Abstract
The density of field populations of cyst nematodes is estimated by counting the numbers of individuals in samples of soil. This raises two questions, 1) how to take a sample and of what size, and 2) how to arrive at a representative count of the individuals in the sample (extraction method, biotest). Here only the first question is discussed. Once the sample is in the laboratory an accurate count can be made of both the cysts and eggs contained therein. Whatever subsampling is done (investigating part of the field sample after thorough mixing, taking aliquots from egg suspensions) is supposed to result in variances according to binomial or Poisson distributions. This stage of perfection may not have been attained in all laboratories (Müller, 1983), but it certainly is not impossible to approach it. An extensive discussion of laboratory methods is to be found in Seinhorst (1986).
This paper is an extensive summary of J. W. Seinhorst (1987). The estimation of densities of nematode populations in field plots and whole fields. II Sampling and sampling error (in preparation).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anscombe, F.J., 1950, Soil sampling for potato root eelworm cysts, Ann. appi Biol., 37: 206.
Fenwick, D.W., 1961, Estimation of Field Populations of Cyst Forming Nematodes of the Genus Heterodera, J. Helminthol., R.T. Leiper Suppl., 63.
Gerard, G. and Berthet, P., 1971, Sampling strategy in censuring patchy populations, in “Statistical Ecology Vol. I: Spatial Patterns and Statistical Distributions”, G.P. Patil, E.C. Pielou and W.E. Waters, eds., University Park and London.
Goodall, P. and Ferris, H., 1980, Plant Parasitic Nematode Distributions in an Alfalfa Field, J. Nematology., 12: 136.
Jones, F.G.W., 1945, Soil populations of beet eelworm (Heterodera schachtii) in relation to cropping, Ann, appl. Biol., 32: 357.
McSorley, M. and Parrado, J.I., 1982, Estimating relative error in nematode numbers from single soil samples composed of multiple cores, J. Nematology., 14: 522.
Müller, J., 1983, Zur Problematik der quantitativen Erfassung von Heterodera schachtii mit Hilfe von Bondenuntersuchungen, I. Ermittlung des Nematodebesatzes in Mischproben; II. Ermittlung des Nematoden- vesatzes in Feldproben; III. Einfluss von Bearbeiter und Extraktionsmethodik, Nachr Bl. dt. PflSchutzdienst (Braunschweig), 35:132; 35:150; 35: 168.
Mugniéry, D. and Zaouchi, H., 1976, Etude critique de la méthode
d’échantillonnage de nematodes a kystes dans le sol et de la validité des différentes critères dTestimation des population, Ann, de Zool. et Ecologie animales, 8:177.
Raeuber, A. and Stelter, H., 1963, Die Sicherheit bei der Bestimmung der Verseuchung eines Feldes mit dem Kartoffelnematoden (Heterodera rostochiensis Woll.), Biol. Zentralbl., 82: 455.
Seinhorst, J.W., 1973, The combined effects of dichloropropane-dichloro- propene mixture injected into the soil and of dazomet applied to the soil surface, Netherl. J. Pl. Path., 79: 194.
Seinhorst, J.W., 1981, The distribution of cysts of Globodera rostochiensis in small plots and the resulting sampling error, Nematologica, 28: 285.
Seinhorst, J.W., 1987, The estimation of densities of nematode populations in field plots and whole fields, I. The extraction of nematodes from soil and plant tissue; II. Sampling and sampling error, (in preparation).
Seinhorst, J.W. and Ouden, H. den, 1980, The effect of different dosages of aldicarb on the multiplication at small population densities of Globodera rostochiensis on potato in rotavated and non rotavated plots, Netherl. J. Pl. Path., 86: 147.
Ouden, H. den, 1957, Betrouwbarrheid van bemonstering, Jaarverslag 1956, Instituut voor Plantenziektenkundig Onderzoek, Wageningen, p. 121.
Widdowson, E., 1962, The estimation of soil populations of Heterodera rostochiensis Woll., in “Progress in Soil Zoology”, P.W. Murphy, ed, London, pp. 59 - 64.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Seinhorst, J.W. (1986). Measuring Field Populations of Cyst Nematodes. In: Lamberti, F., Taylor, C.E. (eds) Cyst Nematodes. Nato ASI Series, vol 121. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2251-1_35
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2251-1_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9318-7
Online ISBN: 978-1-4613-2251-1
eBook Packages: Springer Book Archive