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Molecular Interactions of the Stromal Subunit PsaC with the PsaA/PsaB Heterodimer

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Photosystem I

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 24))

Abstract

The X-ray crystal structure of cyanobacterial Photosystem I (PS I) has been solved to atomic resolution (PDB entry 1JB0). It provides a structural model for the subunits PsaC, PsaD, and PsaE that comprise the stromal ridge of PS I. Independently, the three-dimensional solution structures of unbound, recombinant PsaC (PDB entry 1K0T) and PsaE (PDB entries 1PSF, 1QP2, and 1GXI) have been solved using NMR spectroscopy.

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References

  • Adman ET, Sieker LC and Jensen LH (1973) The structure of a bacterial ferredoxin. J Biol Chem 248: 3987–3996

    PubMed  CAS  Google Scholar 

  • Adman ET, Siefker LC and Jensen LH (1976) Structure of Peptococcus aerogenes ferredoxin. Refinement at 2 Å resolution. J Biol Chem 251: 3801–3806

    PubMed  CAS  Google Scholar 

  • Antonkine ML, Bentrop D, Bertini I, Luchinat C, Shen GZ, Bryant DA, Stehlik D and Golbeck JH (2000) Paramagnetic 1H NMR spectroscopy of the reduced, unbound Photosystem I subunit PsaC: sequence-specific assignment of contact-shifted resonances and identification of mixed- and equal-valence Fe-Fe pairs in [4Fe-4S] centers FA and FB . J Biol Inorg Chem 5: 381–392

    Article  PubMed  CAS  Google Scholar 

  • Antonkine ML, Liu GH, Bentrop D, Bryant DA, Bertini I, Luchinat C, Golbeck JH and Stehlik D (2002) Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I. J Biol Inorg Chem 7: 461–472

    Article  PubMed  CAS  Google Scholar 

  • Antonkine ML, Jordan P, Fromme P, Krauß N, Golbeck JH and Stehlik D (2003) Assembly of protein subunits within the stromal ridge of Photosystem I. Structural changes between unbound and sequentially PS I-bound polypeptides and correlated changes of the magnetic properties of the terminal iron sulfur clusters. J Mol Biol 327: 671–697

    Article  PubMed  CAS  Google Scholar 

  • Aono S, Bentrop D, Bertini I, Cosenza G and Luchinat C (1998a) Solution structure of an artificial Fe8S8 ferredoxin: the D13C variant of Bacillus schlegelii Fe7S8 ferredoxin. Eur J Biochem 258: 502–514

    Article  CAS  Google Scholar 

  • Aono S, Bentrop D, Bertini I, Donaire A, Luchinat C, Niikura Y and Rosato A (1998b) Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium Bacillus schlegelii by 1H NMR spectroscopy. Biochemistry 37: 9812–9826

    Article  CAS  Google Scholar 

  • Barth P, Savarin P, Gilquin B, Lagoutte B and Ochsenbein F (2002) Solution NMR structure and backbone dynamics of the PsaE subunit of Photosystem I from Synechocystis sp. PCC 6803. Biochemistry 41: 13902–13914

    Article  PubMed  CAS  Google Scholar 

  • Ben-Shem A, Frolow F and Nelson N (2003) Crystal structure of plant Photosystem I. Nature 426: 630–635

    Article  PubMed  CAS  Google Scholar 

  • Bentrop D, Bertini I, Luchinat C, Nitschke W and Mühlenhoff U (1997) Characterization of the unbound 2[Fe4S4] ferredoxin-like Photosystem I subunit PsaC from the cyanobacterium Synechococcus elongatus. Biochemistry 36: 13629–13637

    Article  PubMed  CAS  Google Scholar 

  • Bentrop D, Capozzi F and Luchinat C (2001) Iron-sulfur proteins. In: Bertini I, Sigel A and Sigel H (eds) Handbook on Metalloproteins, pp 357–460. Marcel Dekker, New York

    Google Scholar 

  • Bertini I, Donaire A, Feinberg BA, Luchinat C, Piccioli M and Yuan HP (1995) Solution structure of the oxidized 2[4Fe-4S] ferredoxin from Clostridium pasteurianum. Eur J Biochem 232: 192–205

    Article  PubMed  CAS  Google Scholar 

  • Cantrell A and Bryant DA (1987) Molecular-cloning and nucleotide-sequence of the psaA and psaB genes of the cyanobacterium Synechococcus sp. PCC 7002. Plant Mol Biol 9: 453–468

    Article  CAS  Google Scholar 

  • Chamorovsky SK and Cammack R (1982) Direct determination of the midpoint potential of the acceptor X in chloroplast Photosystem I by electrochemical reduction and ESR spectroscopy. Photobiochem Photobiophys 4: 195–200

