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Extracellular Enzymes and Metabolites Involved in Pathogenesis of Botrytis

  • Chapter
Botrytis: Biology, Pathology and Control

The infection of host plants by Botrytis spp. is mediated by numerous extracellular enzymes and metabolites. Each of these compounds may play a role in different stages of the infection process. Cutinases, lipases and some cell wall-degrading enzymes may facilitate the penetration of the host surface, while toxins, oxalate and reactive oxygen species may contribute to killing of the host cells. Several cell wall-degrading enzymes contribute to the conversion of host tissue into fungal biomass, but also other enzymes, such as laccases and proteases are potentially involved in pathogenesis. The cloning of the corresponding genes in recent years has facilitated studies on gene expression and targeted mutagenesis. This chapter gives an updated overview of the research performed on these secreted enzymes and metabolites and the role they play in pathogenesis.

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8. References

  • Bar-Nun N and Mayer AM (1990) Cucurbitacins protect cucumber tissue against infection by Botrytis cinerea. Phytochemistry 29: 787-792

    Google Scholar 

  • Benito EP, Ten Have A, Van 't Klooster JW and Van Kan JAL (1998) Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea. European Journal of Plant Pathology 104: 207-220

    Google Scholar 

  • Bennett MH, Gallagher MDS, Bestwick CS, Rossiter JT and Mansfield JW (1994) The phytoalexin response of lettuce to challenge by Botrytis cinerea, Bremia lactucae and Pseudomonas syringae pv. phaseolicola. Physiological and Molecular Plant Pathology 44: 321-333

    Google Scholar 

  • Berto P, Belingheri L and Dehorter B (1997) Production and purification of a novel extracellular lipase from Alternaria brassicicola. Biotechnology Letters 19: 533-536

    Google Scholar 

  • Berto P, Comménil P, Belingheri L and Dehorter B (1999) Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves. FEMS Microbiology Letters 180: 183-189

    Google Scholar 

  • Chen HJ, Smith DL, Starrett DA, Zhou DB, Tucker ML, Solomos T and Gross KC (1997) Cloning and characterization of a rhamnogalacturonan hydrolase gene from Botrytis cinerea. Biochemistry and Molecular Biology International 43: 823-838

    Google Scholar 

  • Chilosi G and Magro P (1997) Pectin lyase and polygalacturonase isoenzyme production by Botrytis cinerea during the early stages of infection on different host plants. Journal of Plant Pathology 78: 61-69

    Google Scholar 

  • Clark CA and Lorbeer JW (1976) Comparative histopathology of Botrytis squamosa and B. cinerea on onion leaves. Phytopathology 66: 1279-1289

    Google Scholar 

  • Clement JA, Martin SG, Porter R, Butt TM and Beckett A (1993a) Germination and the role of the extracellular matrix in adhesion of urediniospores of Uromyces viciae-fabae to synthetic surfaces. Mycological Research 97: 585-593

    Google Scholar 

  • Clement JA, Butt TM and Beckett A (1993b) Characterization of the extracellular matrix produced in vitro by urediniospores and sporelings of Uromyces viciae-fabae. Mycological Research 97: 594-602

    Google Scholar 

  • Cole L, Dewey FM and Hawes CR (1996) Infection mechanisms of Botrytis species: Pre-penetration and pre-infection processes of dry and wet conidia. Mycological Research 100: 277-286

    Google Scholar 

  • Cole L, Dewey FM and Hawes CR (1998) Immunocytochemical studies of the infection mechanisms of Botrytis fabae: II. Host cell wall breakdown. New Phytologist 139: 611-622

    Google Scholar 

  • Collmer A and Keen NT (1986) The role of pectic enzymes in plant pathogenesis. Annual Review of Phytopathology 24: 383-409

    Google Scholar 

  • Colmenares AJ, Aleu J, Durán-Patrón R, Collado IG and Hernández-Galán R (2002) The putative role of botrydial and related metabolites in the infection mechanism of Botrytis cinerea. Journal of Chemical Ecology 28: 997-1005

