Abstract
Neurospora crassa X1 was found to ferment xylose and glucose simultaneously. Xylose was the appropriate inducer for the production of xylose reductase that had two isoenzymes designated as El and EIL Both El and EII, which were purified by affinity chromatography, had NADPH-dependent xylose reductase activities. EII also had NADH-dependent activity, and El is the only xylose reductase found so far without any NADH-dependent activity. El and EII had MWs of 30 kDa and 27 kDa, and pIs of 5.6 and 5.2, respectively. The specifities of El and EII against triose, pentoses, and hexoses were studied. The Kms against xylose for El and EII were 2.3 mM and 1.1 mM respectively, which were much lower than those of the xylose reductase from yeast.
Author to whom all correspondence and reprint requests should be addressed. Current address is Agricultural and Biological Engineering, Purdue University, W. Lafayette, IN 47907
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Zhao, X., Gao, P., Wang, Z. (1998). The Production and Properties of a New Xylose Reductase from Fungus Neurospora crassa. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_38
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DOI: https://doi.org/10.1007/978-1-4612-1814-2_38
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