    CAS  Google Scholar 

  • Chitnis VP, Jung YS, Albee L, Golbeck JH and Chitnis PR (1996) Mutational analysis of Photosystem I polypeptides –role of PsaD and the Lysyl 106 residue in the reductase activity of photosystem I. J Biol Chem 271: 11772–11780

    Article  PubMed  CAS  Google Scholar 

  • Dauter Z, Wilson KS, Sieker LC, Meyer J and Moulis JM (1997) Atomic resolution (0.94 Å) structure of Clostridium acidi urici ferredoxin. Detailed geometry of [4Fe-4S] clusters in a protein. Biochemistry 36: 16065–16073

    Article  PubMed  CAS  Google Scholar 

  • Diaz-Moreno I, Diaz-Quintana A, Molina-Heredia FP, Nieto PM, Hansson O, De la Rosa MA and Karlsson BG (2005) NMR analysis of the transient complex between membrane Photosystem I and soluble cytochrome c6. J Biol Chem 280: 7925–7931

    Article  PubMed  CAS  Google Scholar 

  • Diaz-Quintana A, Leibl W, Bottin H and Sétif P (1998) Electron transfer in Photosystem I reaction centers follows a linear pathway in which iron-sulfur cluster FB is the immediate electron donor to soluble ferredoxin. Biochemistry 37: 3429–3439

    Article  PubMed  CAS  Google Scholar 

  • Duee ED, Fanchon E, Vicat J, Sieker LC, Meyer J and Moulis JM (1994) Refined crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acid urici at 1.84 Å resolution. J Mol Biol 243: 683–695

    Article  PubMed  CAS  Google Scholar 

  • Falzone CJ, Kao YH, Zhao JD, Bryant DA and Lecomte JTJ (1994) Three-dimensional solution structure of PsaE from the cyanobacterium Synechococcus sp. strain PCC 7002, a Photosystem I protein that shows structural homology with SH3 domains. Biochemistry 33: 6052–6062

    Article  PubMed  CAS  Google Scholar 

  • Fiaux J, Bertelsen EB, Horwich AL and Wuthrich K (2002) NMR analysis of a 900K GroEL-GroES complex. Nature 418: 207–211

    Article  PubMed  CAS  Google Scholar 

  • Fischer N, Hippler M, Sétif P, Jacquot JP and Rochaix JD (1998) The PsaC subunit of photosystem I provides an essential lysine residue for fast electron transfer to ferredoxin. EMBO J 17: 849–858

    Article  PubMed  CAS  Google Scholar 

  • Fischer N, Sétif P and Rochaix JD (1999) Site-directed mutagenesis of the PsaC subunit of Photosystem I. FB is the cluster interacting with soluble ferredoxin. J Biol Chem 274: 23333–23340

    Article  PubMed  CAS  Google Scholar 

  • Fish L, Kück U and Bogorad L (1985) Two partially adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a protein complex of Photosystem I. J Biol Chem 260: 1413–1421

    PubMed  CAS  Google Scholar 

  • Fromme P, Jordan P and Krauß N (2001) Structure of Photosystem I. Biochim Biophys Acta 1507: 5–31

    Article  PubMed  CAS  Google Scholar 

  • Golbeck JH (1994) Photosystem I in cyanobacteria. In: Bryant DA (ed) The Molecular Biology of Cyanobacteria, pp 179–220. Kluwer Academic Publishers, The Netherlands

    Google Scholar 

  • Golbeck JH (1999) A comparative analysis of the spin state distribution of in vitro and in vivo mutants of PsaC –a biochemical argument for the sequence of electron transfer in Photosystem I as FX → FA → FB → ferredoxin/flavodoxin. Photosynth Res 61: 107–144

    Article  CAS  Google Scholar 

  • Golbeck JH and Bryant DA (1991) Photosystem I. In: Lee CP (ed) Current Topics in Bioenergetics, Vol 16, pp 83–177. Academic Press, Inc., San Diego

    Google Scholar 

  • Golbeck JH, Parrett KG, Mehari T, Jones KL and Brand JJ (1988) Isolation of the intact Photosystem I reaction center core containing P700 and iron-sulfur center FX. FEBS Lett 228: 268–272

    Article  CAS  Google Scholar 

  • Heinnickel M, Shen G, Agalarov R and Golbeck J (2005) Resolution and reconstitution of a bound Fe-S protein from the photosynthetic reaction center of Heliobacterium modesticaldum. Biochemistry 44: 9950–9960

    Article  PubMed  CAS  Google Scholar 

  • Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauß N (2001) Three-dimensional structure of cyanobacterial Photosystem I at 2.5 Å resolution. Nature 411: 909–917

