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Genetic and Proteomic Studies of Sulfur Oxidation in Chlorobium tepidum (syn. Chlorobaculum tepidum)

  • Chapter
Sulfur Metabolism in Phototrophic Organisms

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 27))

The oxidation of reduced sulfur compounds is perhaps the most poorly understood physiological process carried out by the green sulfur bacteria (the Chlorobiaceae). My laboratory is testing models of sulfur oxidation pathways in the model system Chlorobium tepidum (ATCC 49652 syn. Chlorobaculum tepidum (Imhoff, 2003) ) by the creation and analysis of mutant strains lacking specific gene products. The availability of a complete, annotated genome sequence for C. tepidum enables this approach, which will specify targets for biochemical analysis by indicating which genes are important in an organismal context. This is particularly important when several potentially redundant enzymes are encoded by the genome for a particular reaction, such as sulfide oxidation. Additionally, we are using proteomics approaches to define the subcellular locations of proteins involved in sulfur oxidation pathways. The results produced by this research will refine models of anaerobic sulfur oxidation pathways and their integration into the global physiology of the Chlorobiaceae.

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Authors and Affiliations

  1. College of Marine and Earth Studies and Delaware Biotechnology Institute, University of Delaware, 19711, Newark, DE, USA

    Leong-Keat Chan, Rachael Morgan-Kiss & Thomas E. Hanson

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  1. Leong-Keat Chan
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  2. Rachael Morgan-Kiss
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  3. Thomas E. Hanson
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  1. University of Heidelberg, Im Neuenheimer Feld 360, 69120, Heidelberg, Germany

    Rüdiger Hell

  2. University of Bonn, Meckenheimer Allee 168, 53115, Bonn, Germany

    Christiane Dahl

  3. Texas Tech University, 79409-1061, Lubbock, TX, USA

    David Knaff

  4. Rutgers University, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA

    Thomas Leustek

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Chan, LK., Morgan-Kiss, R., Hanson, T.E. (2008). Genetic and Proteomic Studies of Sulfur Oxidation in Chlorobium tepidum (syn. Chlorobaculum tepidum). In: Hell, R., Dahl, C., Knaff, D., Leustek, T. (eds) Sulfur Metabolism in Phototrophic Organisms. Advances in Photosynthesis and Respiration, vol 27. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6863-8_18

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