Abstract
One- and two-dimensional gel electrophoresis have been used to examine proteins synthesized by preimplantation mouse embryos and, for each stage analyzed, about 400–600 distinct polypeptides have been resolved (Epstein and Smith, 1974, Van Blerkom and Brockway, 1975, Levinson et al., 1978). In an attempt to characterize further these proteins, we have used concanavalin A (Con A), a plant lectin that binds to mannose residues, to precipitate both total and cell surface glycoproteins synthesized during the preimplantation period. The precipitates were subsequently analyzed by two-dimensional gel electrophoresis and autoradiography.
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References
Epstein, C. J., and Smith, S. A., 1974, Electrophoretic analysis of proteins synthesized by preimplantation mouse embryos. Dev. Biol. 40: 233–244.
Johnson, L. V., and Calarco, P. G., 1980, Mammalian preimplantation development: The cell surface. Anat Rec. 196: 201–219.
Levinson, J., Goodfellow, P., Vadeboncoeur, M., and McDevitt, H., 1978, Identification of stage-specific polypeptides synthesized during murine preimplantation development, Proc. Natl. Acad. Set. USA 75: 3332–3336.
Van Blerkom, J., and Brockway, G. O., 1975, Qualitative patterns of protein synthesis in the preimplantation mouse embryo. I. Normal pregnancy. Dev. Biol. 44: 148–157.
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© 1981 Plenum Press, New York
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Magnuson, T., Epstein, C.J. (1981). Use of Concanavalin A to Monitor Changes in Glycoprotein Synthesis during Early Mouse Development. In: Glasser, S.R., Bullock, D.W. (eds) Cellular and Molecular Aspects of Implantation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3180-3_36
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DOI: https://doi.org/10.1007/978-1-4613-3180-3_36
Publisher Name: Springer, Boston, MA
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