Abstract
For a long time it was practically impossible to measure the stoichiometry of GTP hydrolysis during peptide synthesis proceeding on ribosomes, as ribosome preparations usually consist of no more than 10–50% active ribosomes. The other 50–90% of ribosomes in ribosome preparations are incapable of synthesizing peptide in vitro, but can carry out uncoupled with protein synthesis factor-dependent GTP hydrolysis. As yet there are no means of measuring correctly this uncoupled factor-dependent GTP hydrolysis.
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© 1988 Plenum Press, New York
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Gavrilova, L.P., Kakhniashvili, D.G. (1988). GTP Expense at Codon-Specific Translation and During Misreading. In: Zelinka, J., Balan, J. (eds) Metabolism and Enzymology of Nucleic Acids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0749-5_14
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DOI: https://doi.org/10.1007/978-1-4613-0749-5_14
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