Skip to main content
SpringerLink
Log in

The Anion Transport Protein

Structure and Function

  • Chapter
The Red Cell Membrane

Part of the book series: Contemporary Biomedicine ((CB,volume 10))

Abstract

The major 95 kdalton transmembrane protein of the red-cell membrane, denoted band 3 (Fairbanks et al., 1971) or capnophorin (Wieth and Bjerrum, 1983), catalyzes a tightly coupled exchange of anions. The purpose of this chapter is to summarize the current state of knowledge regarding the structure of this protein, with emphasis on those aspects of the structure that relate to anion transport. The amount of information currently available about band 3 is quite large, and this chapter will deal only superficially with several important topics that are somewhat related to the transport mechanism. These include lateral and rotational mobility of the protein (Beth et al., 1986; Mühlebach and Cherry, 1985); carbohydrate composition and structure (Tsuji et al., 1981; Fukuda et al., 1984); band 3 biosynthesis (Braell and Lodish, 1981); role of band 3 in red-cell senescence (Kay, 1984; Low et al., 1985); interaction with lipids (Köhne et al., 1983); and the physiology of CO2 exchange in the pulmonary and systemic capillaries (see Wieth et al., 1982). Certain other topics that relate centrally to the transport mechanism, including transport kinetics, reversible inhibitors, pH dependence, and chemical modification of arginine residues and carboxyl groups, are discussed in detail in Chapters 9 and 15.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alper, S., Kopito, R. R., and Lodish, H. F. (1987) A molecular biological approach to the study of anion transport. Kidney International 32, Suppl. 23 S117–S128.

    CAS  Google Scholar 

  • Appell, K. C. and Low, P. S. (1981) Partial structural characterization of the cytoplasmic domain of the erythrocyte membrane protein, band 3. J. Biol. Chem. 256 11104–11111.

    PubMed  CAS  Google Scholar 

  • Appell, K. E. and Low, P. S. (1982) Evaluation of structural independence of membrane spanning and cytoplasmic domains of band 3. Biochemistry 21 2151–2157.

    Article  PubMed  CAS  Google Scholar 

  • Barzilay, M. and Cabantchik, Z. I. (1979) Anion transport in red blood cells. III. Sites and sideness of inhibition by high affinity reversibly binding probes. Membr. Biochem. 2 297–322.

    Article  PubMed  CAS  Google Scholar 

  • Bennett, V. (1985) The membrane skeleton of human erythrocytes and its implications for more complex cells. Ann. Rev. Biochem. 54 273–304.

    Article  PubMed  CAS  Google Scholar 

  • Bennett, V. and Stenbuck, P. J. (1980) Association between ankyrin and the cytoplasmic domain of band 3 isolated from the human erythrocyte membrane. J. Biol. Chem. 255 6424–6432.

    PubMed  CAS  Google Scholar 

  • Benz, R., Tosteson, M. T., and Schubert, D. (1984) Formation and properties of tetramers of band 3 protein from human erythrocyte membranes in planar lipid bilayers. Biochim. Biophys. Acta 775 347–355.

    Article  PubMed  CAS  Google Scholar 

  • Berghout, A., Raida, M., Romano, L., and Passow, H. (1985) pH dependence of phosphate transport across the red blood cell membrane after modification by dansyl chloride. Biochim. Biophys. Acta 815 281–286.

    Article  PubMed  CAS  Google Scholar 

  • Beth, A. H., Conturo, T. E., Venkataramu, S. D., and Staros, J. D. (1986) Dynamics and interactions of the anion channel in intact human erythrocytes: An electron paramagnetic resonance spectroscopic study employing a new membrane-impermeant bifunctional spin-label. Biochemistry 25 3824–3832.

    Article  PubMed  CAS  Google Scholar 

  • Braell, W. A. and Lodish, H. F. (1981) Biosynthesis of the erythrocyte anion transport protein. J. Biol. Chem. 256 11337–11344.

    PubMed  CAS  Google Scholar 

  • Brahm, J. (1977) Temperature-dependent changes of chloride transport kinetics in human red blood cells. J. Gen. Physiol. 70 283–306.

    Article  PubMed  CAS  Google Scholar 

  • Brock, C. J. and Tanner, M. J. A. (1986) The human erythrocyte anion-transport protein. Further amino acid sequence from the integral membrane domain homologous with the murine protein. Biochem. J. 235 899–901.

    PubMed  CAS  Google Scholar 

  • Brock, C. J., Tanner, M. J. A., and Kempf, C. (1983) The human erythrocyte anion-transport protein. Partial amino acid sequence, conformation, and a possible molecular mechanism for anion exchange. Biochem. J. 213 577–586.

    PubMed  CAS  Google Scholar 

  • Brown, P. A., Feinstein, M. B., and Sha’afi, R. I. (1975) Membrane proteins related to water transport in human erythrocytes. Nature 254 523–525.

