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In Vivo Effects of Urinary Extracts of Patients with Aplastic Anemia on Rat Platelet Production and Megakaryocyte Progenitors in Murine Spleen and Bone Marrow

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Experimental Hematology Today—1985

Part of the book series: Experimental Hematology Today ((HEMATOLOGY,volume 1985))

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Abstract

We examined the quantitative changes in the circulating platelets of rats and in the megakaryoctye progenitor content of mouse spleens and bone marrows after administration of urinary extracts from patients with aplastic anemia (AA). Single intraperitoneal injections of 140 μg and 30 μg of protein (65–90% ethanol precipitate of crude urinary preparation) were administered to Sprague-Dawley rats and BDF1 mice, respectively. Circulating platelet counts in rats increased significantly 24 h after the injection. Total megakaryocyte colony-forming cells (CFU-Meg) in mouse spleens increased 24 h post-injection, peaked at 48 h, and returned to normal levels 96 h after the injection. Changes in the number of megakaryocyte colonies showed that colonies composed of five or more megakaryocytes increased significantly 6 h after the single injection; however, colonies with two megakaryocytes began to increase significantly 24 h post-injection, peaked by 48 h, and returned to normal levels after 96 h.

A significant increase in the number of solitary megakaryocytes was observed 48 h post-injection, with the maximum number occuring after 72 h, remaining at this relatively high level for up to 96 h after the injection. In contrast to the spleen, no statistically significant changes were observed in either total CFU-Meg or solitary megakaryocytes in the bone marrow during the 96 h of observation after the AA urinary extract injection. Our results show that the urinary extract of aplastic anemia patients contains a factor that directly stimulates splenic megakaryocyte progenitors in vivo as well as the release of platelets from mature megakaryocytes and/or platelet pools.

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References

  1. Nakeff A, Dan i els-MeQueen S (1976) In vitro colony assay for a new class of megakaryocyte precursor: colony-formi ng unit megakaryocyte ( CFU-M ). Proc Soc Exp Biol Med 151: 587–590

    Google Scholar 

  2. Burstein SA, Adamson JW, Thorning D, Harker LA (1979) Characteristics of murine megakaryocytic colonies in vitro. Blood 54: 169–179

    PubMed  CAS  Google Scholar 

  3. Levin J, Levin FC, Metcalf D (1980) The effects of acute thrombocytopenia on megakaryocyte-CFC and granu\ocyte- macrophage-CFC in mice: studies o’f bone marrow and spleen. Blood 56: 274–283

    PubMed  CAS  Google Scholar 

  4. Williams N, Jackson H (1978) Regulation of the proliferati on of murine megakaryocyte progenitor cells by cell cycle. Blood 52: 163 - 170

    PubMed  CAS  Google Scholar 

  5. Penington DG (1979) Megakaryocyte colony culture using a liver cell conditioned medium. Blood Cells 5: 13–23

    PubMed  CAS  Google Scholar 

  6. Odell TT Jr, McDonald TP, Detwiler T (1961) Stimulation of platelet production by serum of platelet-depleted rats. Proc Soc Exp Biol Med 108: 428–431

    PubMed  CAS  Google Scholar 

  7. Harker LA (1970) Regulation of thrombopoiesis. Am J Physiol 218: 1376– 1380

    Google Scholar 

  8. Schreiner DP, Levin J (1970) Detection of thrombopoietic activity in plasma by stimulation of suppressed thrombopoiesis. J Clin Invest 49: 1709– 1713

    Google Scholar 

  9. Cooper GW, Cooper B, Chang C-Y (1970) Demonstration of a circulating factor regulating blood platelet production using S-sulfate in rats and mice. Proc Soc Exp Biol Med 134: 1123–1127

    PubMed  CAS  Google Scholar 

  10. Nakeff A, Roozendaal KJ (1975) Thrombopoietin activity in mice following immune-induced thrombocytopenia. Acta Haematol 54: 340–344

    Article  PubMed  CAS  Google Scholar 

  11. McClure PD, Choi SI (1968) Thrombopoietin and erythropoietin levels in idiopathic thrombocytopenic purpura and iron deficiency anemia. Brit J Haematol 15: 351–354

    Article  CAS  Google Scholar 

  12. Penington DG (1970) Isotope bioassay for “thrombopoietin”. Brit Med J 1: 606–608

    Article  PubMed  CAS  Google Scholar 

  13. McDonald TP (1975) Assay of thrombopoietin utilizing human sera and urine fractions. Biochem Med 13: 101–110

    Article  PubMed  CAS  Google Scholar 

  14. Adams WH, Liu YK, Sullivan LW (1978) Humoral regulation of thrombo- poiesis in man. J Lab Clin Med 91: 141– 147

    Google Scholar 

  15. Schreiner DP, Weinberg J, Enoch D (1980) Plasma thrombopoietic activity in humans with normal and abnormal platelet count. Blood 56: 183-–88

    Google Scholar 

  16. Enomoto K, Kawakita M, Kishimoto S, Katayama N, Miyake T (1980) Thrombopoi esis and megakaryocyte colony stimulating factor in the urine of patients with aplastic anaemia. Brit J Haematol 45: 551–556

