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The T Cell Marker RT6 in a Rat Model of Autoimmune Diabetes

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ADP-Ribosylation in Animal Tissues

Abstract

The RT6 alloantigenic system of the rat was discovered in the 1970s. It was originally designated Pta, AgF, A.R.T.-2, and RTLy-2 by the laboratories involved in its characterization. Exchange of reagents demonstrated that these laboratories had identified the same system, and the official designation RT6 was assigned in 1982 (1).

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References

  1. Lubaroff, D. M., G. Butcher, C. DeWitt, T. J. Gill, III, E. Günther, J. Howard, and K. Wonigeit. 1983. Fourth international workshop on alloantigenic systems in the rat. Transplant. Proc. 15:1683

    Google Scholar 

  2. Koch, F., A. Kashan, and H.-G. Thiele. 1988. The rat T-cell differentiation marker RT6.1 is more polymorphic than its alloantigenic counterpart RT6.2. Immunology 65:259–265.

    PubMed  CAS  Google Scholar 

  3. Koch, F., H. G. Thiele, and M. G. Low. 1986. Release of the rat T cell alloantigen RT-6.2 from cell membranes by phosphatidylinositol-specific phospholipase C. J. Exp. Med. 164:1338–1343.

    Article  PubMed  CAS  Google Scholar 

  4. Thiele, H.-G., F. Koch, and A. Kashan. 1987. Postnatal distribution profiles of Thy-1+ and RT6+ cells in peripheral lymph nodes of DA rats. Transplant. Proc. 19:3157–3160.

    Google Scholar 

  5. Mojcik, C.R, D. L. Greiner, E. S. Medlock, K. L. Komschlies, and I. Goldschneider. 1988. Characterization of RT6 bearing rat lymphocytes. I. Ontogeny of the RT6+ subset. Cell. Immunol. 114:336–346.

    Article  PubMed  CAS  Google Scholar 

  6. Mojcik, C. R, D. L. Greiner, and I. Goldschneider. 1991. Characterization of RT6-bearing lymphocytes. II. Developmental relationships of RT6 and RT6+ T cells. Develop. Immunol. 1:191–201.

    Article  CAS  Google Scholar 

  7. Fangmann, J., R. Schwinzer, and K. Wonigeit. 1991. Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: Predominance of T cell receptor α/β+/CD2 cells and high expression of the RT6 alloantigen. Eur. J. Immunol. 21:753–760.

    Article  PubMed  CAS  Google Scholar 

  8. Waite, D. J., M. C. Appel, E. S. Handler, J. P. Mordes, A. A. Rossini, and D. L. Greiner. 1996. Ontogeny and immunohistochemical localization of thymus dependent and thymus independent RT6+ cells in the rat. Am. J. Pathol. (In Press)

    Google Scholar 

  9. Waite, D. J., E. S. Handler, J. P. Mordes, A. A. Rossini, and D. L. Greiner. 1993. The RT6 rat lymphocyte alloantigen circulates in soluble form. Cell. Immunol. 152:82–95.

    Article  PubMed  CAS  Google Scholar 

  10. Koch, R, R Haag, A. Kashan, and H.-G. Thiele. 1990. Primary structure of rat RT6.2, a nonglycosylated phosphatidylinositol-linked surface marker of postthymic T cells. Proc. Natl. Acad. Sci. USA 87:964–967.

    Article  PubMed  CAS  Google Scholar 

  11. Haag, R, R Koch, and H.-G. Thiele. 1990. Nucleotide and deduced amino acid sequence of the rat T-cell alloantigen RT6.1. Nucleic Acids Res. 18:1047

    Article  PubMed  CAS  Google Scholar 

  12. Hosseinzadeh, H. and I. Goldschneider. 1993. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J. Immunol. 150:1670–1679.

    Google Scholar 

  13. Groen, H., R A. Klatter, N. H. C. Brons, A. S. Wubbena, P. Nieuwenhuis, and J. Kampinga. 1995. High-frequency, but reduced absolute numbers of recent thymic migrants among peripheral blood T lymphocytes in diabetes-prone BB rats. Cell. Immunol. 163:113–119.

    Article  PubMed  CAS  Google Scholar 

  14. Greiner, D. L., J. P. Mordes, M. Angelillo, E. S. Handler, C. R Mojcik, N. Nakamura, and A. A. Rossini. 1988. Role of regulatory RT6+ T-cells in the pathogenesis of diabetes mellitus in BB/Wor rats. In Frontiers in diabetes research: Lessons from animal diabetes II. E. Shafrir and A. E. Renold, editors. John Libbey, London.58–67.