    Article  PubMed  CAS  Google Scholar 

  • Cabantchik, Z. I., Knauf, P. A., and Rothstein, A. (1978) The anion transport system of the red blood cell. The role of membrane protein evaluated by use of “probes”. Biochim. Biophys. Acta 515 239–302.

    PubMed  CAS  Google Scholar 

  • Canfield, V. A. and Macey, R. I. (1984) Anion exchange in human erythrocytes has a large activation volume. Biochim. Biophys. Acta 778 379–384.

    Article  PubMed  CAS  Google Scholar 

  • Cheng, S. and Levy, D. (1980) Characterization of the anion transport system in hepatocyte plasma membranes. J. Biol. Chem. 255 2637–2640.

    PubMed  CAS  Google Scholar 

  • Chiba, T., Sato, Y., and Suzuki, Y. (1986) Amino acid residues complexed with eosin 5-isothiocyanate in band 3 protein of the human erythrocyte. Biochim. Biophys. Acta 858 107–117.

    Article  PubMed  CAS  Google Scholar 

  • Cox, J. V., Moon, R. T., and Lazarides, E. (1985) Anion transporter: highly cell-type-specific expression of distinct polypeptides and transcripts in erythroid and non-erythroid cells. J. Cell. Biol. 100 1548–1557.

    Article  PubMed  CAS  Google Scholar 

  • Craik, J. D., Gouaden, K., and Reithmeier, R. A. F. (1986) Inhibition of phosphate transport in human erythrocytes by 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-C1). Biochim. Biophys. Acta 856 602–609.

    Article  PubMed  CAS  Google Scholar 

  • Cuppoletti, J., Goldinger, J., Kang, B., Jo, I., Berenski, C, and Jung, C. Y. (1985) Anion carrier in the human erythrocyte exists as a dimer. J. Biol. Chem. 260 15714–15717.

    PubMed  CAS  Google Scholar 

  • Dekowski, S. A., Rybicki, A., and Drickamer, K. (1983) A tyrosine kinase associated with the red cell membrane phosphorylates band 3. J. Biol. Chem. 258 2750–2753.

    PubMed  CAS  Google Scholar 

  • Demuth, D. R., Showe, L. C, Ballantine, M., Palumbo, A., Fraser, P. J., Cioe, L., Rovera, G., and Curtis, P. J. (1986) Cloning and structural characterization of a human non-erythroid band 3-like protein. EMBO J. 5 1205–1214.

    PubMed  CAS  Google Scholar 

  • Deuticke, B. (1982) Monocarboxylate transport in erythrocytes. J. Membr. Biol. 70 89–103.

    Article  PubMed  CAS  Google Scholar 

  • Dix, J. A., Verkman, A. S., and Solomon, A. K. (1986) Binding of chloride and a disulfonic stilbene transport inhibitor to red cell band 3. J. Membr. Biol. 89 211–223.

    Article  PubMed  CAS  Google Scholar 

  • Dix, J. A., Ausiello, D. A., Jung, C. Y., and Verkman, A. S. (1985) Target analysis studies of red cell water and urea transport. Biochim. Biophys. Acta 821 243–252.

    Article  PubMed  CAS  Google Scholar 

  • Drenckhahn, D., Schlüter, K., Allen, D. P., and Bennett, V. (1985) Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney. Science 230 1287–1290.

    Article  PubMed  CAS  Google Scholar 

  • Drenckhahn, D., Zinke, K., Schauer, U., Appell, K. C, and Low, P. S. (1984) Identification of immunoreactive forms of human erythrocyte band 3 in nonerythroid cells. Eur. J. Cell Biol. 34 144–150.

    PubMed  CAS  Google Scholar 

  • Drickamer, L. K. (1976) Fragmentation of the 95,000-Dalton transmembrane polypeptide in human erythrocyte membranes. Arrangement of the fragments in the lipid bilayer. J. Biol. Chem. 251 5115–5123.

    PubMed  CAS  Google Scholar 

  • Drickamer, L. K. (1977) Fragmentation of the band 3 polypeptide from human erythrocyte membranes. Identification of regions likely to interact with the lipid bilayer. J. Biol. Chem. 252 6906–6917.

    Google Scholar 

  • DuPre, A. M. and Rothstein, A. (1981) Inhibition of anion transport associated with chymotryptic cleavages of red blood cell band 3 protein. Biochim. Biophys. Acta 646 471–478.

    Article  PubMed  CAS  Google Scholar 

  • Fairbanks, G., Steck, T. L., and Wallach, D. F. H. (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10 2606–2617.

    Article  PubMed  CAS  Google Scholar 

  • Falke, J. J., Kanes, K. J., and Chan, S. I. (1985) The minimal structure containing the band 3 anion transport site. J. Biol. Chem. 260 13294–13303.

    PubMed  CAS  Google Scholar 

  • Frölich, O. (1982) The external anion binding site of the human erythrocyte anion transporter: DNDS binding and competition with chloride. J. Membr. Biol. 56 111–123.