    Google Scholar 

  • Comménil P, Belingheri L, Sancholle M and Dehorter B (1995) Purification and properties of an extracellular lipase from the fungus Botrytis cinerea. Lipids 30: 351-356

    Google Scholar 

  • Comménil P, Brunet L and Audran JC (1997) The development of the grape berry cuticle in relation to susceptibility to bunch rot disease. Journal of Experimental Botany 48: 1599-1607

    Google Scholar 

  • Comménil P, Belingheri L and Dehorter B (1998) Antilipase antibodies prevent infection of tomato leaves by Botrytis cinerea. Physiological and Molecular Plant Pathology 52: 1-14

    Google Scholar 

  • Comménil P, Belingheri L, Bauw G and Dehorter B (1999) Molecular characterization of a lipase induced in Botrytis cinerea by components of grape berry cuticle. Physiological and Molecular Plant Pathology 55: 37-43

    Google Scholar 

  • Cutler HG, Jacyno JM, Harwood JS, Dulik D, Goodrich PD and Roberts RG (1993) Botcinolide: a biologically active natural product from Botrytis cinerea. Bioscience, Biotechnology and Biochemistry 57: 1980-1982

    Google Scholar 

  • De Bary A (1886) Ueber einige Sclerotinien und Sclerotienkrankheiten. Botanische Zeitung 44: 409-426

    Google Scholar 

  • Deighton N, Muckenschnabel I, Colmenares AJ, Collado IG and Williamson B (2001) Botrydial is produced in plant tissues infected by Botrytis cinerea. Phytochemistry 57: 689-692

    Google Scholar 

  • Deising H, Nicholson RL, Haug M, Howard RJ and Mendgen K (1992) Adhesion pad formation and the involvement of cutinase and esterase in the attachment of Uredospores to the host cuticle. Plant Cell 4: 1101-1111

    Google Scholar 

  • De Meyer G and Höfte M (1997) Salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on bean. Phytopathology 87: 588-593

    Google Scholar 

  • Diaz J, Ten Have A and Van Kan JAL (2002) The role of ethylene and wound signaling in resistance of tomato to Botrytis cinerea. Plant Physiology 129: 1341-1351

    Google Scholar 

  • Dickman MB and Mitra A (1992) Arabidopsis thaliana as a model for studying Sclerotinia sclerotiorum pathogenesis. Physiological and Molecular Plant Pathology 41: 255-263

    Google Scholar 

  • Doss RP (1999) Composition and enzymatic activity of the extracellular matrix secreted by germlings of Botrytis cinerea. Applied and Environmental Microbiology 65: 404-408

    Google Scholar 

  • Drawert F and Krefft M (1978) Charakterisierung extrazellulärer proteine und enzyme aus pektinkulturfiltraten von Botrytis cinerea. Phytochemistry 17: 887-890

    Google Scholar 

  • Durán-Patrón R, Hernandez-Galan R and Collado IG (2000) Secobotrytriendiol and related sesqui-terpenoids: new phytotoxic metabolites from Botrytis cinerea. Journal of Natural Products 63: 182-184

    Google Scholar 

  • Dutton MV and Evans CS (1996) Oxalate production by fungi: its role in pathogenicity and ecology in the soil environment. Canadian Journal of Microbiology 42: 881-895

    Google Scholar 

  • Edlich W, Lorenz G, Lyr H, Nega E and Pommer E-H (1989) New aspects on the infection mechanism of Botrytis cinerea Pers. Netherlands Journal of Plant Pathology 95(supplement 1): 53-62

    Google Scholar 

  • Fan CY and Köller W (1998) Diversity of cutinases from plant pathogenic fungi: differential and sequential expression of cutinolytic esterases by Alternaria brassicicola. FEMS Microbiology Letters 158: 33-38

    Google Scholar 

  • Fu J, Prade R and Mort A (2001) Expression and action pattern of Botryotinia fuckeliana (Botrytis cinerea) rhamnogalacturonan hydrolase in Pichia pastoris. Carbohydrate Research 330: 73-81

    Google Scholar 

  • Gentile AC (1954) Carbohydrate metabolism and oxalic acid synthesis by Botrytis cinerea. Plant Physiology 29: 257-261

