Skip to main content
SpringerLink
Log in

Ultrasensitive Enzyme Immunoassays for Inhibins and Activins

  • Chapter
Inhibin, Activin and Follistatin

Part of the book series: Serono Symposia USA ((SERONOSYMP))

Abstract

The “Monash” radioimmunoassay for inhibin (1) provided a tool for numerous physiological studies (2). However, it gradually became apparent that this assay was unable to discriminate between dimeric bioactive inhibin forms and various forms of the free alpha subunit that occur in large amounts in body fluids (3). It became popular in publications to refer to the material measurable by the Monash assay as “immunoreactive” inhibin to acknowledge the possibility that the assay might not accurately measure the levels of bioactive inhibin.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Robertson DM, Tsonis CG, McLachlan RI. Comparison of inhibin immunological and biological activity in human serum. J Clin Endocrinol Metab 1989;67:438–43.

    Article  Google Scholar 

  2. Burger HG. Clinical reviews. 46. Clinical utility of inhibin measurements. J Clin Endocrinol Metab 1993;76:1391–6.

    Article  PubMed  CAS  Google Scholar 

  3. Schneyer AL, Mason AJ, Burton LE, Ziegner JR, Crowley WF. Immunoreactive inhibin alpha subunit in human serum: implications for radioimmunoassay. J Clin Endocrinol Metab 1990;70:1208–12.

    Article  PubMed  CAS  Google Scholar 

  4. Baly D, Allison DE, Krummen LA, Woodruff T, Soules MR, Chen SA, et al. Development of a specific and sensitive two-site ELIS A for measurement of inhibin A concentrations in serum. Endocrinology 1993;132:2099–108.

    Article  PubMed  CAS  Google Scholar 

  5. Poncelet E, Franchimont P. Two site enzyme immunoassay of inhibin. Front Endocrinol 1994;3:45–54.

    CAS  Google Scholar 

  6. Groome NP, Hancock J, Betteridge A, Lawrence M, Craven R. Monoclonal and polyclonal antibodies reactive with the 1-32 amino-terminal peptide of the alpha subunit of human 32 K inhibin. Hybridoma 1990;9:31–42.

    Article  PubMed  CAS  Google Scholar 

  7. Groome NP, Illingworth P, O’Brien M, Priddle J, McNeilly A. A novel ultrasensitive two-site ELISA for the detection of inhibin pro-alpha C-like immunoreactivity in human serum. J Clin Endocrinol Metab 1995;80:2926–32.

    Article  PubMed  CAS  Google Scholar 

  8. Groome N, Illingworth PJ, O’Brien M, Cooke I, Ganesan TS, Baird DT, McNeilly AS. Detection of dimeric inhibin throughout the human menstrual cycle. Clin Endocrinol 1994;40:717–23.

    Article  CAS  Google Scholar 

  9. Groome NP, Illingworth P, O’Brien M, Pai R, Mather J, Rodger FE, McNeilly A. Measurement of circulating dimeric inhibin-B in the human menstrual cycle using a new ultrasensitive ELISA. J Clin Endocrinol Metab 1996;81:1401–5.

    Article  PubMed  CAS  Google Scholar 

  10. Groome NP, Lawrence M. Preparation of monoclonal antibodies reactive with the beta A subunit of human ovarian inhibin. Hybridoma 1991;10:309–16.

    Article  PubMed  CAS  Google Scholar 

  11. Knight PG, Muttukrishna S, Groome N. Development and application of a two-site enzyme immunoassay for the determination of total activin-A in serum and follicular fluid. J Endocrinol 1996;148:267–79

    Article  PubMed  CAS  Google Scholar 

  12. Evans L, Muttukrishna S, Knight P, Groome N. Development, validation, and application of a two-site ELISA specific for “total” activin-AB. J Endocrinol 1996; (in press).

    Google Scholar 

  13. Knight PG, Muttukrishna S. Measurement of dimeric inhibin using a modified two-site immunoradiometric assay specific for oxidised (metO) inhibin. J Endocrinol 1994;141:417–25.