    Google Scholar 

  • Fukuda, M., Dell, A., Oates, J. E., and Fukuda, M. N. (1984) Structure of branched lactoseamino-glycan, the carbohydrate moiety of band 3 isolated from adult human erythrocytes. J. Biol. Chem. 259 8260–8273.

    PubMed  CAS  Google Scholar 

  • Funder, J. and Wieth, J. O. (1976) Chloride transport in human erythroctes and ghosts: a quantitative comparison J. Physiol. 262 679–698.

    PubMed  CAS  Google Scholar 

  • Funder, J., Tosteson, D. C, and Wieth, J. O. (1978) Effects of bicarbonate on lithium transport in human red cells. J. Gen. Physiol. 71 721–746.

    Article  PubMed  CAS  Google Scholar 

  • Garcia, A. M., Kopito, R., and Lodish, H. V. (1987) Expression of band 3 in Xenopus laevis oocytes microinjected with in vitro prepared mRNA. Biophys. J. 51 567a.

    Google Scholar 

  • Grinstein, S., Ship, S., and Rothstein, A. (1978) Anion transport in relation to proteolytic dissection of band 3 protein. Biochim. Biophys. Acta 507 294–304.

    Article  PubMed  CAS  Google Scholar 

  • Gunn, R. B. (1973) A titratable carrier model for monovalent and divalent inorganic anions in red blood cells, in Erythrocytes, Thrombocytes, Leucocytes (Gerlach, E., Moser, K., Deutsch, E., and Wilmanns, W., eds.), Thieme, Stuttgart, pp. 77–79.

    Google Scholar 

  • Hazen-Martin, D. J., Pasternack, G., Spicer, S. S., and Sens, D. A. (1986) Immunolocalization of band 3 protein in normal and cystic fibrosis skin. J. Histochem. Cytochem. 34 823–826.

    Article  PubMed  CAS  Google Scholar 

  • Higashi, T., Richards, C. S., and Uyeda, K. (1979) The interaction of phosphor-fructokinase with erythrocyte membranes. J. Biol. Chem. 254 9542–9550.

    PubMed  CAS  Google Scholar 

  • Hoffmann, E. K. (1986) Anion transport systems in the plasma membrane of vertebrate cells. Biochim. Biophys. Acta 864 1–31.

    PubMed  CAS  Google Scholar 

  • Hsu, L. and Morrison, M. (1983) The interaction of human erythrocyte band 3 with cytoskeletal components. Arch Biochem. Biophys. 227 31–38.

    Article  PubMed  CAS  Google Scholar 

  • Husted, R. F., Cohen, L. H., and Steinmetz, P. R. (1979) Pathways for bicarbonate transfer across the serosal membrane of turtle urinary bladder: studies with a disulfonic stilbene. J. Membr. Biol. 47 27–37.

    Article  PubMed  CAS  Google Scholar 

  • Jay, D. G. (1986) Glycosylation site of band 3, the human erythrocyte anion-exchange protein. Biochemistry 25 554–556.

    Article  PubMed  CAS  Google Scholar 

  • Jay, D. and Cantley, L. (1986) Structural aspects of the red cell anion exchange protein. Ann. Rev. Biochem. 55 511–538.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L. (1976) Proton fluxes associated with erythrocyte membrane anion exchange. J. Membr. Biol. 28 187–205.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L. (1982) Reductive methylation of the two H2DIDS-binding lysine residues of band 3, the human erythrocyte anion transport protein. J. Biol. Chem. 257 7554–7559.

    PubMed  CAS  Google Scholar 

  • Jennings, M. L. (1984) Oligomeric structure and the anion transport function of human erythrocyte band 3 protein. J. Membr. Biol. 80 105–117.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L. and Adams, M. F. (1981) Modification by papain of the structure and function of band 3, the erythrocyte anion transport protein. Biochemistry 20 7118–7122.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L. and Nicknish, J. S. (1984) Erythrocyte band 3 protein. Evidence for multiple membrane-crossing segments in the 17000-dalton chymotryptic fragment. Biochemistry 23 6432–6436.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L. and Nicknish, J. S. (1985) Localization of a site of intermolecular cross-linking in human red blood cell band 3 protein. J. Biol. Chem. 260 5472–5479.

    PubMed  CAS  Google Scholar 

  • Jennings, M. L. and Passow, H. (1979) Anion transport across the erythrocyte membrane, in situ proteolysis of band 3 protein, and cross-linking of proteolytic fragments by 4,4′-diisothiocyano-dihydrostilbene-2,2′-disulfo-nate. Biochim. Biophys. Acta 554 498–519.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L. and Al-Rhaiyel, S. (1988) Modification of a carboxyl group that appears to cross the permeability barrier in the red blood cell anion transporter. J. Gen. Physiol. 92, 161–178.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, M. L., Adams-Lackey, M., and Denney, G. H. (1984) Peptides of human erythrocyte band 3 protein produced by extracellular papain cleavage. J. Biol. Chem. 259 4652–4660.

