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Animal Models for the Study of Fertilization and Early Development in Vitro

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In Vitro Fertilization and Embryo Transfer

Abstract

The development and successful application of human IVF-ET has grown out of pioneering work using animal models, particularly the mouse, rabbit and hamster (Yanagimachi and Chang, 1964; Gwatkin, 1977; Hartmann, 1983; BIggers, 1987). The successful adaptation of these procedures to human gametes and embryos, from the first unequivocal demonstration of IVF (Bavister et al., 1969; Edwards et al., 1969) to the growth of IVF blastocysts in vitro, took only a few years (Edwards, 1980) and the first human IVF birth occurred in 1978 (Steptoe and Edwards, 1978). Since that time, there have been striking improvements in the clinical procedures for human IVF, but the same cannot be said for the laboratory aspects of the technique. Due to a variety of constraints placed upon the use of human embryos, relatively little research can be done in this species. One would expect that relevant new knowledge might come from animal IVF studies. However, although this is true in a limited sense, direct extrapolation from animal studies to the practice of human IVF is not possible. There are two main reasons for this. One is the obvious fact that there are substantial between-species differences in the details of fertilization and early embryonic development. For example, it is well known that mouse embryos do not require exogenous amino acids for growth to the blastocyst stage in vitro, but there is an absolute amino acid requirement for development of rabbit blastocysts (Kane and Foote, 1970).

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Authors
  1. Barry D. Bavister
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Editors and Affiliations

  1. Oregon Regional Primate Research Center, Beaverton, Oregon, USA

    Don P. Wolf

  2. Wisconsin Regional Primate Research Center, Madison, Wisconsin, USA

    Barry D. Bavister

  3. Evanston and Glenbrook Hospitals, Evanston, Illinois, USA

    Marybeth Gerrity

  4. University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Gregory S. Kopf

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© 1988 Plenum Press, New York

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Bavister, B.D. (1988). Animal Models for the Study of Fertilization and Early Development in Vitro. In: Wolf, D.P., Bavister, B.D., Gerrity, M., Kopf, G.S. (eds) In Vitro Fertilization and Embryo Transfer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1005-1_12

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  • DOI: https://doi.org/10.1007/978-1-4613-1005-1_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8288-4

  • Online ISBN: 978-1-4613-1005-1

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