    Article  PubMed  CAS  Google Scholar 

  14. Lambert GM, Hall JE, Sluss PM, Taylor AE, Martin KA, Groome NP, et al. Relatively low levels of dimeric inhibin circulate in men and women with polycystic ovarian syndrome using a specific two-site ELISA. J Clin Endocrinol Metab 1994;79:45–50.

    Article  Google Scholar 

  15. Muttukrishna S, Fowler PA, Groome NP, Mitchell GG, Robertson WR, Knight PG. Serum concentrations of dimeric inhibin during the spontaneuous human menstrual cycle and after treatment with exogenous gonadotrophins. Hum Reprod (Oxf) 1994;9:1634–42.

    CAS  Google Scholar 

  16. Cooke I, O’Brien M, Charnock FM, Groome NP, Ganesan TS. Dimeric inhibin as a marker for granulosa cell tumour of the ovary. Br J Cancer 1995;71:1046–50.

    Article  PubMed  CAS  Google Scholar 

  17. Wallace EM, Swanson IA, Groome NP. Serum inhibin-A in first trimester prenatal screening for Down’s syndrome. Prenatal Diagn 1995;15:359–62.

    Article  CAS  Google Scholar 

  18. Wallace EM, Swanson IA, McNeilly A, Blundell G, Calder AA, Groome NP. Second trimester prenatal screening for Down’s syndrome using serum inhibin-A. Clin Endocrinol 1994;44:17–21.

    Article  Google Scholar 

  19. Muttukrishna S, George L, Fowler, PA, Groome NP, Knight P. Measurement of serum dimeric inhibin-A concentrations in human pregnancy. Clin Endocrinol 1994;42:391–7.

    Article  Google Scholar 

  20. Billiar R, Hemmings R, Smith P, Groome, NP. Identification of biologically active inhibin in the peritoneal fluid of women. J Assist Reprod Genet 1995;12:55–60.

    Article  PubMed  CAS  Google Scholar 

  21. Fahy PA, Wilson CA, Beard AJ, Groome NP, Knight P. Changes in inhibin-A and total inhibin in peripheral serum and ovaries of rats after gonadotropin-induced follicular development and during the normal oestrous cycle. JEndocrinol 1995;147:271–83.

    Article  CAS  Google Scholar 

  22. Price C, Carriere P, Bhatia B, Groome NP. Comparison of hormonal and histological changes during follicular growth as measured by ultrasonography in cattle. J Reprod Fertil 1995;103:63–8.

    Article  PubMed  CAS  Google Scholar 

  23. Price CA, Salah N, Groome NP. Plasma concentrations of dimeric inhibin and oestradiol in heifers undergoing superovulation with eCG or FSH. J Reprod Fertil 1995;103(suppl 49):507–10.

    Google Scholar 

  24. Robertson D, Burger HG, Sullivan J, Cahir N, Groome N, Poncelet E, et al. Biological and immunological characterization of inhibin forms in human plasma. Clin Endocrinol Metab 1996;81:669–76.

    Article  CAS  Google Scholar 

  25. Aitken DA, Wallace E, Crossley JA, Swanston IA, Pareren YV, Maarle MV, et al. Dimeric inhibin-A as a marker for Down’s syndrome and trisomy 18 pregnancies at 7–18 weeks gestation. N Eng J Med 1996;334:1231–6.

    Article  CAS  Google Scholar 

  26. Illingworth P, Groome NP, Duncan WC, Grant V, Tovanabutra S, Baird DT, et al. Circulating forms of inhibin in human pregnancy. J Clin Endocrinol Metab 1996;81:1471–5.

    Article  PubMed  CAS  Google Scholar 

  27. Smith LC, Olivera-Angel M, Groome NP, Bhatia B, Price CA. Oocyte quality in small antral follicles in the presence or absence of a large dominant follicle in cattle. J Reprod Fertil 1996;106:193–9.