    Google Scholar 

  • Germeier C, Hedke K and von Tiedemann A (1994) The use of pH-indicators in diagnostic media for acid-producing plant pathogens. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 101: 498-507

    Google Scholar 

  • Godoy G, Steadman JR, Dickman MB and Dam R (1990) Use of mutants to demonstrate the role of oxalic acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiological and Molecular Plant Pathology 37: 179-191

    Google Scholar 

  • Gonen L, Viterbo A, Cantone F, Staples RC and Mayer AM (1996) Effect of cucurbitacins on mRNA coding for laccase in Botrytis cinerea. Phytochemistry 42: 321-324

    Google Scholar 

  • Govrin EM and Levine A (2000) The hypersensitive response facilitates plant infection by the necrotrophic pathogen Botrytis cinerea. Current Biology 10: 751-757

    Google Scholar 

  • Govrin EM and Levine A (2002) Infection of Arabidopsis with a necrotrophic pathogen, Botrytis cinerea, elicits various defence responses but does not induce systemic acquired resistance (SAR). Plant Molecular Biology 48: 267-276

    Google Scholar 

  • Gross KC, Starrett DA and Chen HJ (1995) Rhamnogalacturonase, D-galactosidase, and E-galactosidase: potential roles in fruit softening. Acta Horticulturae No. 398: 121-130

    Google Scholar 

  • Gueguen Y, Chemardin P, Arnaud A and Galzy P (1995) Purification and characterization of an intracellular E-glucosidase from Botrytis cinerea. Enzyme and Microbial Technology 17: 900-906

    Google Scholar 

  • Hancock JG, Millar RL and Lorbeer JW (1964a) Pectolytic and cellulolytic enzymes produced by Botrytis allii, B. cinerea and B. squamosa in vitro and in vivo. Phytopathology 54: 928-931

    Google Scholar 

  • Hancock JG, Millar RL and Lorbeer JW (1964b) Role of pectolytic and cellulolytic enzymes in Botrytis leaf blight of onion. Phytopathology 54: 932-935

    Google Scholar 

  • Heale JB and Sharman S (1977) Induced resistance to Botrytis cinerea in root slices and tissue cultures of carrot (Daucus carota L.). Physiological Plant Pathology 10: 51-61

    Google Scholar 

  • Johnston DJ and Williamson B (1992a) Purification and characterisation of four polygalacturonases from Botrytis cinerea. Mycological Research 96: 343-349

    Google Scholar 

  • Johnston DJ and Williamson B (1992b) An immunological study in the induction of polygalacturonases of Botrytis cinerea. FEMS Microbiology Letters 97: 19-24

    Google Scholar 

  • Kapat A, Zimand G and Elad Y (1998) Biosynthesis of pathogenicity hydrolytic enzymes by Botrytis cinerea during infection of bean leaves and in vitro. Mycological Research 102: 1017-1024

    Google Scholar 

  • Keates SE, Loewus FA, Helms GL and Zink DL (1998) A5-O-(Į-D-galactopyranosyl)-D-glycero-pent-2-enono-1,4-lactone: characterization in the oxalate-producing fungus, Sclerotinia sclerotiorum. Phytochemistry 49: 2397-2401

    Google Scholar 

  • Kerssies A and Frinking HD (1996) Relations between glasshouse climate and dry weight of petals, epicuticular wax, cuticle, pre-harvest flowering period and susceptibility to Botrytis cinerea of gerbera and rose flowers. European Journal of Plant Pathology 102: 257-263

    Google Scholar 

  • Kersten PJ and Kirk TK (1987) Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium. Journal of Bacteriology 169: 2195-2201

    Google Scholar 

  • Köller W, Yao C, Trial F and Parker DM (1995) Role of cutinases in the infection of plants. Canadian Journal of Botany 73 (Supplement 1): 1109-1118

    Google Scholar 

  • Lee TH, Kim BY, Chung YR, Lee SY, Lee CW and Kim JW (1997) Purification and characterization of an exo-polygalacturonase from Botrytis cinerea. Korean Journal of Microbiology 35: 134-140