    PubMed  CAS  Google Scholar 

  • Jennings, M. L., Anderson, M. P., and Monaghan, R. (1986) Monoclonal antibodies against human erythrocyte band 3 protein. J. Biol. Chem. 261 9002–9010.

    PubMed  CAS  Google Scholar 

  • Jennings, M. L. and Anderson, M. P. (1987) Chemical modification and labeling of glutamate residues at the stilbenedisulfonate site of human red blood cell band 3 protein. J. Biol. Chem. 262 1691–1697.

    PubMed  CAS  Google Scholar 

  • Jennings, M. L., Monaghan, R., Douglas, S. M., and Nicknish, J. S. (1985) Functions of extracellular lysine residues in the human erythrocyte anion transport protein. J. Gen. Physiol. 86 653–669,

    Article  PubMed  CAS  Google Scholar 

  • Jentsch, T. J., Stahlknecht, T. R., Hollwede, H., Fischer, D. G., Keller, S. K., and Wiederhold, M. (1985) A bicarbonate-dependent process inhibitable by disulfonic stilbenes and a Na+/H+ exchange mediate 22Na+ uptake into cultured bovine corneal endothelium. J. Biol. Chem. 260, 795–801.

    PubMed  CAS  Google Scholar 

  • Jessen, F., Sjoholm, C, and Hoffman, E. K. (1986) Identification of the anion exchange protein of Ehrlich cells: A kinetic analysis of the inhibitory effects of 4,4′-diisothiocyano-2,2′-stilbene disulfonic acid (DIDS) and labeling of membrane proteins with 3H-DIDS. J. Membr. Biol. 92 195–205.

    Article  PubMed  CAS  Google Scholar 

  • Jones, G. S. and Knauf, P. A. (1985) Mechanism of the increase in cation permeability of human erythrocytes in low-chloride media. Involvement of the anion transport protein capnophorin. J. Gen. Physiol. 86 721–738.

    Article  PubMed  CAS  Google Scholar 

  • Kampmann, L., Lepke, S., Fasold, H., Fritzch, G., and Passow, H. (1982) The kinetics of intramolecular cross-linking of the band 3 protein in the red blood cell membrane by 4,4′-diisothiocyano dihydrostilbene-2,2′disulfonic acid (H2DIDS). J. Membr. Biol. 70 199–216.

    Article  PubMed  CAS  Google Scholar 

  • Kaplan, J. H., Skorah, K., Fasold, H., and Passow, H. (1976) Sidedness of the inhibitory action of disulfonic acids on chloride equilibrium exchange and net transport across the human erythrocyte membrane. FEBS Lett. 62 182–185.

    Article  PubMed  CAS  Google Scholar 

  • Karadsheh, N. S. and Uyeda, K. (1977) Changes in allosteric properties of phosphofructokinase bound to erythrocyte membranes. J. Biol. Chem. 252 7418–7420.

    PubMed  CAS  Google Scholar 

  • Kaul, R. K., Murthy, S. N. P., Reddy, A. G., Stech, T., and Köhler, H. (1983) Amino acid sequence of the N-terminal 201 residues of the human erythrocyte membrane band 3. J. Biol. Chem. 258 7981–7990.

    PubMed  CAS  Google Scholar 

  • Kawano, Y. and Hamasaki, N. (1986) Isolation of a 5,300 dalton peptide containing a pyridoxal phosphate binding site from the 38,000-dalton domain of band 3 of human erythrocyte membranes. J. Biochem. 100 191–199.

    PubMed  CAS  Google Scholar 

  • Kawano, Y., Okubo, K., Tokunaga, F., Miyata, T., Iwanaga, S., and Hamasaki, N. (1988) Localization of the pyridoxal phosphate binding site at the COOH-terminal region of erythrocyte band 3 protein. J. Biol. Chem. 263 8232–8238.

    PubMed  CAS  Google Scholar 

  • Kay, M. M. B. (1984) Localization of senescent cell antigen on band 3. Proc. Natl. Acad. Sci. USA 81 5753–5757.

    Article  PubMed  CAS  Google Scholar 

  • Kay, M. M. B., Tracey, C. M., Goodman, J. R., Cone, J. C, and Bassel, P. A. (1983) Polypeptides immunologically related to band 3 are present in nucleated somatic cells. Proc. Natl. Acad. Sci. USA 80 6882–6886.

    Article  PubMed  CAS  Google Scholar 

  • Kempf, C, Brock, C, Sigrist, H., Tanner, M. J. A., and Zahler, P. (1981) Interaction of phenylisothiocyanate with human erythrocyte band 3 protein. II. Topology of phenylisothiocyanate binding sites and influence of p-sul-fophenylisothiocyanate on phenylisothiocyanate modification. Biochim. Biophys. Acta 641 88–98.

    Article  PubMed  CAS  Google Scholar 

  • Kliman, H. J. and Steck, T. L. (1980) Association of glyceraldehyde-3-phosphate dehydrogenase with the human red cell membrane. A kinetic analysis. J. Biol. Chem. 255 6314–6321.

