Skip to main content
SpringerLink
Log in

The Human Tumor Cloning Assay: An In Vitro Assay for Antitumor Activity in Solid Tumors

  • Chapter
Cancer Chemotherapy 1

Part of the book series: Cancer Treatment and Research ((CTAR,volume 7))

  • 76 Accesses

Abstract

It is desirable to develop an in vitro system to predict the activity of antineoplastic agents in man, much as bacterial culture and sensitivity techniques are used in the treatment of infectious disease. Such a test would allow the rapid screening of potential anticancer agents for activity and might allow the selection of appropriate chemotherapy for an individual patient’s tumor.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cline M: In Vitro test systems for anticancer drugs. N Engl J Med 280:955, 1969.

    Google Scholar 

  2. Eagle H, Foley GE: Cytotoxicity in human cell cultures as a primary screen for the detection of antitumor agents. Cancer Res 18: 1017–1025, 1958.

    PubMed  CAS  Google Scholar 

  3. Dendy PP, Bozman G, Wheeler TK: In Vitro screening test for malignant human tumors before chemotherapy. Lancet 2: 68–72, 1970.

    Article  Google Scholar 

  4. Lickiss JN, Cane KA, Baikie AG: In Vitro drug selection in antineoplastic chemotherapy. Eur J Cancer 10: 809–814, 1974.

    Article  PubMed  CAS  Google Scholar 

  5. Wright JC, Cobb JP, Gumport SL, Safad D, Walker DG, Golomb FM: Further investigation of the relation between the clinical and tissue culture response to chemotherapeutic agents on human cancer. Cancer 15: 284–293, 1962.

    Article  PubMed  CAS  Google Scholar 

  6. Hurley J, Yount L: Selection of anticancer drug for palliation using tissue culture sensitivity studies. Am J Surgery 109: 39–42, 1965.

    Article  CAS  Google Scholar 

  7. Holmes HL, Little JM: Tissue culture microtest for predicting response of human cancer to chemotherapy. Lancet 2: 985–987, 1974.

    Article  PubMed  CAS  Google Scholar 

  8. Durkin WJ, Ghanta VK, Balch CM, Davis DW, Hiramoto RN: A methodological approach to the prediction of anticancer drug effect in humans. Cancer Res 39: 402–407, 1979.

    PubMed  CAS  Google Scholar 

  9. Weisenthal LM, Marsden J: A novel dye exclusion assay for predicting response to cancer chemotherapy. Proc Am Assoc Cancer Res and ASCO 22: 155, 1981.

    Google Scholar 

  10. DiPaola JA: Analysis of an individual chemotherapy assay system. Natl Cancer Inst Monogr 34: 240–245, 1971.

    Google Scholar 

  11. Kondo T: Prediction of response of tumor and host to cancer chemotherapy. Natl Cancer Inst Monogr 34: 251–256, 1971.

    PubMed  CAS  Google Scholar 

  12. McGuire WL: Hormone receptors: their role in predicting prognosis and response to endocrine therapy. Semin Oncol 5: 428–433, 1978.

    PubMed  CAS  Google Scholar 

  13. Smyth JF, Robins AB, Leese CL: The metabolism of cytosine arabinoside as a predictive test for clinical response to the drug in acute myeloid leukemia. Eur J Cancer 12: 567–573, 1976.

    Article  PubMed  CAS  Google Scholar 

  14. Kessel D: Relevance of In Vitro tests for predicting responsiveness to antitumor agents. Natl Cancer Inst Monogr 34: 138–145, 1971.

    PubMed  CAS  Google Scholar 

  15. Livingston RB: Depression of thymidine labeling index In Vitro predicts effects of chemotherapy in patients. Proc Am Assoc Cancer Res and ASCO 20: 317, 1979.

    Google Scholar 

  16. McDonald GO, Mclver JM, Llamas AJ, Sky–Peck HH: Tumor cytotoxicity: quantitative changes in DNA synthesis as an indicator. Arch Surg 92: 541–547, 1966.

    PubMed  CAS  Google Scholar 

  17. Cline M: Prediction of in vivo cytotoxicity of chemotherapeutic agents by their effect on malignant leukocytes In Vitro. Blood 30: 176–187, 1967.