    Google Scholar 

  • Leone G (1990) In vivo and in vitro phosphate-dependent polygalacturonase production by different isolates of Botrytis cinerea. Mycological Research 94: 1039-1045

    Google Scholar 

  • Leone G, Schoffelmeer EAM and Van den Heuvel J (1990a) Purification and characterization of a constitutive polygalacturonase associated with the infection process of French bean leaves by Botrytis cinerea. Canadian Journal of Botany 68: 1921-1930

    Google Scholar 

  • Leone G, Overkamp AN, Kreyenbroek MN, Smit E and Van den Heuvel J (1990b) Regulation by orthophosphate and adenine nucleotides of the biosynthesis of two polygalacturonases by Botrytis cinerea in vitro. Mycological Research 94: 1031-1038

    Google Scholar 

  • Liu S, Oeljeklaus S, Gerhardt B and Tudzynski B (1998) Purification and characterisation of glucose oxidase of Botrytis cinerea. Physiological and Molecular Plant Pathology 53: 123-132

    Google Scholar 

  • Loewus FA (1999) Biosynthesis and metabolism of ascorbic acid in plants and of analogs of ascorbic acid in fungi. Phytochemistry 52: 193-210

    Google Scholar 

  • Loewus FA, Saito K, Suto RK and Maring E (1995) Conversion of D-arabinose to D-erythroascorbic acid and oxalic acid in Sclerotinia sclerotiorum. Biochemical and Biophysical Research Communications 212: 196-203

    Google Scholar 

  • Mansfield JW and Richardson A (1981) The ultrastructure of interactions between Botrytis species and broad bean leaves. Physiological Plant Pathology 19: 41-48

    Google Scholar 

  • Manteau S, Abouna S, Lambert B and Legendre L (2003) Differential regulation by ambient pH of putative virulence factor secretion by the phytopathogenic fungus Botrytis cinerea. FEMS Microbiology Ecology 43: 359-366

    Google Scholar 

  • Marcus L and Schejter A (1983) Single step chromatographic purification and characterization of the endopolygalacturonases and pectinesterases of the fungus, Botrytis cinerea Pers. Physiological Plant Pathology 22: 1-13

    Google Scholar 

  • Maule AJ and Ride JP (1976) Ammonia-lyase and O-methyl transferase activities related to lignification in wheat leaves infected with Botrytis. Phytochemistry 15: 1661-1664

    Google Scholar 

  • Movahedi S and Heale JB (1990a) Purification and characterization of an aspartic proteinase secreted by Botrytis cinerea Pers. ex. Pers. in culture and in infected carrots. Physiological and Molecular Plant Pathology 36: 289-302

    Google Scholar 

  • Movahedi S and Heale JB (1990b) The roles of aspartic proteinase and endo-pectin lyase enzymes in the primary stages of infection and pathogenesis of various host tissues by different isolates of Botrytis cinerea Pers. ex. Pers. Physiological and Molecular Plant Pathology 36: 303-324

    Google Scholar 

  • Pascholati SF, Yoshioka H, Kunoh H and Nicholson RL (1992) Preparation of the infection court by Erysiphe graminis f.sp. hordei: cutinase is a component of the conidial exudate. Physiological and Molecular Plant Pathology 41: 53-59

    Google Scholar 

  • Pascholati SF, Deising H, Leite B, Anderson D and Nicholson RL (1993) Cutinase and non-specific esterase activities in the conidial mucilage of Colletotrichum graminicola. Physiological and Molecular Plant Pathology 42: 37-51

    Google Scholar 

  • Pashkoulov D, Giannetti I, Benvenuto E and De Martinis, D (2002) Biochemical characterization of polygalacturonases from five different isolates of Botrytis cinerea. Mycological Research 106: 827-831

    Google Scholar 

  • Patykowksi J and Urbanek H (2003) Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea. Journal of Phytopathology 151: 153-161

    Google Scholar 

  • Pedersen H, Hjort C and Nielsen J (2000) Cloning and characterization of oah, the gene encoding oxaloacetate hydrolase in Aspergillus niger. Molecular and General Genetics 263: 281-286