    PubMed  CAS  Google Scholar 

  • Knauf, P. A. and Rothstein, A. (1971) Chemical modification of membranes. I. Effects of sulfhydryl and amino reactive reagents on anion and cation permeability of the human red blood cell. J. Gen. Physiol. 58 190–210.

    Article  PubMed  CAS  Google Scholar 

  • Knauf, P. A., Law, F. -Y., and Marchant, P. J. (1983) Relationship of net chloride flow across the human erythrocyte membrane to the anion exchange mechanism. J. Gen. Physiol. 81 95–126.

    Article  PubMed  CAS  Google Scholar 

  • Knauf, P. A., Fuhrmann, G. F., Rothstein, S., and Rothstein, A. (1977) The relationship between anion exchange and net anion flow across the human red blood cell membrane. J. Gen. Physiol. 69 363–386.

    Article  PubMed  CAS  Google Scholar 

  • Könne, W., Deuticke, B., and Haest, C. W. M. (1983) Phospholipid dependence of the anion transport system of the human erythrocyte membrane. Biochim. Biophys. Acta 730 139–150.

    Article  Google Scholar 

  • Kopito, R. R. and Lodish, H. F. (1985) Primary structure and transmembrane orientation of the murine anion exchange protein. Nature 316 234–238.

    Article  PubMed  CAS  Google Scholar 

  • Kopito, R. R., Anderson, M. A., and Lodish, F. H. (1987) Multiple tissue-specific sites of transcriptional initiation of the mouse anion transport gene in erythroid and renal cells. Proc. Natl. Acad. Sci. USA 84 7149–7153.

    Article  PubMed  CAS  Google Scholar 

  • Korchak, H. M., Eisenstat, B. A., Hoffstein, S. T., Dunham, P. B., and Weissmann, G. (1980) Anion channel blockers inhibit lysosomal enzyme secretion from human neutrophils without affecting generation of superoxide anion. Proc. Nat. Acad. Sci. USA 77 2721–2725.

    Article  PubMed  CAS  Google Scholar 

  • L’Allemain, G., Paris, S., and Pouysségur, J. (1985) Role of a Na+-dependent Cl-/HCO3 + exchange in regulation of intracellular pH in fibroblasts. J. Biol. Chem. 260 4877–4883.

    PubMed  Google Scholar 

  • Law, F. -Y., Steinfeld, R., and Knauf, P. A. (1983) K562 cell anion exchange differs markedly from that of mature red blood cells. Am J. Physiol. 244 C68-C74.

    PubMed  CAS  Google Scholar 

  • Lepke, S. and Passow, H. (1976) Effects of incorporated trypsin on anion exchange and membrane proteins in human red blood cell ghosts. Biochirn. Biophys. Acta 455 353–370.

    Article  CAS  Google Scholar 

  • Lepke, S. and Passow, H. (1982) Inverse effects of dansylation of red blood cell membrane on band 3 protein-mediated transport of sulphate and chloride. J. Physiol. 328 27–48.

    PubMed  CAS  Google Scholar 

  • Levinson, C. (1978) Chloride and sulfate transport in Ehrlich ascites tumor cells: Evidence for a common mechanism. J. Cell Physiol. 95 23–32.

    Article  PubMed  CAS  Google Scholar 

  • Levinson, C, Corcoran, R. J., and Edwards, E. H. (1979) Interaction of tritium-labeled H2DIDS (4,4′-diisothiocyano-1,2,diphenyl ethane-2,2′disulfonic acid) with the Ehrlich mouse ascites tumor cell. J. Membr. Biol. 45 61–79.

    Article  PubMed  CAS  Google Scholar 

  • Lieberman, D. M., Natriss, M., and Reithmeier, R. A. F. (1987) Carboxypeptidase Y digestion of band 3, the anion transport protein of human erythrocyte membranes. Biochirn. Biophys. Acta 903 37–47.

    Article  CAS  Google Scholar 

  • Low, P. S., Waugh, S. M., Zinke, K., and Drenckhahn, D. (1985) The role of hemoglobin denaturation and band 3 clustering in red blood cell aging. Science 227 531–533.

    Article  PubMed  CAS  Google Scholar 

  • Low, P. S., Westfall, M. A., Allen, D. P., and Appell, K. C. (1984) Characterization of the reversible conformational equilibrium of the cytoplasmic domain of erythrocyte membrane band 3. J. Biol. Chem. 259 13070–13076.

    PubMed  CAS  Google Scholar 

  • Lukacovic, M. F., Verkman, A. S., Dix, J. A., and Solomon, A. K. (1984) Specific interactions of the water transport inhibitor, pCMBS, with band 3 in red blood cell membranes. Biochirn. Biophys. Acta 778 253–259.

    Article  CAS  Google Scholar 

  • Macara, I. G., Kuo, S., and Cantley, L. C. (1983) Evidence that inhibitors of anion exchange induce a transmembrane conformational change in band 3. J. Biol. Chem. 258 1785–1792.