    PubMed  CAS  Google Scholar 

  18. Roper P, Drewinko B: Comparison of In Vitro methods to determine drug induced cell lethality. Cancer Res 36: 2182–2188, 1976.

    PubMed  CAS  Google Scholar 

  19. Fjelde A: Human tumor cells in tissue culture. Cancer 8: 845–851, 1955.

    Article  PubMed  CAS  Google Scholar 

  20. Leibovitz A, Stinson JC, McCombs WB III, McCoy CE, Mazur KC, Mabry ND: Classification of human colorectal adenocarcinoma cell lines. Cancer Res 36: 4562–4569, 1976.

    PubMed  CAS  Google Scholar 

  21. Pickard RG, Cobb LM, Steel GG: The growth kinetics of xenografts of human colorectal tumors in immune deprived mice. Br J Cancer 31: 36–45, 1975.

    Article  PubMed  CAS  Google Scholar 

  22. Smith IE, Courtenay VD, Gordon MY: A colony forming assay for human tumor xenografts using agar in diffusion chambers. Br J Cancer 34: 476–484, 1976.

    Article  PubMed  CAS  Google Scholar 

  23. Lamerton LF, Steel GG: Growth kinetics of human large bowel cancer growing in immune deprived mice and some chemotherapeutic observations. Cancer 36: 2431–2436, 1975.

    Article  PubMed  CAS  Google Scholar 

  24. Berry RJ, Laing AH, Wells J: Fresh explant culture of human tumors In Vitro and the assessment of sensitivity to cytotoxic chemotherapy. Br J Cancer 31: 218–227, 1975.

    Article  PubMed  CAS  Google Scholar 

  25. Fuskova T, Fuska J, Ivanitskaya LP, Makwkho LV: Different sensitivity to antibiotics of the established intermittent In Vitro - in vivo tumor cell line in comparison with primary culture. Neoplasma 24: 239–242, 1977.

    PubMed  CAS  Google Scholar 

  26. Steel GG. Cell kinetics and cell survival. In: Medical oncology - Medical aspects of malignant disease, Bagshawe KD (ed). Oxford: Blackwell, 1975, pp 49–66.

    Google Scholar 

  27. Pike BL, Robinson WA: Human bone marrow colony growth in agargel. J Cell Physiol 76: 77–81, 1970.

    Article  PubMed  CAS  Google Scholar 

  28. Tepperman AJ, Curtin J, McCulloch EA: Erythropoietic colonies in culture of human marrow. Blood 44: 659–669, 1974.

    PubMed  CAS  Google Scholar 

  29. Hewitt HB, Wilson CW: A survival curve for mammalian leukemic cells irradiated In Vitro. Br J Cancer 13: 69–75, 1959.

    Article  PubMed  CAS  Google Scholar 

  30. Hill RP, Bush RS: A lung colony assay to determine the radiosensitivity of the cells of a solid tumor. Int J Radiat Biol 15: 435–44, 1969.

    Article  CAS  Google Scholar 

  31. Bruce WR, Meeker BE, Valeriote FA: Comparison of the sensitivity of normal and transplanted lymphoma colony forming cells to chemotherapeutic agents administered in vivo. J Natl Cancer Inst 37: 233–45, 1966.

    PubMed  CAS  Google Scholar 

  32. Wilcox WS, Griswold DP, Laster WR, Schabel FM, Skipper HF: Experimental evaluation of potential anticancer agents. XVII. Kinetics of growth and regression after treatment of certain solid tumors. Cancer Chemother Rep 47: 27–39, 1965.

    PubMed  CAS  Google Scholar 

  33. Brown CH, Carbone PP: In Vitro growth of normal and leukemic human bone marrow. J Natl Cancer Inst 46: 989–1000, 1971.

    PubMed  Google Scholar 

  34. Hermens AF, Barendsen GW: Changes of cell proliferation characteristics in a rat rhabdomyosarcoma before and after x-irradiation. Eur J Cancer 5: 173–189, 1969.

    Article  PubMed  CAS  Google Scholar 

  35. Park CH, Bergsagel DE, McCulloch EA: Mouse myeloma tumor stem cells: a primary cell culture assay. J Natl Cancer Inst 46: 411–422, 1971.