    Google Scholar 

  15. Hunt, H. D. and D. M. Lubaroff. 1992. Identification of functional T cell subsets and surface antigen changes during activation as they relate to RT6. Cell. Immunol. 143:194–211.

    Article  PubMed  CAS  Google Scholar 

  16. Greiner, D. L., J. P. Mordes, E. S. Handler, M. Angelillo, N. Nakamura, and A. A. Rossini. 1987. Depletion of RT6.1+ T lymphocytes induces diabetes in resistant BioBreeding/Worcester (BB/W) rats. J. Exp. Med. 166:461–475.

    Article  PubMed  CAS  Google Scholar 

  17. Fangmann, J., R. Schwinzer, H.-J. Hedrich, I. Klöting, and K. Wonigeit. 1991. Diabetes-prone BB rats express the RT6 alloantigen on intestinal intraepithelial lymphocytes. Eur. J. Immunol. 21:2011–2015.

    Article  PubMed  CAS  Google Scholar 

  18. Fangmann, J., R. Schwinzer, H.-J. Hedrich, and K. Wonigeit. 1993. Demonstration of RT6 expression on a CD3 population of intestinal intraepithelial lymphocytes of athymic nude rats. Transplant. Proc. 25:2789–2790.

    PubMed  CAS  Google Scholar 

  19. Rossini, A. A., J. P. Mordes, E. S. Handler, and D. L. Greiner. 1995. Human autoimmune diabetes mellitus: Lessons from BB rats and NOD mice— Caveat emptor. Clin. Immunol. Immunopathol. 74:2–9.

    Article  CAS  Google Scholar 

  20. Mordes, J., R. Bortell, J. Doukas, M. R. Rigby, B. J. Whalen, D. Zipris, D. L. Greiner, and A. A. Rossini. 1996. The BB/Wor rat and the balance hypothesis of autoimmunity. Diabetes/Metab. Rev. (In Press)

    Google Scholar 

  21. Crisá, L., J. P. Mordes, and A. A. Rossini. 1992. Autoimmune diabetes mellitus in the BB rat. Diabetes/Metab. Rev. 8:9–37.

    Article  Google Scholar 

  22. Guberski, D. L. 1994. Diabetes-prone and diabetes-resistant BB rats: Animal models of spontaneous and virally induced diabetes mellitus, lymphocytic thyroiditis, and collagen-induced arthritis. ILAR News 35:29–37.

    Google Scholar 

  23. Greiner, D. L., E. S. Handler, K. Nakano, J. P. Mordes, and A. A. Rossini. 1986. Absence of the RT-6 T cell subset in diabetes-prone BB/W rats. J. Immunol. 136:148–151.

    PubMed  CAS  Google Scholar 

  24. McKeever, U., J. P. Mordes, D. L. Greiner, M. C. Appel, J. Rozing, E. S. Handler, and A. A. Rossini. 1990. Adoptive transfer of autoimmune diabetes and thyroiditis to athymic rats. Proc. Natl. Acad. Sci. USA 87:7718–7722.

    Article  Google Scholar 

  25. Joseph, S., A. G. Diamond, W. Smith, J. D. Baird, and G. W. Butcher. 1993. BB-DR/Edinburgh: A lymphopenic, non-diabetic subline of BB rats. Immunology 78:318–328.

    PubMed  CAS  Google Scholar 

  26. Whalen, B. J., D. L. Greiner, J. P. Mordes, and A. A. Rossini. 1994. Adoptive transfer of autoimmune diabetes mellitus to athymic rats: Synergy of CD4+ and CD8+ T cells and prevention by RT6+ T cells. J. Autoimmun. 7:819–831.

    Article  PubMed  CAS  Google Scholar 

  27. Ellerman, K. and A. Like. 1995.Regulatory T cells in rat autoimmune diabetes are strain dependent. Diabetes 44 (Suppl. l):138A(Abstr.)

    Google Scholar 

  28. Fowell, D. and D. Mason. 1993. Evidence that the T cell repertoire of normal rats contains cells with the potential to cause diabetes. Characterization of the CD4+ T cell subset that inhibits this autoimmune potential. J. Exp. Med. 177:621–636.

    Article  Google Scholar 

  29. Bell, E. B. 1992. Function of CD4 T cell subsets in vivo: expression of CD45R isoforms. [Review]. Seminars in Immunology 4:43–50.

    PubMed  CAS  Google Scholar 

  30. Mordes, J. P., D. L. Gallina, E. S. Handler, D. L. Greiner, N. Nakamura, A. Pelletier, and A. A. Rossini. 1987. Transfusions enriched for W3/25+ helper/inducer T lymphocytes prevent spontaneous diabetes in the BB/W rat. Diabetologia 30:22–26.