    PubMed  CAS  Google Scholar 

  • Markowitz, S. and Marchesi, V. T. (1981) The carboxyl-terminal domain of human erythrocyte band 3. Description, isolation and location in the bilayer. J. Biol. Chem. 256 6463–6468.

    PubMed  CAS  Google Scholar 

  • Matsuyama, H., Kawano, Y., and Hamasaki, N. (1983) Anion transport activity in the human erythrocyte membrane modulated by proteolytic digestion of the 38,000-dalton fragment in band 3. J. Biol. Chem. 258 15376–15381.

    PubMed  CAS  Google Scholar 

  • Matsuyama, H., Kawano, Y., and Hamasaki, N. (1986) Involvement of a histidine residue in inorganic phosphate and phosphoenolpyruvate transport across the human erythrocyte membrane. J. Biochem. 99 495–501.

    PubMed  CAS  Google Scholar 

  • Mawby, W. J. and Findlay, J. B. C. (1982) Characterization and partial sequence of di-iodosulphophenyl isothiocyanate-binding peptide from human erythrocyte anion-transport protein. Biochem. J. 205 465–475.

    PubMed  CAS  Google Scholar 

  • Milanick, M. A. and Gunn, R. B. (1982) Proton-sulfate co-transport: Mechanism of H+ and sulfate addition to the chloride transporter of human red blood cells. J. Gen. Physiol. 78 547–568.

    Google Scholar 

  • Milanick, M. A. and Gunn, R. B. (1984) Proton-sulfate cotransport: External proton activation of sulfate influx into human red blood cells. Am. J. Physiol. 247 C247-C259.

    PubMed  CAS  Google Scholar 

  • Mühlebach, T. and Cherry, R. J. (1985) Rotational diffusion and self-association of band 3 in reconstituted lipid vesicles. Biochemistry 24 1975–1983.

    Google Scholar 

  • Murthy, P. S. N., Liu, T., Kaul, R. K., Köhler, H., and Steck, T. L. (1981) The aldolase-binding site of the human erythrocyte membrane is at the NH2 terminus of band 3. J. Biol. Chem. 256 11203–11208.

    PubMed  CAS  Google Scholar 

  • Nanri, H., Hamasaki, N., and Minakami, S. (1983) Affinity labeling of erythrocyte band 3 protein with pyridoxal 5-phosphate. J. Biol. Chem. 258 5985–5989.

    PubMed  CAS  Google Scholar 

  • Niggli, V., Sigel, E., and Carafoli, E. (1982) Inhibition of the purified and reconstituted calcium pump of erythrocytes by uM levels of DIDS and NAP-taurine. FEBS Lett. 13 164–166.

    Article  Google Scholar 

  • Oikawa, K., Lieberman, D. M., and Reithmeier, R. A. F. (1985) Conformation and stability of the anion transport protein of human erythrocyte membranes. Biochemistry 24 2843–2848.

    Article  PubMed  CAS  Google Scholar 

  • Olsnes, S., Tønnessen, T. I., and Sandvig, K. (1986) pH-regulated anion antiport in nucleated mammalian cells. J. Cell Biol. 102 967–971.

    Article  PubMed  CAS  Google Scholar 

  • Paradiso, A. M., Tsien, R. Y., and Machen, T. E. (1987) Digital image processing of intracellular pH in gastric oxyntic and chief cells. Nature 325 447–450.

    Article  PubMed  CAS  Google Scholar 

  • Passow, H. (1986) Molecular aspects of band 3 protein-mediated anion transport across the red blood cell membrane. Rev. Physiol. Biochem. Pharmacol. 103 61–203.

    PubMed  CAS  Google Scholar 

  • Pasternack, G. R., Anderson, R. A., Leto, T. L., and Marchesi, V. T. (1985) Interactions between protein 4.1 and band 3. J. Biol. Chem. 260 3676–3683.

    PubMed  CAS  Google Scholar 

  • Raida, M. and Passow, H. (1985) Enhancement of divalent anion transport across the human red blood cell membrane by the water-soluble dansyl chloride derivative 2-(N-piperidine)ethy lamine-l-naphthyl-5-sulfonyl-chloride (PENS-C1). Biochim. Biophys. Acta 812 624–632.

    Article  PubMed  CAS  Google Scholar 

  • Ramjeesingh, M. and Rothstein, A. (1982) The location of a chymotrypsin cleavage site and of other sites in the primary structure of the 17,000-dalton transmembrane segment of band 3, the anion transport protein of red cell. Membr. Biochem. 4 259–269.

    Article  PubMed  CAS  Google Scholar 

  • Ramjeesingh, M., Gaarn, A., and Rothstein, A. (1980) The location of a disulfonic stilbene binding site in band 3, the anion transport protein of the red blood cell membrane. Biochim. Biophys. Acta 599 127–139.

    Article  PubMed  CAS  Google Scholar 

  • Rao, A. (1979) Disposition of the Band 3 polypeptide in the human erythrocyte membrane. The reactive sulfhydryl groups. J. Biol. Chem. 254 3503–3511.

