Micro Quantitative Edman Manual Sequencing

Abstract

The ability to quantitatively as well as qualitatively determine amino acid residues from protein/peptide sequencing would provide an important tool for the study of proteins, which up to recently has not been utilized.¹ Edman clearly noted this; and was surprised so little emphasis was placed on the quantitative aspects of sequencing when so much additional information could be obtained by its realization.² The method to be described attempts to fulfill this intention by having a technical ease which facilitates reproducibility and by incorporating a simple, sensitive, accurate system for quantitation; thus not only protein chemists but anyone dealing with proteins could avail themselves of the potential of quantitative sequencing. In addition the method has the flexibility to effectively treat a broad scope of sequencing problems such as rapidly deblocking and sequencing pyroglutamyl polypeptides and preventing material losses during extractive procedures (without special carriers).