    Google Scholar 

  • Poinssot B, Vandelle E, Bentéjac M, Adrian M, Levis C, Brygoo Y, Garin J, Sicilia F, Coutos-Thévenot P and Pugin A (2003) The endopolygalacturonase 1 from Botrytis cinerea activates grapevine defense reactions unrelated to its enzymatic activity. Molecular Plant-Microbe Interactions 16: 553-564

    Google Scholar 

  • Prins TW, Wagemakers L, Schouten A and Van Kan JAL (2000a) Cloning and characterization of a glutathione S-transferase homologue from the plant pathogenic fungus Botrytis cinerea. Molecular Plant Pathology 1: 169-178

    Google Scholar 

  • Prins TW, Tudzynski P, Von Tiedemann A, Tudzynski B, Ten Have A, Hansen ME, Tenberge K and Van Kan JAL (2000b) Infection strategies of Botrytis cinerea and related necrotrophic pathogens. In: Kronstad JW (ed.) Fungal Pathology. (pp. 33-64) Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Rebordinos L, Cantoral JM, Prieto MV, Hanson JR and Collado IG (1996) The phytotoxic activity of some metabolites of Botrytis cinerea. Phytochemistry 42: 383-387

    Google Scholar 

  • Reignault P, Mercier M, Bompeix G and Boccara M (1994) Pectin methylesterase from Botrytis cinerea: physiological, biochemical and immunochemical studies. Microbiology 140: 3249-3255

    Google Scholar 

  • Reignault P, Kunz C, Delage N, Moreau E, Vedel R, Hamada W, Bompeix G and Boccara M (2000) Host- and symptom-specific pectinase isozymes produced by Botrytis cinerea. Mycological Research 104: 421-428

    Google Scholar 

  • Rha E, Park HJ, Kim MO, Chung YR, Lee CW and Kim JW (2001) Expression of exo-polygalacturonases in Botrytis cinerea. FEMS Microbiology Letters 201: 105-109

    Google Scholar 

  • Rogers LM, Flaishman MA and Kolattukudy PE (1994) Cutinase gene disruption in Fusarium solani f sp. pisi decreases its virulence on pea. Plant Cell 6: 935-945

    Google Scholar 

  • Rolke Y, Liu S, Quidde T, Williamson B, Schouten A, Weltring K-M, Siewers V, Tenberge KB, Tudzynski B and Tudzynski P (2004) Functional analysis of H2O2-generating systems in Botrytis cinerea: the major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable. Molecular Plant Pathology 5: 17-27

    Google Scholar 

  • Rollins JA (2003) The Sclerotinia sclerotiorum pac1 gene is required for sclerotial development and virulence. Molecular Plant-Microbe Interactions 16: 785-795

    Google Scholar 

  • Salinas J (1992) Function of cutinolytic enzymes in the infection of gerbera flowers by Botrytis cinerea. Ph.D. Thesis, University of Utrecht, The Netherlands

    Google Scholar 

  • Salinas J and Verhoeff K (1995) Microscopical studies of the infection of gerbera flowers by Botrytis cinerea. European Journal of Plant Pathology 101: 377-386

    Google Scholar 

  • Sasaki I and Nagayama H (1994) E-Glucosidase from Botrytis cinerea: Its relation to the pathogenicity of this fungus. Bioscience, Biotechnology and Biochemistry 58: 616-620

    Google Scholar 

  • Schaller A and Ryan CA (1996) Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein. Plant Molecular Biology 31: 1073-1077

    Google Scholar 

  • Schouten A, Tenberge KB, Vermeer J, Stewart J, Wagemakers L, Williamson B and Van Kan JAL (2002a) Functional analysis of an extracellular catalase of Botrytis cinerea. Molecular Plant Pathology 3: 227-238

    Google Scholar 

  • Schouten A, Wagemakers L, Stefanato FL, Van der Kaaij RM and Van Kan JAL (2002b) Resveratrol acts as a natural profungicide and induces self-intoxication by a specific laccase. Molecular Microbiology 43: 883-894

    Google Scholar 

  • Schulze Gronover C, Kasulke D, Tudzynski P and Tudzynski B (2001) The role of G protein alpha subunits in the infection process of the gray mold fungus Botrytis cinerea. Molecular Plant-Microbe Interactions 14: 1293-1302