    PubMed  CAS  Google Scholar 

  • Rao, A. and Reithmeier, R. A. F. (1979) Reactive sulfhydryl groups of the Band 3 polypeptide from human erythrocyte membranes. Locations in the primary structure. J. Biol Chem. 254 6144–6150.

    PubMed  CAS  Google Scholar 

  • Rao, A., Martin, P., Reithmeier, R. A. F., and Cantley, L. C. (1979) Location of the stilbene disulfonate binding site of the human erythrocyte anionexchange system by resonance energy transfer. Biochemistry 18 4505–4516.

    Article  PubMed  CAS  Google Scholar 

  • Roos, A. and Boron, W. F. (1981) Intracellular pH. Physiol. Rev. 61 296–434.

    PubMed  CAS  Google Scholar 

  • Rudloff, V., Lepke, S., and Passow, H. (1983) Inhibition of anion transport across the red cell membrane by dinitrophenylation of a specific lysine residue at the H2DIDS binding site of the band 3 protein. FEBS Lett. 163 14–21.

    Article  PubMed  CAS  Google Scholar 

  • Russell, J. M., Boron, W. F., and Brodwick, M. S. (1983) Intracellular pH and Na fluxes in barnacle muscle with evidence for reversal of the ionic mechamism of intracellular pH regulation. J. Gen. Physiol. 82 47–78.

    Article  PubMed  CAS  Google Scholar 

  • Salhany, J. M., Cordes, K. A., and Gaines, E. D. (1980) Light-scattering measurements of hemoglobin binding to the erythrocyte membrane. Evidence for transmembrane effects related to a disulfonic stilbene binding to band 3. Biochemistry 19 1447–1454.

    Article  PubMed  CAS  Google Scholar 

  • Schuster, V. L., Bonsib, S. M., and Jennings, M. L. (1986) Two types of collecting duct mitochondria-rich (intercalated) cells: Lectin and band 3 cytochemistry. Am. J. Physiol. 251 C347-C355.

    PubMed  CAS  Google Scholar 

  • Shami, Y., Rothstein, A., and Knauf, P. A. (1978) Identification of the Cl transport site of human red blood cells by a kinetic analysis of the inhibitory effects of a chemical probe. Biochim. Biophys. Acta 508 357–363.

    Article  PubMed  CAS  Google Scholar 

  • Sigrist, H., Kempf, C., and Zahler, P. (1980) Interaction of phenylisothiocyanate with human erythrocyte Band 3. I. Covalent modification and inhibition of phosphate transport. Biochim. Biophys. Acta 597 137–144.

    Article  PubMed  CAS  Google Scholar 

  • Simchowitz, L. and DeWeer, P. (1986) Chloride movement in human neutrophils. Diffusion, exchange, and active transport. J. Gen. Physiol. 88 195–217.

    Article  PubMed  CAS  Google Scholar 

  • Simchowitz, L. and Roos, A. (1985) Regulation of intracellular pH in human neutrophils. J. Gen. Physiol. 85 443–470.

    Article  PubMed  CAS  Google Scholar 

  • Simchowitz, L., Ratzlaff, R., and DeWeer, P. (1986) Anion/anion exchange in human neutrophils. J. Gen. Physiol. 88 219–236.

    Article  Google Scholar 

  • Simons, T. J. B. (1986) The role of anion transport in the passive movement of lead across the human red cell membrane. J. Physiol. 378 287–312.

    PubMed  CAS  Google Scholar 

  • Solomon, A. K., Chasan, B., Dix, J. A., Lukacovic, M. F., Toon, M. R., and Verkman, A. (1983) The aqueous pore in the red cell membrane: Band 3 as a channel for anions, cations, non-electrolytes, and water. Ann. N.Y. Acad. Sci. 414 97–124.

    Article  PubMed  CAS  Google Scholar 

  • Staros, J. V. (1982) N-hydroxysulfosuccinimide active esters: Bis(N-hydroxy-sulfosuccinimide esters of two dicarboxylic acids are hydrophilic, membrane-impermeant, protein cross-linkers. Biochemistry 21 3950–3955.

    Article  PubMed  CAS  Google Scholar 

  • Staros, J. V. and Kakkad, B. P. (1983) Cross-linking and chymotryptic digestion of the extracytoplasmic domain of the anion exchange channel in intact human erythrocytes. J. Membr. Biol. 74 247–254.

    Article  PubMed  CAS  Google Scholar 

  • Steck, T. L. (1972) Cross-linking the major proteins of the isolated erythrocyte membrane. J. Mol. Biol. 66 295–305.

    Article  PubMed  CAS  Google Scholar 

  • Steck, T. L., Ramos, B., and Strapazon, E. (1976) Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane. Biochemistry 15 1154–1161.

    Article  CAS  Google Scholar 

  • Steck, T. L., Koziarz, J. J., Singh, M. K., Reddy, G., and Kohler, H. (1978) Preparation and analysis of seven major, topographically defined fragments of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane. Biochemistry 17 1216–1222.