    Article  PubMed  CAS  Google Scholar 

  • Jung YS, Yu L and Golbeck JH (1995) Reconstitution of iron-sulfur center FB results in complete restoration of NADP+ photoreduction in Hg-treated Photosystem I complexes from Synechococcus sp PCC 6301. Photosynth Res 46: 249–255

    Article  CAS  Google Scholar 

  • Ke B, Dolan E, Sugahara K, Hawkridge FM, Demeter S and Shaw ER (1977) Electrochemical and kinetic evidence for a transient electron acceptor in the photochemical charge separation in Photosystem I. In: Photosynthetic Organelles [Plant Cell Physiol (Special Issue no. 3)] pp 187–199

    Google Scholar 

  • Klukas O, Schubert WD, Jordan P, Krauß N, Fromme P, Witt HT and Saenger W (1999) Photosystem I, an improved model of the stromal subunits PsaC, PsaD, and PsaE. J Biol Chem 274: 7351–7360

    Article  PubMed  CAS  Google Scholar 

  • Koradi R, Billeter M and Wuthrich K (1996) MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph 14: 29–32, 51–55

    Google Scholar 

  • Lakshmi KV, Jung YS, Golbeck JH and Brudvig GW (1999) Location of the iron-sulfur clusters FA and FB in Photosystem I: an electron paramagnetic resonance study of spin relaxation enhancement of P700+. Biochemistry 38: 13210–13215

    Article  PubMed  CAS  Google Scholar 

  • Li N, Zhao J, Warren PV, Warden JT, Bryant DA and Golbeck JH (1991a) PsaD is required for the stable binding of PsaC to the Photosystem I core protein of Synechococcus sp. PCC 6301. Biochemistry 30: 7863–7872

    Article  CAS  Google Scholar 

  • Li N, Warren P, Golbeck J, Frank G, Zuber H and Bryant D (1991b) Polypeptide composition of the Photosystem-I complex and the Photosystem-I core protein from Synechococcus sp. PCC 6301. Biochim Biophys Acta 1059: 215–225

    Article  CAS  Google Scholar 

  • Locher KP, Hans M, Yeh AP, Schmid B, Buckel W and Rees DC (2001) Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A. J Mol Biol 307: 297–308

    Article  PubMed  CAS  Google Scholar 

  • Lovenberg W, Rabinowitz JC and Buchanan BB (1963) Studies on chemical nature of clostridial ferredoxin. J Biol Chem 238: 3899–3913

    PubMed  CAS  Google Scholar 

  • Mamedov MD, Gourovskaya KN, Vassiliev IR, Golbeck JH and Sememov AY (1998) Electrogenicity accompanies photoreduction of the iron-sulfur clusters FA and FB in Photosystem I. FEBS Lett 431: 219–223

    Article  PubMed  CAS  Google Scholar 

  • Mayer KL, Shen GZ, Bryant DA, Lecomte JTJ and Falzone CJ (1999) The solution structure of Photosystem I accessory protein E from the cyanobacterium Nostoc sp. strain PCC 8009. Biochemistry 38: 13736–13746

    Article  PubMed  CAS  Google Scholar 

  • McDermott AE, Yachandra VK, Guiles RD, Sauer K, Klein MP, Parrett KG and Golbeck JH (1989) EXAFS structural study of FX, the low-potential Fe-S center in Photosystem I. Biochemistry 28: 8056–8059

    Article  PubMed  CAS  Google Scholar 

  • Mehari T, Parrett KG, Warren PV and Golbeck JH (1991) Reconstitution of the iron-sulfur clusters in the isolated FA/FB protein: EPR spectral characterization of same-species and cross-species Photosystem I complexes. Biochim Biophys Acta 1056: 139–148

    Article  CAS  Google Scholar 

  • Moulis JM, Sieker LC, Wilson KS and Dauter Z (1996) Crystal structure of the 2[4Fe-4S] ferredoxin from Chromatium vinosum: evolutionary and mechanistic inferences for [3/4Fe-4S] ferredoxins. Protein Sci 5: 1765–1775

    PubMed  CAS  Google Scholar 

  • Parrett KG, Mehari T, Warren PG and Golbeck JH (1989) Purification and properties of the intact P700 and FX-containing Photosystem I core protein. Biochim Biophys Acta 973: 324–332

    PubMed  CAS  Google Scholar 

  • Pervushin K, Riek R, Wider G and Wuthrich K (1997) Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci USA 94: 12366–12371

    Article  PubMed  CAS  Google Scholar 

  • Petrouleas V, Brand JJ, Parrett KG and Golbeck JH (1989) A Mössbauer analysis of the low-potential iron-sulfur center in Photosystem I –spectroscopic evidence that FX is a 4Fe-4S cluster. Biochemistry 28: 8980–8983

