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Structural and functional studies of ribonuclease T1

  • Chapter
Protein-Nucleic Acid Interaction

Part of the book series: Topics in Molecular and Structural Biology ((TMSB))

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Abstract

Ribonuclease (RNase) T1 represents a simple, yet most rewarding model for studying protein-nucleic acid interaction. The isolation of the enzyme from Takadiastase, a commercial preparation of the culture medium of the mould fungus Aspergillus oryzae, was first described by Sato and Egami (1957). Since then, RNase T1 has received much attention as a tool in molecular biology, notably for RNA sequencing (Donis-Keller et al., 1977; Simoncsits et al., 1911; Silberklang et al., 1979) and mapping (e.g. Epstein et al., 1981; Nohga et al., 1981; Nomoto et al., 1981; Stackebrandt et al., 1981; Stewart and Crouch, 1981). RNase T1 has also been used to catalyse the formation of phosphodiester bonds in RNA (Podder, 1970).

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Authors
  1. Udo Heinemann
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  2. Ulrich Hahn
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Editors and Affiliations

  1. Institut für Kristallographie, Freie Universität Berlin, West Germany

    Wolfram Saenger  & Udo Heinemann  & 

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© 1989 The Contributors

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Heinemann, U., Hahn, U. (1989). Structural and functional studies of ribonuclease T1. In: Saenger, W., Heinemann, U. (eds) Protein-Nucleic Acid Interaction. Topics in Molecular and Structural Biology. Palgrave, London. https://doi.org/10.1007/978-1-349-09871-2_6

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