    Google Scholar 

  • Stahl DJ and Schäfer W (1992) Cutinase is not required for fungal pathogenicity on pea. Plant Cell 4: 621-629

    Google Scholar 

  • Stahl DJ, Theuerkauf A, Heitefuss R and Schäfer W (1994) Cutinase of Nectria haematococca (Fusarium solani f.sp. pisi) is not required for fungal virulence or organ specificity. Molecular Plant-Microbe Interactions 7: 713-725

    Google Scholar 

  • Staples RC and Mayer AM (1995) Putative virulence factors of Botrytis cinerea acting as a wound pathogen. FEMS Microbiology Letters 134: 1-7

    Google Scholar 

  • Sutton JC, Rowell PM and James TDW (1984) Effects of leaf wax, wetness duration and temperature on infection of onion leaves by Botrytis squamosa. Phytoprotection 65: 65-68

    Google Scholar 

  • Ten Have A, Mulder W, Visser J and Van Kan JAL (1998) The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Molecular Plant-Microbe Interactions 11: 1009-1016

    Google Scholar 

  • Ten Have A, Oude Breuil W, Wubben JP, Visser J and Van Kan JAL (2001) Botrytis cinerea endopolygalacturonase genes are differentially expressed in various plant tissues. Fungal Genetics and Biology 33: 97-105

    Google Scholar 

  • Ten Have A, Tenberge KB, Benen JAE, Tudzynski P, Visser J and Van Kan JAL (2002) The contribution of the cell wall degrading enzymes to pathogenesis of fungal plant pathogens. In: Kempken (ed.) The Mycota, A comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research. XI. Agricultural Applications (pp. 341-358) Springer-Verlag, Berlin, Heidelberg, Germany

    Google Scholar 

  • Ten Have A, Dekkers E, Kay J, Phylip LH and Van Kan JAL (2004) An aspartic proteinase gene family in the filamentous fungus Botrytis cinerea contains members with novel features. Microbiology 150: 2475-2489

    Google Scholar 

  • Tobias RB, Conway W and Sams C (1993) Polygalacturonase isozymes from Botrytis cinerea grown on apple pectin. Biochemistry and Molecular Biology International 30: 829-837

    Google Scholar 

  • Tobias RB, Conway WS and Sams CE (1995) Polygalacturonase produced in apple tissue decayed by Botrytis cinerea. Biochemistry and Molecular Biology International 35: 813-823

    Google Scholar 

  • Tör M, Yemm A and Holub E (2003) The role of proteolysis in R gene mediated defence in plants. Molecular Plant Pathology 4: 287-296

    Google Scholar 

  • Urbanek H and Zalewska-Sobczak J (1984) Multiplicity of cell wall degrading glycosidic hydrolases produced by apple infecting Botrytis cinerea. Phytopathologische Zeitschrift 110: 261-271

    Google Scholar 

  • Valette-Collet O, Cimerman A, Reignault P, Levis C and Boccara M (2003) Disruption of Botrytis cinerea pectin methylesterase gene Bcpme1 reduces virulence on several host plants. Molecular Plant-Microbe Interactions 16: 360-367

    Google Scholar 

  • Van den Heuvel J (1981) Effect of inoculum composition on infection of French bean leaves by conidia of Botrytis cinerea. Netherlands Journal of Plant Pathology 87: 55-64

    Google Scholar 

  • Van den Heuvel J and Waterreus LP (1985) Pectic enzymes associated with phosphate-stimulated infection of French bean leaves by Botrytis cinerea. Netherlands Journal of Plant Pathology 91: 253-264

    Google Scholar 

  • Van der Cruyssen G, De Meester E and Kamoen O (1994) Expression of polygalacturonases of Botrytis cinerea in vitro and in vivo. Mededelingen Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen Universiteit Gent 59: 895-905

    Google Scholar 

  • Van der Vlugt-Bergmans CJB, Wagemakers CAM and Van Kan JAL (1997) Cloning and expression of the cutinase A gene of Botrytis cinerea. Molecular Plant-Microbe Interactions 10: 21-29

