Abstract
Liquid water is essential to the structure and function of living cells and it is thus not surprising that solidification of water by freezing is usually lethal to the cells. Paradoxically, however, appropriate freezing can also preserve cells for long periods of time in a viable state. The challenge to cells during freezing is not their ability to endure storage at very low temperatures, rather it is the lethality of the temperature zone from approximately −15°C to −60°C that the cell must traverse twice: once during cooling and once during warming.
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van Assendelft, O.W., Jones, B.M., Stoddard, L., Kelly, T. (1990). Inexpensive Method for Cryopreservation of Human Peripheral Blood Lymphocytes. In: Smit Sibinga, C.T., Das, P.C., Meryman, H.T. (eds) Cryopreservation and low temperature biology in blood transfusion. Developments in Hematology and Immunology, vol 24. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1515-5_17
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DOI: https://doi.org/10.1007/978-1-4613-1515-5_17
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