Abstract
To evaluate overwintering strategies of the fungus Neozygites floridana, an important natural enemy of Tetranychus urticae, hibernating T. urticae females were investigated for the presence of fungal structures throughout one winter (October 12, 2006 to February 19, 2007) in field-grown strawberries in a cold climate in Norway (min. ambient temp −15.3°C). Neozygites floridana was present as hyphal bodies inside live, hibernating females in T. urticae populations throughout the sampling period. The lowest percentages of hibernating females with hyphal bodies were found at the two first dates of sampling at 5.5 and 0% on October 12 and 19, respectively. The prevalence then increased and peaked at 54.4% on January 14. Resting spores (immature) were also found in live hibernating females at some dates, but at lower prevalence than for hyphal bodies and predominantly only until November 8. Prevalence of resting spores in live hibernating females ranged from 2.5 to 13.8%. Total number of T. urticae was also recorded, and most mites of all four categories (nymphs, males, non-hibernating and hibernating females) were found at the first sampling date. At this date non-hibernating females were the most abundant. A sharp decrease in non-hibernating females, nymphs and males was, however, seen from mid-October to mid-November; also numbers of hibernating females decreased, but not as fast. The relative abundance of hibernating females compared to non-hibernating females increased from 32.2% at the first collection (October 12) to 97.7% at the last collection (February 2). This study confirms that N. floridana survives the winter as a semi-latent hyphal body infection, protected inside live hibernating females. It is therefore ready to develop and sporulate as soon as climatic conditions permit, resulting in early season infection of T. urticae.
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References
Brahde R (1970) Solas stilling i Norge Universitetsforlaget, Oslo, Norway (In Norwegian)
Brandenburg RL, Kennedy GG (1981) Overwintering of the pathogen Entomophthora floridana and its host, the twospotted spider mite. J Econ Entomol 74:428–431
Boykin LS, Campbell WV, Beute MK (1984) Effect of pesticides on Neozygites floridana (Entomophthorales: Entomophthoraceae) and arthropod predators attacking the twospotted spider mite (Acari: Tetranychidae) in North Carolina peanut fields. J Econ Entomol 77:969–975
Carner GR (1976) A description of the life cycle of Entomophthora sp. in the two-spotted spider mite. J Invertebr Pathol 28:245–254
Carner GR, Canerday TD (1970) Entomophthora sp. as a factor in the regulation of the two-spotted spider mite on cotton. Ecol Entomol 63:638–640
Cranham JE, Helle W (1985) Pesticide resistance in tetranychidae. In: Helle W, Sabelis MW (eds) Spider mites their biology, natural enemies and control, vol 1B. Elsevier, Amsterdam, pp 405–421
Cross JV, Easterbrook MA, Crook AM, Crook D, Fitzgerald JD, Innocenzi PJ, Jay CN, Solomon MG (2001) Review: natural enemies and biocontrol of pests of strawberry in northern and central Europe. Biocontrol Sci Technol 11:165–216
Delalibera I Jr, De Moraes GJ, Lapointe ST, Da Silva CAD, Tamai MA (2000) Temporal variability and progression of Neozygites sp. (Zygomycetes: Entomophthorales) in populations of Mononychellus tanajoa (Bondar) (Acari: Tetranychidae). Ann Soc Entomol Brasil 29:523–535
Delalibera I Jr, Demétrio CGB, Manly BFJ, Hajek AE (2006) Effect of relative humidity and of isolates of Neozygites tanajoae (Zygomycetes: Entomophthorales) on production of conidia from cassava green mite, Mononychellus tanajoae (Acari: Tetranychidae), cadavers. Biol Control 39:489–496
Devine GJ, Barber M, Denholm I (2001) Incidence and inheritance of resistance to METI-acaricides in European strains of the two-spotted spider mite (Tetranychus urticae) (Acari: Tetranychidae). Pest Manage Sci 57:443–448
Dick GL, Buschman LL (1995) Seasonal occurrence of a fungal pathogen, Neozygites adjarica (Entomophthorales: Neozygitaceae), infecting banks grass mites, Oligonychus pratensis and twospotted spider mites, Tetranychus urticae (Acari: Tetranychidae), in field corn. J Kans Entomol Soc 64:425–436
Easterbrook MA, Fitzgerald JD, Solomon MG (2001) Biological control of strawberry tarsonemid mite Phytonemus pallidus and two-spotted spider mite Tetranychus urticae on strawberry in the UK using species of Neoseiulus (Amblyseius) (Acari: Phytoseiidae). Exp Appl Acarol 25:25–36
Elliot SL (1998) Ecology and epizootiology of Neozygites floridana, a pathogen of the cassava green mite. Imperial College at Silwood Park, Berkshire, United Kingdom. (Ph.D. Thesis)
Elliot SL, Mumford JD, de Moraes GJ (2002) The role of resting spores in the survival of the mite-pathogenic fungus Neozygites floridana from Mononychellus tanajoa during dry periods in Brazil. J Invertebr Pathol 81:148–157
Garcia-Mari F, Gonzalez-Zamora JE (1999) Biological control of Tetranychus urticae (Acari: Tetranychidae) with naturally occurring predators in strawberry plantings in Valencia, Spain. Exp Appl Acarol 23:487–495
Greco NM, Pereyra PC, Guillade A (2005) Host-plant acceptance and performance of Tetranychus urticae (Acari: Tetranychidae). J Appl Entomol 130:32–36
Hajek AE (1997) Ecology of terrestrial fungal entomopathogens. In: Jones JG (ed) Advances in microbial ecology. Plenum Press, New York, pp 193–247
