Abstract
Numerous procedures for the determination of levels of nicotine in body fluids have been developed in recent years, all of which have certain disadvantages. Radioimmunoassay and related procedures have proved to have unique advantages, however, with small samples and in circumstances of low nicotine concentration, 2- and 6-aminonicotine have been found not to be suitable functionalized nicotine haptens for various reasons. In the present study on the use of racemic aminonicotine as a functionalized hapten, nicotine antibodies suitable for use in nicotine determinations have been produced from antigens in which both ‘flexible’ and ‘semi-rigid’ chains serve to couple racemic 6-aminonicotine to bovine serum albumin.
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© 1988 S. B. Pal and the Contributors
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Castro, A., Monji, N. (1988). Nicotine Enzyme Immunoassay. In: Pal, S.B. (eds) Reviews on Immunoassay Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-09854-5_5
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DOI: https://doi.org/10.1007/978-1-349-09854-5_5
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