    Article  PubMed  CAS  Google Scholar 

  28. Lockwood GM, Muttukrishna S, Groome NP, Knight PG, Ledger WL. Circulating inhibin and activin during GnRH downregulation and ovarian hyperstimulation with recombinant FSH for in vitro fertilization. Clin Endocrinol (in press).

    Google Scholar 

  29. Muttukrishna S, Child TJ, Groome NP, Ledger WL. Feto-placental unit is the major source of inhibin-A and activin-A in early pregnancy. J Endocrinol (in press).

    Google Scholar 

  30. Klein N, Illingworth P, Groome NP, McNeilly A, Battaglia DE, Soules M. Decreased inhibin-B secretion is associated with the monotropic FSH rise in older ovulatory women: a study of serum and follicular fluid inhibin-A and B levels in spontaneous menstrual cycles. J Clin Endocrinol Metab 1966;81:2742–5.

    Article  Google Scholar 

  31. Woodruff T, Besecke LM, Groome NP, Draper LB, Schwartz N, Weiss J. Inhibin-A and B are inversely correlated to FSH yet are discordant in the follicular phase and are expressed in a sexually dimorphic manner in the rat. Endocrinology (in press).

    Google Scholar 

  32. Lambert-Messerlian GM, Crowley WF, Schneyer AL. Extragonadal alpha inhibin precursor proteins circulate in male serum. J Clin Endocrinol Metab 1995;80:3043–9.

    Article  PubMed  CAS  Google Scholar 

  33. Illingworth P, Groome, NP, Byrd W, Rainey WE, McNeilly A, Mather JP, et al. Inhibin-B: a likely candidate for the physiologically important form of inhibin in men. J Clin Endocrinol Metab 1996;81:1321–5.

    Article  PubMed  CAS  Google Scholar 

  34. McCullagh GR. Dual endocrine control in the testis. Science 1932;76:19.

    Article  PubMed  CAS  Google Scholar 

  35. Anawalt BD, Bebb RA, Matsumot AM, Groome NP, Illingworth P, McNeilly A, et al. Serum inhibin-B levels reflect Sertoli cell function in normal men and in men with testicular failure. J Clin Endocrinol Metab 1996;81:3341–5.

    Article  PubMed  CAS  Google Scholar 

  36. Muttukrishna S, Fowler PA, George L, Groome NP, Knight PG. Changes in peripheral serum levels of “total” activin-A during the human menstrual cycle and pregnancy. J Clin Endocrinol Metab 1996;81:3328–34.

    Article  PubMed  CAS  Google Scholar 

  37. Rose MP, Gaines Das RE. International collaborative study by in vitro bioas-says and immunoassays of the first international standard for inhibin, human recombinant. 1996;24:1–18.

    CAS  Google Scholar 

  38. Bhasin S, De Kretser D. Editorial: Measurement of circulating inhibin levels: revising the inhibin hypothesis. J Clin Endocrinol Metab 1996;81:1318–20.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Nigel Groome
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Illingworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shanthi Muttukrishna
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Philip Knight
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lee Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Martin O’Brien
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Alan McNeilly
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Obstetrics and Gynecology School of Medicine, The University of Tokushima, 3-18-15, Kuramoto-cho, Tokushima, 770, Japan

    Toshihiro Aono M.D., Ph.D. (Professor and Chairman) (Professor and Chairman)

  2. Division of Enzyme Cytology Institute for Enzyme Research, The University of Tokushima, 3-18-15, Kuramoto-cho, Tokushima, 770, Japan

    Hiromu Sugino Ph.D.

  3. The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Wylie W. Vale Ph.D.

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media New York

About this chapter

Cite this chapter

Groome, N. et al. (1997). Ultrasensitive Enzyme Immunoassays for Inhibins and Activins. In: Aono, T., Sugino, H., Vale, W.W. (eds) Inhibin, Activin and Follistatin. Serono Symposia USA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-1874-6_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-1874-6_11

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7320-2

  • Online ISBN: 978-1-4612-1874-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation