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Downstream Processing of Inulinase Comparison of Different Techniques

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Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

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Abstract

Candida kefyr DSM 70106 was cultivated in a medium containing inulin as a carbon source. About 92% of the inulinase was recovered directly from the medium. Different concentration (Cf) and enrichment (Ef) factors were obtained, using the following methods: Cross-flow filtration (microfiltration and cell diafiltration were carried out using a rotary filter; enzyme ultrafiltration and diafiltration were performed using a cassette module): Cf = 7.5 and Ef = 2.2; liquid-liquid extraction of N-Benzyl-N-Dodecyl-N-bis[2-hydroxyethyl] ammonium chloride (BDBAC) reversed micelles: Cf = 2.5 and Ef = 2.7; and expanded-bed adsorption: Cf = 2.8 and Ef = 4.3.

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References

  1. Kierstan, M. (1980), Process Biochem. 15, 2–4.

    CAS  Google Scholar 

  2. Kaur, N., Kaur, M., Gupta, A. K., and Singh, R. (1992), J. Chem. Technol. Biotechnol. 53, 279–284.

    Article  CAS  Google Scholar 

  3. Hauly, M. C. O., Bracht, A., Beck, R., and Fontana, J. D. (1992), AppL Biochem. Biotechnol. 34/35, 297–308.

    Article  Google Scholar 

  4. Manzoni, M., and Cavazzoni, V. (1992), J. Chem. Technol. Biotechnol. 54, 311–315.

    Article  CAS  Google Scholar 

  5. Claessens, G., Van Laere, A., and De Proft, M. (1990), J. Plant Physiol. 136, 35–39.

    Article  CAS  Google Scholar 

  6. Manzoni, M., and Cavazzoni, V. (1988), Lebensm. Wiss. Technol. 21, 271–274.

    CAS  Google Scholar 

  7. Pessoa Jr., A; Vitolo, M., and Hustedt, H. (1996c) Appl. Biotechnol. Biochem. 57/58, 699–709.

    Article  CAS  Google Scholar 

  8. Parekh, S., and Margaritis, A. (1986), Agric. Biol. Chem. 50, 1085–1087.

    Article  CAS  Google Scholar 

  9. Krei, G., Meyer, U., Börner, B., and Hustedt, H. (1994), Bioseparation 5, 175–183.

    Google Scholar 

  10. Luisi, P. L., Giomini, M., Pileni, M. P., and Robinson, B. H. (1988), Biochim. Biophys. Acta 947, 209–246.

    Article  CAS  Google Scholar 

  11. Krei, G. A. and Hustedt, H. (1989), BioEngineering 3/4, p.32–41.

    Google Scholar 

  12. Frej, A.-K., B., Hjorth, R., and Hammarström, A. (1994), Biotechnol. Bioeng. 44, 922–929.

    Article  CAS  Google Scholar 

  13. Bradford, M. A. (1976), Anal Biochem. 72, 248–254.

    Article  CAS  Google Scholar 

  14. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951), J. Biol. Chem. 193, 265–275.

    CAS  Google Scholar 

  15. Kroner, K. H. and Schütte, H. (1989), in International Technical Conference on Membrane Separation Processes pp. 279–290.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Departamento de Biotecnologia, FAENQUIL-CP116, CEP. 12, 600-000, Lorena, SP, Brazil

    Adalberto Pessoa Jr.

  2. Biochemical and Pharmaceutical Technology Departmento, FCF/USP, P.O. Box 66083, CEP 05315-970, São Paulo, SP, Brazil

    Michele Vitolo

Authors
  1. Adalberto Pessoa Jr.
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  2. Michele Vitolo
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Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison

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© 1998 Springer Science+Business Media New York

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Pessoa, A., Vitolo, M. (1998). Downstream Processing of Inulinase Comparison of Different Techniques. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_46

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  • DOI: https://doi.org/10.1007/978-1-4612-1814-2_46

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7295-3

  • Online ISBN: 978-1-4612-1814-2

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