    Article  PubMed  CAS  Google Scholar 

  31. Zipris, D., D. L. Greiner, S. Malkani, B. J. Whalen, J. P. Mordes, and A. A. Rossini. 1996. Cytokine gene expression in islets and thyroids of BB rats: Interferon gamma and IL-12 p40 mRNA increase with age in both diabetic and insulin treated nondiabetic BB rats. J. Immunol. 156:1315–1321.

    PubMed  CAS  Google Scholar 

  32. Takada, T., K. Iida, and J. Moss. 1994. Expression of NAD glycohydrolase activity by rat mammary adenocarcinoma cells transformed with rat T cell alloantigen RT6.2. J. Biol. Chem. 269:9420–9423.

    PubMed  CAS  Google Scholar 

  33. Haag, F., V. Andresen, S. Karsten, F. Koch-Nolte, and H.-G. Thiele. 1995. Both allelic forms of the rat T cell differentiation marker RT6 display nicotinamide adenine dinucleotide (NAD)-glycohydrolase activity, yet only RT6.2 is capable of automodification upon incubation with NAD. Eur: J. Immunol. 25:2355–2361.

    Article  CAS  Google Scholar 

  34. Rigby, M. R., R. Bortell, L. A. Stevens, J. Moss, T. Kanaitsuka, H. Shigeta, J. P. Mordes, D. L. Greiner, and A. A. Rossini. 1996. Rat RT6.2 and mouse Rt6 locus 1 are NAD+:arginine ADP-ribosyltransferases with auto-ADP-ribosylation activity. J. Immunol. (In Press)

    Google Scholar 

  35. Okazaki, I. J. and J. Moss. 1994. Common structure of the catalytic sites of mammalian and bacterial toxin ADP-ribosyltransferases.Mol. Cell Biochem. 138:177–181.

    Article  PubMed  CAS  Google Scholar 

  36. Rashba, E. J., E.-P. Reich, C. A. Janeway, and R. S. Sherwin. 1993. Type 1 diabetes mellitus: an imbalance between effector and regulatory T cells? Acta Diabetol. 30:61–69.

    Article  PubMed  CAS  Google Scholar 

  37. Koch, F., F. Haag, and H.-G. Thiele. 1990. Nucleotide and deduced amino acid sequence for the mouse homologue of the rat T-cell differentiation marker RT6. Nucleic Acids Res. 18:3636

    Article  PubMed  CAS  Google Scholar 

  38. Prochazka, M., H. R. Gaskins, E. H. Leiter, F. Koch-Nolte, F. Haag, and H.-G. Thiele. 1991. Chromosomal localization, DNA polymorphism, and expression of Rt-6, the mouse homologue of rat T-lymphocyte differentiation marker RT6. Immunogenetics 33:152–156.

    Article  PubMed  CAS  Google Scholar 

  39. Koch-Nolte, F., J. Klein, C. Hollmann, M. Kühl, F. Haag, H. R. Gaskins, E. Leiter, and H.-G. Thiele. 1995. Defects in the structure and expression of the genes for the T cell marker Rt6 in NZW and (NZB x NZW)F, mice. Int. Immunol. 7:883–890.

    Article  PubMed  CAS  Google Scholar 

  40. Koch-Nolte, F., D. Petersen, S. Balasubramanian, F. Haag, D. Kahlke, T. Wilier, R. Kastelein, F. Bazan, and H. G. Thiele. 1996. Mouse T cell membrane proteins Rt6-1 and Rt6-2 are arginine protein mono(AD-Pribosyl) transferases and share secondary structure motifs with ADP-ribosylating bacterial toxins. J. Biol. Chem. 271:7686–7693.

    Article  PubMed  CAS  Google Scholar 

  41. Haag, F., F. Koch-Nolte, M. Kühl, S. Lorenzen, and H.-G. Thiele. 1994. Premature stop codons inactivate the RT6 genes of the human and chimpanzee species. J. Mol. Biol. 243:537–546.

    Article  PubMed  CAS  Google Scholar 

  42. Lévy, I., Y. Q. Wu, N. Roeckel, F. Bulle, A. Pawlak, S. Siegrist, M. G. Mattéi, and G. Guellaen. 1996. Human testis specifically expresses a homologue of the rodent T lymphocytes RT6 mRNA. FEBS Lett. 382:276–280.

    Article  PubMed  Google Scholar 

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Greiner, D.L. et al. (1997). The T Cell Marker RT6 in a Rat Model of Autoimmune Diabetes. In: Haag, F., Koch-Nolte, F. (eds) ADP-Ribosylation in Animal Tissues. Advances in Experimental Medicine and Biology, vol 419. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8632-0_26

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  • DOI: https://doi.org/10.1007/978-1-4419-8632-0_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4652-4

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