, Mañas P and Condón S (1999) Sporulation temperature and heat resistance of Bacillus spores: a review. J. Food Safety 19:57–72

    Article  Google Scholar 

  • Palop A, Sala FJ and Condón S (1999) Heat resistance of native and demineralised spores of Bacillus subtilis sporulated at different temperatures. Appl. Environ. Microbiol. 65:1316–1319

    Google Scholar 

  • Peel M, Donachie W and Shaw A (1988) Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting. J. Gen. Microbiol. 134(Pt 8):2171–2178

    Google Scholar 

  • Perney NMB, Baumberg JJ, Zoorob ME, Charlton MDB, Mahnkopf S and Netti CM (2006) Tuning localized plasmons in nanostructured substrates for surface-enhanced Raman scattering. Opt. Express 14(2):847–857

    Article  Google Scholar 

  • Pettersson,A, Poolman JT, van der Ley P and Tommassen J (1997) Response of Neisseria meningitidis to iron limitation. Antonie Van Leeuwenhoek 71(1–2):129–136

    Article  Google Scholar 

  • Picard-Bonnaud F, Cottin J and Carbonnelle B (1989) Preservation of the virulence of Listeria monocytogenes in different sorts of soil. Acta Microbiol. Hung. 36(2–3):269–272

    Google Scholar 

  • Preisner O, Lopes JA, Guiomar R, Machado J and Menezes JC (2007) Fourier transform infrared (FT-IR) spectroscopy in bacteriology: towards a reference method for bacteria discrimination. Anal. Bioanal. Chem. 387:1733–1748

    Article  Google Scholar 

  • Premasiri WR, Moir DT and Ziegler LD (2005) Vibrational fingerprinting of bacterial pathogens by surface enhanced Raman scattering (SERS). Proc. SPIE 5795:19–29

    Article  Google Scholar 

  • Premasiri WR, Moir DT, Klempner MS, Krieger N, Jones G 2nd and Ziegler LD (2005) Characterization of the surface enhanced Raman scattering (SERS) of bacteria. J. Phys. Chem. B 109(1):312–320

    Article  Google Scholar 

  • Raso J, Barbosa-Cánovas G and Swanson BG (1998) Sporulation temperature affects initiation of germination and inactivation by high hydrostatic pressure of Bacillus cereus. J. Appl. Microbiol. 85:17–24

    Article  Google Scholar 

  • Rösch P, Harz M, Schmitt M, Peschke KD, Ronneberger O, Burkhardt H, Motzkus HW, Lankers M, Hofer S, Thiele H and Popp J (2005) Chemotaxonomic identification of single bacteria by micro-Raman spectroscopy: application to clean-room-relevant biological contaminations. Appl. Environ. Microbiol. 71(3):1626–1637

    Article  Google Scholar 

  • Rösch P, Harz M, Peschke KD, Ronneberger O, Burkhardt H and Popp J (2006) Identification of single eukaryotic cells with micro-Raman spectroscopy. Biopolymers 82(4):312–316

    Article  Google Scholar 

  • Samoilova SV, Samoilova LV, Yezhov IN, Drozdov IG and Anisimov AP (1996) Virulence of pPst+ and pPst- strains of Yersinia pestis for guinea-pigs. J. Med. Microbiol. 45(6):440–444

    Article  Google Scholar 

  • Schuster KC, Urlaub E and Gapes JR (2000) Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and on the heterogeneity in a culture. J. Microbiol. Methods 42:29–38

    Article  Google Scholar 

  • Scott IR and Ellar DJ (1978) Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate. J. Bacteriol. 135(1):133–137

    Google Scholar 

  • Seltmann G, Voigt W and Beer W (1994) Application of physico-chemical typing methods for the epidemiological analysis of Salmonella enteritidis strains of phage type 25/17. Epidemiol. Infect. 113(3):411–424

    Article  Google Scholar 

  • Sengupta A, Laucks ML and Davis EJ (2005) Surface-enhanced Raman spectroscopy of bacteria and pollen. Appl. Spectrosc. 59(8):1016–1023

    Article  Google Scholar 

  • Shibata H, Yamashita S, Ohe M and Tani I (1986) Laser Raman spectroscopy of lyophilized bacterial spores. Microbiol. Immunol. 30(4):307–313

    Google Scholar 

  • Singh GP, Creely CM, Volpe G, Grötsch H and Petrov D (2005) Real-time detection of hyperosmotic stress resonse in optically trapped single yeast cells using Raman microspectroscopy. Anal. Chem. 77:2564–2568

    Article  Google Scholar 

  • Singh GP, Volpe G, Creely CM, Grötsch H, Geli IM and Petrov D (2006) The lag phase and G1 phase of a single yeast cell monitored by Raman microspectroscopy. J. Raman Spectrosc. 37:858–864