    Article  PubMed  CAS  Google Scholar 

  • Rousseau F, Sétif P and Lagoutte B (1993) Evidence for the involvement of PSI-E subunit in the reduction of ferredoxin by Photosystem I. EMBO J 12: 1755–1765

    PubMed  CAS  Google Scholar 

  • Saenger W, Jordan P and Krauß N (2002) The assembly of protein subunits and cofactors in Photosystem I. Curr Opin Struct Biol 12: 244–254

    Article  PubMed  CAS  Google Scholar 

  • Schlessman JL, Woo D, Joshua-Tor L, Howard JB and Rees DC (1998) Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum. J Mol Biol 280: 669–685

    Article  PubMed  CAS  Google Scholar 

  • Sétif P (2001) Ferredoxin and flavodoxin reduction by Photosystem I. Biochim Biophys Acta 1507: 161–179

    Article  PubMed  Google Scholar 

  • Shinkarev VP, Vassiliev IR and Golbeck JH (2000) A kinetic assessment of the sequence of electron transfer from FX to FA and further to FB in Photosystem I: the value of the equilibrium constant between FX and FA. Biophys J 78: 363–372

    PubMed  CAS  Google Scholar 

  • Sticht H and Rosch P (1998) The structure of iron-sulfur proteins. Prof Phys Mol Biol 70: 95–136

    CAS  Google Scholar 

  • Stout CD (1993) Crystal structures of oxidized and reduced Azotobacter vinelandii ferredoxin at pH 8 and pH 6. J Biol Chem 268: 25920–25927

    PubMed  CAS  Google Scholar 

  • Tranqui D and Jesior JC (1995) Structure of the ferredoxin from Clostridium acidi urici –model at 1.8 Å resolution. Acta Crystallogr D Biol Crystallogr 51: 155–159

    Article  PubMed  CAS  Google Scholar 

  • Unciuleac M, Boll M, Warkentin E and Ermler U (2004) Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin. Acta Crystallogr D Biol Crystallogr 60: 388–391

    Article  PubMed  CAS  Google Scholar 

  • Vassiliev IR, Jung YS, Yang F and Golbeck JH (1998) PsaC subunit of Photosystem I is oriented with iron-sulfur cluster FB as the immediate electron donor to ferredoxin and flavodoxin. Biophys J 74: 2029–2035

    Article  PubMed  CAS  Google Scholar 

  • Vassiliev IR, Antonkine ML and Golbeck JH (2001) Iron-sulfur clusters in type I reaction centers. Biochim Biophys Acta 1507: 139–160

    Article  PubMed  CAS  Google Scholar 

  • Xia ZC, Broadhurst RW, Laue ED, Bryant DA, Golbeck JH and Bendall DS (1998) Structure and properties in solution of PsaD, an extrinsic polypeptide of Photosystem I. Eur J Biochem 255: 309–316

    Article  PubMed  CAS  Google Scholar 

  • Xu Q, Jung YS, Chitnis VP, Guikema JA, Golbeck JH and Chitnis PR (1994) Mutational analysis of Photosystem I polypeptides in Synechocystis sp. PCC 6803 –subunit requirements for reduction of NADP+ mediated by ferredoxin and flavodoxin. J Biol Chem 269: 21512–21518

    PubMed  CAS  Google Scholar 

  • Yu JP, Smart LB, Jung YS, Golbeck J and Mcintosh L (1995) Absence of PsaC subunit allows assembly of Photosystem I core but prevents the binding of PsaD and PsaE in Synechocystis sp PCC 6803. Plant Mol Biol 29: 331–342

    Article  PubMed  CAS  Google Scholar 

  • Zhao J, Warren PV, Li N, Bryant DA and Golbeck JH (1990) Reconstitution of electron transport in Photosystem I with PsaC and PsaD proteins expressed in Escherichia coli. FEBS Lett 276: 175–180

    Article  PubMed  CAS  Google Scholar 

  • Zhao J, Li N, Warren P, Golbeck J and Bryant D (1992) Site-directed conversion of a cysteine to aspartate leads to the assembly of a [3Fe-4S] cluster in PsaC of Photosystem I –the photoreduction of FA is independent of FB. Biochemistry 31: 5093–5099

    Article  PubMed  CAS  Google Scholar 

  • Zhao J, Snyder WB, Mühlenhoff U, Rhiel E, Warren PV, Golbeck JH and Bryant DA (1993) Cloning and characterization of the psaE gene of the cyanobacterium Synechococcus sp. PCC 7002 –characterization of a psaE mutant and overproduction of the protein in Escherichia coli. Mol Microbiol 9: 183–194

    Article  PubMed  CAS  Google Scholar 

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Antonkine, M.L., Golbeck, J.H. (2006). Molecular Interactions of the Stromal Subunit PsaC with the PsaA/PsaB Heterodimer. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_8

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