Hajek AE, Humber RA (1997) Formation and germination of Entomophaga maimaiga azygospores. Can J Bot 75:1739–1747
Helle W (1962) Genetics of resistance to organophosphorus compounds and its relation to diapause in Tetranychus urticae (Koch (Avari: Tetranychidae). T Pl Ziekten 68:155–195
Humber RA (1997) Fungi: Identification. In: Lacey LA (ed) Manual of techniques in insect pathology. Academic Press, San Diego, pp 153–185
Hutchins SH (1994) Techniques for sampling arthropods in integrated pest management. In: Pedigo LP, Buntin GD (eds) Handbook of sampling methods for arthropods in agriculture. CRC Press, London, pp 74–96
Keller S (1991) Arthropod-pathogenic entomophthorales of Switzerland. II. Erynia, Erynopsis, Neozygites, Zoophthora and Tarichum. Sydowia 43:39–122
Klingen I, Westrum K (2007) The effect of pesticides used in strawberries on the phytophagous mite Tetranychus urticae (Acari: Tetranychidae) and its fungal natural enemy Neozygites floridana (Zygomycetes: Entomophthorales). Biol Control 43:222–230
Klubertanz TH, Pedigo LP, Carlson RE (1991) Impact of fungal epizootics on the biology and management of the two spotted spider mite (Acari: Tetranychidae) in soybean. Environ Entomol 20:731–735
Mietkiewski R, Balazy S, van der Geest LPS (1993) Observations on a mycosis of spider mites (Acari: Teranychidae) caused by Neozygites floridana in Poland. J Invertebr Pathol 61:317–319
MINITAB (2006) Relase 14. State College, USA
Nemoto H, Aoki J (1975) Entomophthora floridana (Entomophthorales: Entomophthoraceae) attacking the sugi spider mite, Oligonychus hondoensis (Acarina : Tetranychidae), in Japan. Appl Ent Zool 10:90–95
Nilsen C, Steenberg T (2004) Entomophthoralean fungi infecting the bird cherry-oat aphid, Rhopalosiphum padi, feeding on its winter host bird cherry, Prunus padus. J Invertebr Pathol 87:70–73
Nordengen I, Klingen I (2006) Comparison of methods for estimating the prevalence of Neozygites floridana in Tetranychus urticae populations infesting strawberries. J Invertebr Pathol 92:1–6
Oduor GI, Yaninek JS, DeMoraes GJ, van der Geest LPS (1997) The effect of pathogen dosage on the pathogenicity of Neozygites floridana (Zygomycetes: Entomophthorales) to Mononychellus tanajoa (Acari: Tetranychidae). J Invertebr Pathol 70:127–130
Raworth DA (1986) An economic threshold function for the twospotted spider mite, Tetranychus urticae (Acari: Tetranychidae), on strawberries. Canadian Entomol 118:9–6
Sances FV, Wyman JA, Ting IP (1979) Physiological responses to spider mite infestation on strawberries. Environ Entomol 8:711–714
Sances FV, Wyman JA, Ting IP, Vansteenwyk RA, Oatman ER (1981) Spider mite interactions with photosynthesis, transpiration and productivity of strawberry (Acari: Tetranychidae). Environ Entomol 10:442–448
Sances FV, Toscano NC, Oatman ER, Lapre LF, Johnson MW, Voth V (1982) Reductions in plant processes by Tetranychus urticae (Acari: Tetranychidae) feeding on strawberry. Environ Entomol 11:733–737
Smitley DR, Brooks WM, Kennedy GG (1986a) Environmental effects on production of primary and secondary conidia, infection, and pathogenesis of Neozygites floridana, a pathogen of the twospotted spider mite, Tetranychus urticae. J Invertebr Pathol 47:325–332
Smitley DR, Kennedy GG, Brooks WM (1986b) Role of the entomogenous fungus, Neozygites floridana, in population declines of the twospotted spider mite, Tetranychus urticae in field corn. Entomol Exp Appl 41:255–264
Steinkraus DC (2000) Documentation of naturally-occurring pathogens and their impact in agroecosystems. In: Lacey L, Kaya Field HK (eds) Manual of techniques in invertebrate pathology. Kluwer Academic Publishers, The Netherlands, pp 303–320
Stenseth C (1965) Cold hardiness in the two-spotted spider mite (Tetranychus urticae Koch). Entomol Exp Appl 8:33–38
Stenseth C (1976) Overvintring av veksthusspinnmidd (Tetranychus urticae) i jordbærfelt. Gartneryrket 66:374–376 (In Norwegian)
Thomsen L, Brescani J, Eilenberg J (2001) Formation and germination of resting spores from different strains from the Entomophthora muscae complex produced in Musca domestica. Can J Bot 79:1076–1082
van der Geest LPS, Elliot SL, Breeuwer JAJ, Beerling EAM (2000) Diseases of mites. Exp Appl Acarol 24:497–560
Tillotson DK, Margolies DC (1990) Effect of cadaver age on production of infective stages of Entomophaga gryllii pathotype 2 in infected Melanoplus differentialis. J Invertebr Pathol 55:202–206
Veerman A (1977a) Aspects of the induction of diapause in a laboratory strain of the mite Tetranychus urticae. J Insect Physiol 23:703–711
Veerman A (1977b) Photoperiodic termination of diapause in spider mites. Nature 266:526–527
Veerman A (1985) Diapause. In: Helle W, Sabelis MW (eds) Spider mites their biology, natural enemies and control, vol 1A. Elsevier, Amsterdam, pp 279–316
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Klingen, I., Wærsted, G., Westrum, K. (2008). Overwintering and prevalence of Neozygites floridana (Zygomycetes: Neozygitaceae) in hibernating females of Tetranychus urticae (Acari: Tetranychidae) under cold climatic conditions in strawberries. In: Bruin, J., van der Geest, L.P.S. (eds) Diseases of Mites and Ticks. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9695-2_19
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