    Article  CAS  Google Scholar 

  17. Kawakita M, Enomoto K, Katayama N, Kishimoto S, Miyake T (1981) Thrombo- poiesis- and megakaryocyte colony stimulating factors in the urine of patients with idiopathic thrombocytopenic purpura. Brit J Haematol 48: 609–615

    Article  CAS  Google Scholar 

  18. McDonald TP, Clift R, Lange RD, Nolan C, Tribby IIE, Barlow GH (1975) Thrombopoietin production by human embryonic kidney cells in culture. J Lab Clin Med 85: 59–66

    PubMed  CAS  Google Scholar 

  19. McDonald TP (1980) Effect of thrombopoietin on platelet size of mice. Exp Hematol 8: 527–532

    PubMed  CAS  Google Scholar 

  20. Petursson SR: Discussion following Williams N, Jackson HM, Eger RR, Long MW (1981) The separate roles of factors in murine megakaryocyte colony formation. In: Evatt BL, Levine RF, Williams NT (eds) Megakaryocyte biology an precursors: in vitro cloning and cellular properties. New York: Elsevier/ North-Holland, p74

    Google Scholar 

  21. Levin J, Levin FC, Hull DF III, Penington DG (1982) The effects of thrombopoietin on megakaryocyte-CFC, megakaryocytes and thrombopoiesis: with studies of ploidy and platelet size. Blood 60: 989–998

    PubMed  CAS  Google Scholar 

  22. Freeman MH, McDonald TP, Saunders EF (1981) Differentiation of murine marrow megakaryocyte progenitors (CFUm): humoral control in vitro. Cell Tissue Kinet 14: 53–58

    Google Scholar 

  23. Mazur EM, Hoffman R, Chasis J, Marchesi S, Bruno E (1981) Immunofluor- escent identification of human megakaryocyte colonies using an antiplatelet glycoprotein antiserum. Blood 57: 277– 286

    Google Scholar 

  24. Williams N, Jackson H, Iscove NN, Dukes PP (1984) The role of erythropoietin, thrombopoietic stimulating factor, and myeloid colony stimulating factors on murine megakaryocyte colony formation. Exp Hematol 12: 734–740

    PubMed  CAS  Google Scholar 

  25. Kaneko F, Tajiri M, Matsubara T, Miyake T, Kawakita Y (1971) Urinary erythropoietin. I. Preparation of crude erythropoietin and its biological activity. Kumamoto Med J 24: 95-101

    Google Scholar 

  26. Goldw’asser E, Eliason JF, Sikkema D (1975) Assay for erythropoietin in vitro at the milliunit level. Endocrinology 19: 315–323

    Article  Google Scholar 

  27. Miyake T, Kawakita M, Enomoto K, Murphy MJ Jr (1982) Partial purification and biological properties of thrombopoietin extracted from the urine of aplastic anemia patients. Stem Cells 2: 129–144

    PubMed  CAS  Google Scholar 

  28. Hoffman R, Mazur E, Bruno E, Floyd V (1981) Assay of an activity in the serum of patients with disorders of thrombopoiesis that stimulates formation of megakaryocytic colonies. N Eng J Med 305: 533–538

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Hipple Cancer Research Center and Wright State University, Dayton, Ohio, USA

    Dr. S-I. Kuriya (American Honda Foundation Cancer Research Fellow), Y. Ishida, F. Ali-Osman, C. Mantel & Dr. Martin J. Murphy Jr.

  2. Hipple Cancer Research Center, 4100 South Kettering Boulevard, Dayton, OH, 45439-2092, USA

    Dr. Martin J. Murphy Jr.

Authors
  1. Dr. S-I. Kuriya
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  2. Y. Ishida
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  3. F. Ali-Osman
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  4. C. Mantel
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  5. Dr. Martin J. Murphy Jr.
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Editor information

Editors and Affiliations

  1. Physiology Department, Uniformed Services University of the Health Sciences, 20814, Bethesda, MD, USA

    S. J. Baum

  2. Laboratory of Microbiology and Immunology, National Institute of Dental Research, NIH, 29782, Bethesda, MD, USA

    Dov H. Pluznik

  3. Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    L. A. Rozenszajn

  4. Department of Medical Laboratories, Meir Hospital, Kfar-Sava, Israel

    L. A. Rozenszajn

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© 1986 Springer-Verlag New York Inc.

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Kuriya, SI., Ishida, Y., Ali-Osman, F., Mantel, C., Murphy, M.J. (1986). In Vivo Effects of Urinary Extracts of Patients with Aplastic Anemia on Rat Platelet Production and Megakaryocyte Progenitors in Murine Spleen and Bone Marrow. In: Baum, S.J., Pluznik, D.H., Rozenszajn, L.A. (eds) Experimental Hematology Today—1985. Experimental Hematology Today, vol 1985. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4920-7_6

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  • DOI: https://doi.org/10.1007/978-1-4612-4920-7_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-0-387-96273-3

  • Online ISBN: 978-1-4612-4920-7

  • eBook Packages: Springer Book Archive

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