    Article  Google Scholar 

  • Sockalingum GD, Lamfarraj H, Beljebbar A, Pina P, Delavenne D, Witthuhn F, Allouch P and Manfait M (1999) Vibrational spectroscopy as a probe to rapidly detect, identify, and characterize micro-organisms. Proc. SPIE 3608:185–194

    Article  Google Scholar 

  • Spencer KM, Sylvia JM, Clauson SL and Janni JA (2002) Surface-enhanced Raman as a water monitor for warfare agents. Proc. SPIE 4577:158–165

    Article  Google Scholar 

  • Stephens JC, Roberts IS, Jones D and Andrew PW (1991) Effect of growth temperature on virulence of strains of Listeria monocytogenes in the mouse: evidence for a dose dependence. J. Appl. Bacteriol. 70(3):239–244

    Google Scholar 

  • Stevenson HJR and Bolduan OE (1952) Infrared spectrophotometry as a means for identification of bacteria. Science 116:111–113

    Article  Google Scholar 

  • Thomas LC and Greenstreet JES (1954) The identification of micro-organisms by infrared spectrophotometry. Spectrochim. Acta 6:302–319

    Article  Google Scholar 

  • Thompson PA, Guan Y, Wood LL and Grow AE (2000a) SBIR Phase I Final Report, Contract DAAD16-00-C-9217. U.S. Army Soldier & Biological Chemical Command

    Google Scholar 

  • Thompson PA, Guan Y, Wood LL and Grow AE (2000b) STTR Phase II Final Report, Contract No. DAAG55-98-C-0004. U.S. Army Research Office

    Google Scholar 

  • Thuan BP, Calderon de la Barca AM, Buck G, Galsworthy SB and Doyle RJ (2000) Interactions between listeriae and lectins. Roum. Arch. Microbiol. Immunol. 59(1–2):55–61

    Google Scholar 

  • Wagner JS, Trahan MW, Nelson WE, Tisone GC and Prepernau BL (1996) How intelligent chemical recognition benefits from multivariate analysis and genetic optimization. Computers in Physics 10(2):113–118

    Google Scholar 

  • Williams OB and Robertson WJ (1954) Studies on heat resistance. VI. Effect of temperature of incubation at which formed on heat resistance of aerobic thermophilic spores. J. Bacteriol. 67:377–378

    Google Scholar 

  • Xie C, Chen D and Li YQ (2005) Raman sorting and identification of single living micro-organisms with optical tweezers. Opt. Lett. 30(14):1800–1802

    Article  Google Scholar 

  • Xie C, Mace J, Dinno MA, Li YQ, Tang W, Newton RJ and Gemperline PJ (2005) Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy. Anal. Chem. 77(14):4390–4397

    Article  Google Scholar 

  • Xu H, Bjerneld EJ, Kall M and Borjesson L (1999) Spectroscopy of single hemoglobin molecules by surface enhanced Raman scattering. Phys. Rev. Lett. 83(21):4357–4360

    Article  Google Scholar 

  • Xu S, Ji X, Xu W, Li X, Wang L, Bai Y, Zhao B and Ozaki Y (2004) Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering. Analyst 129(1):63–68

    Article  Google Scholar 

  • Xu S, Ji X, Xu X, Zhao B, Dou X, Bai Y and Osaki Y (2005) Surface-enhanced Raman scattering studies on immunoassay. J. Biomed. Opt. 10(3):031112-1- 031112-12

    Article  Google Scholar 

  • Zeiri L, Bronk BV, Shabtai Y, Eichler J and Efrima S (2004) Surface-enhanced Raman spectroscopy as a tool for probing specific biochemical components in bacteria. Appl. Spectrosc. 58(1):33–40

    Article  Google Scholar 

  • Zeiri L and Efrima S (2005) Surface-enhanced Raman spectroscopy of bacteria: the effect of excitation wavelength and chemical modification of the colloidal milieu. J. Raman Spectrosc. 36(6–7):667–675

    Article  Google Scholar 

  • Zhu Q, Quivey RG and Berger AJ (2004) Measurement of bacterial concentration fractions in polymicrobial mixtures by Raman microspectroscopy. J. Biomed. Opt. 9(6):1182–1186

    Article  Google Scholar 

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Authors and Affiliations

  1. Biopraxis Inc., San Diego, California

    Ann E. Grow

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  1. Ann E. Grow
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  1. Biophage Pharma Inc, Montreal, Canada

    Mohammed Zourob

  2. Consultant to Biophage Pharma Inc, Montreal, Canada

    Souna Elwary

  3. Cranfield University, Bedfordshire, UK

    Anthony Turner

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© 2008 Springer Science+Business Media, LLC

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Grow, A.E. (2008). Label-Free Fingerprinting of Pathogens by Raman Spectroscopy Techniques. In: Zourob, M., Elwary, S., Turner, A. (eds) Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-75113-9_20

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  • DOI: https://doi.org/10.1007/978-0-387-75113-9_20

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