    Article  PubMed  CAS  Google Scholar 

  • Stone, D. K., Seldin, D. W., Kokko, J. P., and Jacobson, H. R. (1983) Anion dependence of medullary collecting duct acidification. J. Clin. Invest. 71 1505–1505.

    Article  PubMed  CAS  Google Scholar 

  • Teisinger, J., Zemkova, H., and Vyskocil, F. (1984) The effect of anion channel blockers on enzymatic activity of Na+/K+-ATPase and the electrogenic Na+/K+ pump. FEBS Lett. 175 275–278.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, R.C. (1977) The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. J. Physiol. (Lond.) 273 317–338.

    CAS  Google Scholar 

  • Toon, M. R., Dorogi, P. L., Lukacovic, M. F., and Solomon, A. K. (1985) Binding of DTNB to band 3 in the human red cell membrane. Biochim. Biophys. Acta 818 158–170.

    Article  PubMed  CAS  Google Scholar 

  • Tsai, I. H., Murphy, S. N. P., and Steck, T. L. (1982) Effect of red cell membrane binding on the catalytic activity of glyceraldehyde-3-phosphate dehydrogenase. J. Biol. Chem. 257 1438–1442.

    PubMed  CAS  Google Scholar 

  • Tsuji, T., Irimura, T., and Osawa, T. (1981) The carbohydrate moiety of band 3 glycoprotein of human erythrocyte membranes. J. Biol. Chem. 256 10497–10502.

    PubMed  CAS  Google Scholar 

  • Verkman, A. S., Skorecki, K. L., Jung, C. Y., and Ausiello, D. A. (1986) Target molecular weights for red cell band 3 stilbene and mercurial binding sites. Am. J. Physiol. 251 C541-C548.

    PubMed  CAS  Google Scholar 

  • Walder, J. A., Chatterjee, R., Steck, T. L., Low, P. S., Musso, G. F., Kaiser, E. T., Rogers, P. H., and Arnone, A. (1984) The interaction of hemoglobin with the cytoplasmic domain of band 3 of the human erythrocyte membrane. J. Biol. Chem. 259 10238–10246.

    PubMed  CAS  Google Scholar 

  • Waxman, L. (1979) The phosphorylation of the major proteins of the human erythrocyte membrane. Arch. Biochem. Biophys. 195 300–314.

    Article  PubMed  CAS  Google Scholar 

  • Wieth, J. O. and Bjerrum, P. J. (1982) Titration of transport and modifier sites in the red cell anion transport system. J. Gen. Physiol. 79 253–282.

    Article  PubMed  CAS  Google Scholar 

  • Wieth, J. O. and Bjerrum, P. J. (1983) Transport and modifier sites in capnophorin, the anion transport protein of the erythrocyte membrane, in Structure and Function of Membrane Proteins (Quagliariello, E. and Palmieri, F., eds.), Elsevier Science Pub., Amsterdam, pp. 95–106.

    Google Scholar 

  • Wieth, J. O., Andersen, O. S., Brahm, J., Bjerrum, P. J. and Borders, C. L. (1982) Chloride-bicarbonate exchange in red blood cells: Physiology of transport and chemical modification of binding sites. Phil. Trans. R. Soc. Lond. B 299 383–399.

    Article  CAS  Google Scholar 

  • Williams, D. G., Jenkins, R. E., and Tanner, M. J. A. (1979) Structure of the anion-transport protein of the human erythrocyte membrane. Further studies on the fragments produced by proteolytic digestion. Biochem. J. 181 477–493.

    PubMed  CAS  Google Scholar 

  • Wolpaw, E. W. and Martin, D. L. (1986) A membrane protein in LRM55 glial cells cross-reacts with antibody to the anion exchange carrier of human erythrocytes. Neurosci. Lett. 67 42–47.

    Article  PubMed  CAS  Google Scholar 

  • Yoon, S. C, Toon, M. R., and Solomon, A. K. (1984) Relation between red cell anion exchange and water transport. Biochim. Biophys. Acta 778 385–389.

    Article  PubMed  CAS  Google Scholar 

  • Zoccoli, M. A., Hoopes, R. R., and Karnovsky, M. L. (1982) Rat liver microsomal glucose-6-P translocase. J. Biol. Chem. 257 11296–11300.

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. Michael L. Jennings
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, Indiana University School of Medicine, Evansville, Indiana, USA

    B. U. Raess

  2. Department of Biochemistry, Indiana University School of Medicine, Evansville, Indiana, USA

    Godfrey Tunnicliff

Rights and permissions

Reprints and permissions

Copyright information

© 1989 The Humana Press Inc.

About this chapter

Cite this chapter

Jennings, M.L. (1989). The Anion Transport Protein. In: Raess, B.U., Tunnicliff, G. (eds) The Red Cell Membrane. Contemporary Biomedicine, vol 10. Humana Press. https://doi.org/10.1007/978-1-4612-4500-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-4500-1_8

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-8848-0

  • Online ISBN: 978-1-4612-4500-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation