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Membranes and Fluorescence Microscopy

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Reviews in Fluorescence 2007

Part of the book series: Reviews in Fluorescence ((RFLU,volume 2007))

Abstract

Fluorescence spectroscopy-based techniques using conventional fluorimeters have been extensively applied since the late 1960s to study different aspects of membrane-related phenomena, i.e., mainly relating to lipid-lipid and lipid-protein (peptide) interactions. Even though fluorescence spectroscopy approaches provide very valuable structurally and dynamically related information on membranes, they generally produce mean parameters from data collected on bulk solutions of many vesicles and lack direct information on the spatial organization at the level of single membranes, a quality that can be provided by microscopy-related techniques. In this chapter, I will attempt to summarize representative examples concerning how microscopy (which provides information on membrane lateral organization by direct visualization) and spectroscopy techniques (which provides information about molecular interaction, order and microenvironment) can be combined to give a powerful new approach to study membrane-related phenomena. Additionally along this chapter, it will be discussed how membrane model systems can be further utilized to gain information about particular membrane-related process like protein(peptide)/membrane interactions.

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  1. Membrane Biophysics and Biophotonics Group/MEMPHYS – Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55 DK-5230, Odense M, Denmark

    Luis A. Bagatolli

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Bagatolli, L.A. (2009). Membranes and Fluorescence Microscopy. In: Reviews in Fluorescence 2007. Reviews in Fluorescence, vol 2007. Springer, New York, NY. https://doi.org/10.1007/978-0-387-88722-7_2

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