Abstract
Purified protein preparations, which recently were used only in biochemical laboratories, nowadays are coming more and more into our everyday life as medicine preparations and detergents. They are used in fine organic synthesis and food production as well as in a variety of analytical techniques. One of the effective methods of protein separation and purification is protein precipitation (liquid phase splitting) with the help of polyelectrolytes. The most extensively studied protein complexes are mixtures of lysozyme1, albumins2-4, gelatin5,6, and catalase7,8 with weak and strong polyelectrolytes of linear and crosslinked structure.
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References
J. Y. Shieh and C. E. Glatz, Precipitation of proteins with polyelectrolytes: Role of polymer molecular weight, In: Macromolecular Complexes in Chemistry and Biology, edited by P. Dubin, J. Block, R. Davis, D. N. Schulz, and C. Thies, (Springer-Verlag, Berlin, 1995). pp. 273–284.
E. Kokufuta, Complexation of proteins with polyelectrolytes in a salt-free system and biochemical characteristics of the resulting complexes, In: Macromolecular Complexes in Chemistry and Biology, edited by P. Dubin, J. Block, R. Davis, D. N. Schulz, and C. Thies, (Springer-Verlag, Berlin, 1995). pp. 300–325.
K. W. Mattison, I. J. Brittain, and P. L. Dubin, Protein-polyelectrolyte phase boundary, Biotechnol. Prog. 11, 632–637 (1995).
A. Tsuboi, T. Izumi, M. Hirata, J. Xia, P. Dubin, and E. Kokufuta, Complexation of proteins with a strong polyanion in an aqueous salt-free system, Langmuir 12, 6295–6303 (1996).
J. Greener, B. A. Contestable, and M. D. Bale, Interaction of anionic surfactants with gelatin: Viscosity effects, Macromolecules 20, 2490–2498 (1987).
. W. A. Bowman, M. Rubinstein, and J. S. Tan, Polyelectrolyte-gelatin complexation: Light-scattering study, Macromolecules 30, 3262–3270 (1997).
G. A. Bektenova, E. A. Bekturov, and S.E. Kudaibergenov, Interaction of catalase with cationic hydrogels: Influence of pH, kinetics of process and isotherms of adsorption, Polym. Adv. Technol. 10, 141–145 (1999).
G. A. Bektenova, E. A. Bekturov, G. K. Sulekeshova, and S. E. Kudaibergenov, Interaction of amphoteric hydrogels with catalase: Influence of pH and ionic strength, Polym. Prepr. 41(1), 750–751 (2000).
J. Xia and P. L. Dubin, Protein-polyelectrolyte complexes, In: Macromolecular Complexes in Chemistry and Biology, edited by P. Dubin, J. Block, R. Davis, D. N. Schulz, and C. Thies, (Springer-Verlag, Berlin, 1995). pp. 247–271.
C. S. Patrickios, C. J. Jang, W. R. Hertler, and T. A. Hatton, Protein interactions with acrylic polyampholytes, Polym. Prepr. 34(1), 954–955 (1993).
C. S. Patrickios, C. J. Jang, W. R. Hertler, and T. A. Hatton, Protein interactions with acrylic polyampholytes, in: Macro-ion Characterization from Dilute Solutions to Complex Fluids, edited by K. S. Schmitz, (ACS Symposium Series, Washington DC, v. 548, Ch.19, p. 257–267 1994).
C. S. Patrickios, L. R. Sharma, S. P. Armes, and N. C. Billingham, Precipitation of a water-soluble ABC triblock methacrylic polyampholyte. Effects of time, polymer concentration, salt type and concentration, and presence of a protein, Langmuir 15, 1613–1620 (1999).
S. Nath, C. S. Patrickios, and T. A. Hatton, A turbidimetric titration study of the interaction of proteins with block and random acrylic polyampholytes, Biotechnol.Prog. 11(1), 99–103 (1995).
S. Nath, Complexation behavior of proteins with polyelectrolytes and random acrylic polyampholytes using turbidimetric titration, J. Chem. Tech. Biotech. 62(3), 295–300 (1995).
C. S. Patrickios, W. R. Hertler, and T. A. Hatton, Protein complexation with acrylic polyampholytes, Biotechnol. Bioeng. 44, 1031–1039 (1994).
S. E. Kudaibergenov and E. A. Bekturov, Influence of the coil-globule confromational transition in polyampholytes on sorption and desorption of polyelectrolytes and human serum albumin, Vysokomol. Soedin. Ser.A. 31, 2614–2617 (1989).
A. K. Tultaev, S. E. Kudaibergenov, and E. A. Bekturov, Study of complexation of synthetic polyampholyte with human serum albumin, Izv. Akad. Nauk KazSSR, Ser. Khim. 6, 67–71 (1990).
H. Morawetz and W. L. Hughes, The interaction of proteins with synthetic polyelectrolytes. 1. Complexing of bovine serum albumin, J. Phys. Chem. 56, 64–69 (1952).
L. S. Rodkey and A. Hirata, Studies of ampholyte-protein interactions, Prot. Biol. Fluides, 34, 745–748 (1986).
O. Sh. Kurmanaliev, E. M. Shaikhutdinov, Sh. S. Tulbaev, and T. M. Mukhametkaliev, Influence of water on radical copolymerization of 1,2,5-trimethyl-4-vinylethynylpiperidinol-4 with methacrylic acid, Vysokomolek. Soedin. Ser B, 22, 526–528 (1980).
S. E. Kudaibergenov, Synthesis and characterization of Schiff base polyampholytes (to be published).
R. C. Schulz, M. Schmidt, E. Schwarzenbach, J. Zoller, Some new polyelectrolytes, Macromol.Chem., Macromol. Symp. 26, 221–231 (1989).
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Didukh, A.G., Makysh, G.S., Bimendina, L.A., Kudaibergenov, S.E. (2003). Bovine Serum Albumine Complexation with Some Polyampholytes. In: Geckeler, K.E. (eds) Advanced Macromolecular and Supramolecular Materials and Processes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8495-1_20
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DOI: https://doi.org/10.1007/978-1-4419-8495-1_20
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