    PubMed  CAS  Google Scholar 

  36. Ogawa W, Bergsagel DE, McCulloch EA. Chemotherapy of mouse myeloma: qualitative cell cultures predictive of response in vivo. Blood 41: 7–15, 1973.

    PubMed  CAS  Google Scholar 

  37. McAllister RM, Reed G: Colony growth in agar of cells from neoplastic and nonneoplastic tissues of children. Pediat Res 2: 356–360, 1968.

    Article  PubMed  CAS  Google Scholar 

  38. Altman AJ, Crussi FG, Rierden WJ, Baehner RL: Growth of rhabdomyosarcoma colonies from pleural fluids. Cancer Res 35: 1809–1812, 1975.

    Google Scholar 

  39. Hamburger AW, Salmon SE, Kim MB, Trent JM, Soehnlen BJ, Alberts DS, Schmidt HJ: Direct cloning of human ovarian carcinoma cell in agar. Cancer Res 38: 3438–3444, 1978.

    PubMed  CAS  Google Scholar 

  40. Hamburger AW, Salmon SE: Primary bioassay of human tumor stem cells. Science 197: 461–463, 1977.

    Article  PubMed  CAS  Google Scholar 

  41. Hamburger A, Salmon SE: Primary bioassay of human myeloma stem cells. J Clin Invest 60: 846–854, 1977.

    Article  PubMed  CAS  Google Scholar 

  42. Costachel O, Fadei L, Badea E: Tumor cell suspension culture on non–adhesive substratum. Z Krebsforsch 72: 24–31, 1969.

    Article  PubMed  CAS  Google Scholar 

  43. Salmon SE, Buick RM. Preparation of permanent slides of soft agar colonies of hematopoietic and tumor stem cells. Cancer Res 39: 1133–1136, 1979.

    PubMed  CAS  Google Scholar 

  44. Von Hoff DD, Harris GJ, Johnson G, Glaubiger D: Initial experience with the human tumor stem cell assay system: potentials and problems. In: Human tumor cloning, Salmon SE (ed). New York: Alan Liss, 1980, pp 113–124.

    Google Scholar 

  45. Harris GJ, Zeagler J, Hode KA, Casper J, Von Hoff DD: Ultrastructural analysis of colonies growing in a human tumor cloning system. Cancer (in press).

    Google Scholar 

  46. Trent JM: Cytogenetic analysis of human tumor cells cloned in agar. In: Cloning of human tumor stem cells, Salmon SE (ed). New York: Alan Liss, 1980, pp 165–179.

    Google Scholar 

  47. Buick RN, Stanisic TH, Fry SE, Salmon SE, Trent JM, Krasovich P: Development of an agar/methyl cellulose clonogenic assay for progenitor cells in transitional cell carcinoma of the bladder. Cancer Res 39: 5051–5056, 1979.

    PubMed  CAS  Google Scholar 

  48. Brodeur GM, Sekhon GS, Goldstein MN: Chromosomal aberrations in human neuroblastomas. Cancer 40: 2256–2263, 1977.

    Article  PubMed  CAS  Google Scholar 

  49. Von Hoff DD: New leads from the laboratory for treating testicular cancer. In: Therapeutic progress in ovarian cancer, testicular cancer and sarcomas, Van Oosterom (ed.). The Hague: Martinus Nijhoff, 1980, p225–234.

    Google Scholar 

  50. Leibovitz A: In: Human tumor cells In Vitro, Fogh J (ed). New York: Plenum, 1975, pp 23–50.

    Google Scholar 

  51. Mavligit GM, Hersh EM, McBride CM: Lymphocyte blastogenic responses to autochthonous viable and nonviable human tumor cells. J Natl Cancer Inst 51: 337–343, 1973.

    PubMed  CAS  Google Scholar 

  52. Mattern J, Kaufmann M, Wayss K, Volm M: Studies on the drug sensitivity of short term cultured tumor cell suspensions. In: Human tumors in short term culture, Dendy PP (ed). New York: Academic Press, 1976, pp 301–310.

    Google Scholar 

  53. Pretlow TG, Jones CM, Pretlow TP: Separation of tumor cells by density gradient centrifugation: recent work with human tumors and discussion of the kind of quantitation needed in cell separation experiments. Biophys Chem 5: 99–106, 1976.

