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Optimizing Ammonia Pressurization/Depressurization Processing Conditions to Enhance Enzymatic Susceptibility of Dwarf Elephant Grass

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Twenty-First Symposium on Biotechnology for Fuels and Chemicals

Abstract

An ammonia pressurization/depressurization process was investigated to evaluate the potential of producing reducing sugars from dwarf elephant grass, a warm-season forage. Moisture, temperature, and ammonia loading affected sugar yield (p <; 0.0001). At optimal conditions, ammonia processing solubilized 50.9% of the hemicellulose and raised the sugar yield (percentage of theoretical) from 18 to 83%. Glucose and xylose production were increased 3.2- and 8.2-fold, respectively. The mild processing conditions of the ammonia treatment (90–100°C, 5 min), the low enzyme loading (2 international filter paper units/g), and the short hydrolysis time (24 h), greatly enhance the potential of using forages to produce sugars valuable for several applications.

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Ferrer, A., Byers, F.M., Sulbarán-de-Ferrer, B., Dale, B.E., Aiello, C. (2000). Optimizing Ammonia Pressurization/Depressurization Processing Conditions to Enhance Enzymatic Susceptibility of Dwarf Elephant Grass. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_12

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  • DOI: https://doi.org/10.1007/978-1-4612-1392-5_12

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7128-4

  • Online ISBN: 978-1-4612-1392-5

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