    Google Scholar 

  • Van Kan JAL, Van't Klooster JW, Wagemakers CAM, Dees DCT and Van der Vlugt-Bergmans CJB (1997) Cutinase A of Botrytis cinerea is expressed, but not essential, during penetration of gerbera and tomato. Molecular Plant-Microbe Interactions 10: 30-38

    Google Scholar 

  • Verhoeff K and Warren JM (1972) In vitro and in vivo production of cell wall degrading enzymes by Botrytis cinerea from tomato. Netherlands Journal of Plant Pathology 78: 179-185

    Google Scholar 

  • Verhoeff K, Leeman M, Van Peer R, Posthuma L, Schot N and Van Eijk GW (1988) Changes in pH and the production of organic acids during colonisation of tomato petioles by Botrytis cinerea. Journal of Phytopathology 122: 327-336

    Google Scholar 

  • Viterbo A, Yagen B and Mayer AM (1993a) Cucurbitacins, 'attack' enzymes and laccase in Botrytis cinerea. Phytochemistry 32: 61-65

    Google Scholar 

  • Viterbo A, Yagen B, Rosenthal R and Mayer AM (1993b) Dependence of activity of cucurbitacin in repression of Botrytis laccase on its structure. Phytochemistry 33: 1313-1315

    Google Scholar 

  • Viterbo A, Staples RC, Yagen B and Mayer AM (1994) Selective mode of action of cucurbitacin in the inhibition of laccase formation in Botrytis cinerea. Phytochemistry 35: 1137-1142

    Google Scholar 

  • Von Tiedemann A (1997) Evidence for a primary role of active oxygen species in induction of host cell death during infection of leaves with Botrytis cinerea. Physiological and Molecular Plant Pathology 50: 151-166

    Google Scholar 

  • Walton JD (1996) Host selective toxins: agents of compatibility. Plant Cell 8: 1723-1733

    Google Scholar 

  • Williamson B, Duncan GH, Harrison JG, Harding LA, Elad Y and Zimand G (1995) Effect of humidity on infection of rose petals by dry-inoculated conidia of Botrytis cinerea. Mycological Research 99: 1303-1310

    Google Scholar 

  • Wubben JP, Mulder W, Ten Have A, Van Kan JAL and Visser J (1999) Cloning and partial characterization of endopolygalacturonase genes from Botrytis cinerea. Applied and Environmental Microbiology 65: 1596-1602

    Google Scholar 

  • Wubben JP, Ten Have A, Van Kan JAL and Visser J (2000) Regulation of endopolygalacturonase gene expression in Botrytis cinerea by galacturonic acid, ambient pH and carbon catabolite repression. Current Genetics 37:152-157

    Google Scholar 

  • Yao C and Köller W (1995) Diversity of cutinases from plant pathogenic fungi: different cutinases are expressed during saprophytic and pathogenic stages of Alternaria brassicicola. Molecular Plant-Microbe Interactions 8: 122-130

    Google Scholar 

  • Zimand G, Elad Y and Chet I (1996) Effect of Trichoderma harzianum on Botrytis cinerea pathogenicity. Phytopathology 86: 1255-1260

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Phytopathology, Wageningen University Plant Sciences, Binnenhaven 5, 6709 PD, Wageningen, Netherlands

    Ilona Kars & Jan A. L. van Kan

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  1. Ilona Kars
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  2. Jan A. L. van Kan
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  1. The Volcani Center, 50250, Bet Dagan, Israel

    Yigal Elad

  2. Invergowrie, DD2 5DA, Dundee, UK

    Brian Williamson

  3. Westfälische Wilhelms-Universität, Schlossgarten 3, D-48149, Münster, Germany

    Paul Tudzynski

  4. Ege University, Bornova, Izmir, Turkey

    Nafiz Delen

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Kars, I., van Kan, J.A.L. (2007). Extracellular Enzymes and Metabolites Involved in Pathogenesis of Botrytis. In: Elad, Y., Williamson, B., Tudzynski, P., Delen, N. (eds) Botrytis: Biology, Pathology and Control. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2626-3_7

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