Abstract
The studies described in this paper were directed at an understanding of the regulation of heme synthesis during erythropoietin (epo)-induced erythroid differentiation. Although considerable work has been done on the regulation of heme synthesis in virally—transformed erythroid cell lines which were chemically induced to differentiate along the erythroid pathway1–4 our goal was to study the control of heme formation in normal bone marrow cells, the physiological site of epo action. Our earlier findings showing that heme synthesis is required for the epo-induced globin synthesis in marrow cells5, made this an important problem.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
. Ebert, P.S., Y. Ikawa. 1974. Induction of 6-aminolevulinic acid synthetase during erythroid differentiation of cultured leukemia cells. Proc. Soc. Exp. Biol. Med. 146: 601.
. Sassa, S., S. Granick, C. Chang, A. Kappas. 1975. Induction of enzymes of the heme biosynthetic pathway in Friend leukemia cells in culture, In: Nakao, K., Fisher, I.W., Takaku, F. (eds): Erythropoiesis. Tokyo, University of Tokyo Press. p 383.
. Sassa, S. 1976. Sequential induction of heme pathway enzymes during erythroid differentiation of mouse Friend leukemia virus-infected cells. J. Exp. Med. 143: 305.
. Rutherford, T., G.G. Thompson, M.R. Moore. 1979. Heme bio-synthesis in Friend erythroleukemia cells: Control by ferrochelatase. Proc. Natl. Acad. Sci. USA 76: 833.
. Beru, N., K. Sahr, E. Goldwasser. 1983. Inhibition of heme synthesis in bone marrow cells by succinylacetone: Effect on globin synthesis. J. Cell Bioch. 21: 93.
. Sassa, S., A. Kappas. 1981. Genetic, metabolic and biochemical aspects of the porphyrias, In: Harris H., Hirshhorn, K.H.(eds): Advances in human genetics. New York, Plenum Press, p 121.
. Granick, S., S. Sassa. 1971. 6-Aminolevulinic acid synthetase and the control of heme and chlorophyll synthesis, In Vogel H.J. (ed): Metabolic Regulation, Vol. 5. New York, Academic Press, p 77.
. Woods, J.S., R.L. Dixon. 1972. Studies of the perinatal differences in the activity of hepatic 6-aminolevulinic acid synthetase. Biochem. Pharmacol. 21: 1735.
. Freshney, R.I., J. Paul. 1971. The activities of three enzymes of haem synthesis during hepatic erythropoiesis in the mouse embryo. J. Embryol. Exp. Morphol. 26: 313.
. Hrinda, M.E., E. Goldwasser, 1968. Studies on the control of hemoglobin formation in marrow cells. Ann. N.Y. Acad. Sci. 149: 412.
. Wada, 0., S. Sassa, F. Takaku, Y. Yano, G. Urata, K. Nakao. 1967. Different responses of the hepatic and erythropoietic 6-aminolevulinic acid synthetase of mice. Biochim. Biophys. Acta. 148: 585.
. Yamamoto, M., H. Fujita, N. Watanabe, N. Hayashi, G. Kikuchi. 1986. An immunochemical study of a-aminolevulinate synthese and 6-aminolevulinate dehydratase in liver and erythroid cells of rat. Arch. Bioch. Biophys. 245: 76.
. Nakao, K., S. Sassa, 0. Wada, F. Takaku. 1968. Enzymatic studies on erythroid differentiation and proliferation. Ann. N.Y. Acad. Sci. 149: 224.
. Freshney, R.I., J. Paul, D. Conkie. 1972. Effect of erythropoietin on haemoglobin synthesis and haem synthesizing enzymes of mouse foetal liver cells in culture. J. Embryol Exp. Morphol. 27: 525.
. Sassa, S., A. Urabe. 1979. Uroporphyrinogen I synthase induction in normal human bone marrow cultures: An early and quantitative response of erythroid differentiation. Proc. Natl. Acad. Sci. USA 76: 5321.
. Beru, N., E. Goldwasser. 1985. The regulation of heme biosynthesis during erythropoietin-induced erythroid differentiation. J. Biol. Chem. 260: 9251.
. Bedard, D.L., E. Goldwasser. 1976. On the mechanism of erythropoietin-induced differentiation XV. Induced transcription restricted by cytosine arabinoside. Exp. Cell. Res. 102: 376.
. Gallien-Lartigue 0., E. Goldwasser. 1964. Hemoglobin synthesis in marrow cell culture; the effect of rat plasma on rat cells. Science 145: 277.
. Varticovski, L., J.P. Kushner, B.F. Burnham. 1980. Biosynthesis of porphyrin precursors: Purification and characterization of mammalian L-alanine: a, 6-dioxovaleric acid aminotransferase. J. Biol. Chem. 255: 3742.
. Frydman, R.B., M.L. Tomaro, A. Sburlati, A. Gutnisky. 1986. The regulation of porphobilinogen oxygenase and porphobilinogen deaminase activities in rat bone marrow under conditions of erythropoietic stress. Biochim Biophys Acta. 87Ö: 520.
. Grandchamp, B., H. De Verneuil, C. Beaumont, S. Chretien, 0. Walter, Y. Nordmann. 1987. Tissue specific expression of porphobilinogen deaminase: Two isoenzymes from a single gene. Eur. J. Biochem. 162: 105.
. Chretien, S., A. Dubart, D. Beaupain, N. Raich, B. Grandchamp, J. Rosa, M. Goossens, R. Paul-Henri. 1988. Alternative transcription and splicing of the human porphobilinogen deaminase gene result either in tissue-specific or in housekeeping expression. Proc. Natl. Acad. Sci. USA. 85:6
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Beru, N., Goldwasser, E. (1989). Studies of the Effect of Erythropoietin on Heme Synthesis. In: Ascensao, J.L., Zanjani, E.D., Tavassoli, M., Levine, A.S., MacKintosh, F.R. (eds) Molecular Biology of Erythropoiesis. Advances in Experimental Medicine and Biology, vol 271. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0623-8_10
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0623-8_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-7897-9
Online ISBN: 978-1-4613-0623-8
eBook Packages: Springer Book Archive