    Article  PubMed  CAS  Google Scholar 

  54. Rockwell S: In vivo–In Vitro tumor systems: new models for studying the response of tumors to therapy. Lab Anim Sci 27:831–851, 1977.

    Google Scholar 

  55. Daniels A, Daniels J, Luck EE: Agarose matrix and enzyme disaggregation improve efficiency of the soft agar chemosensitivity test. Proc Am Assoc Cancer Res and ASCO 22: 155, 1981.

    Google Scholar 

  56. VanWezel AL, VanHemert PA, Parisius W, Cucakovich NB, Macmorine HG: Improved cell dispersal method by low temperature incubation of tissue in trypsincitrate solution. Appl Microbiol 24: 506–507, 1972.

    CAS  Google Scholar 

  57. Pretlow TG, Brattain MG, Kreisberg JI: Separation and characterization of epithelial cells from prostates and prostatic carcinoma: a review. Cancer Treat Rep 61: 153–156, 1977.

    PubMed  Google Scholar 

  58. Brattain MG, Pretlow TP, Pretlow TG: Cell fractionation of large bowel cancer. Cancer 40: 2479–2480, 1977.

    Article  PubMed  CAS  Google Scholar 

  59. Kimball PM, Brattain MG, Pitts AM: A soft agar procedure measuring growth of human colonic carcinoma. Br J Cancer 37: 1015–1019, 1978.

    Article  PubMed  CAS  Google Scholar 

  60. Slocum HK, Pavelic ZP, Rustum YM, Creaven PJ, Karakousis C, Takita H, Greco WR: Characterization of cells obtained by medical and enzymatic means from human melanoma and sarcoma and lung tumors. Cancer Res 41: 1428–1434, 1981.

    PubMed  CAS  Google Scholar 

  61. Freshney RI: Tumor cells disaggregated in collagenase. Lancet 2: 488–489, 1972.

    Article  PubMed  CAS  Google Scholar 

  62. Twentyman PR: Sensitivity to 1-3-bis (2-chlorethyl)-1-nitrosourea and l-(2-chloroethyl)-3-(4-methylcyclohexyl)-l-nitrosourea of the EmTG tumor in vivo as determined by both tumor volume and response and In Vitro plating assay. Cancer Res 38: 2395–2400, 1978.

    PubMed  CAS  Google Scholar 

  63. Hodges GM: An overview of tissue culture procedures in tumor biopsy studies. In: Human tumors in short term culture, Dendy PP (ed). New York: Academic Press, 1976, pp 3–14.

    Google Scholar 

  64. Ham RG: Unique requirements of clonal growth. J Natl Cancer Inst 53: 1459–1463, 1974.

    PubMed  CAS  Google Scholar 

  65. Pretlow TG, Weiss EE, Zettergres JG: Problems connected with the separation of different kinds of cells. Int Rev Exp Pathol 14: 91–205, 1975.

    PubMed  Google Scholar 

  66. Von Hoff DD, Casper J, Bradley E, Trent JM, Hodach A, Reichert C, Makuch R, Altman A: Direct cloning of human neuroblastoma cells in soft agar culture. Cancer Res 40:3591– 3597, 1980.

    Google Scholar 

  67. Mattox DE, Von Hoff DD: In Vitro stem cell assay in head and neck squamous carcinoma. Ann Surg 140: 527–530, 1980.

    Article  CAS  Google Scholar 

  68. Von Hoff DD, Weisenthal LM, Ihde DC, Mathews MJ, Layard M, Makuch R: Growth of lung cancer colonies from bronchial washings. Cancer 48: 400–403, 1981.

    Article  Google Scholar 

  69. Pollard EB, Tio F, Whitecar JP, Coltman CA, Jr, Von Hoff DD: Utilization of a soft agar system to monitor for marrow involvement with small cell carcinoma of the lung. Proc Am Assoc Cancer Res and ASCO 21: 192, 1980.

    Google Scholar 

  70. Salmon SE, Hamburger AW, Soehnlen BS, Durie BGM, Alberts DS, Moon TE: Quantitations of differential sensitivity of human tumor stem cells to anticancer drugs. N Engl J Med 298: 1321–1327, 1978.

    Article  PubMed  CAS  Google Scholar 

  71. Salmon SE, Soehnlen BJ, Durie BGM, Alberts DS, Meyskens FL, Chen HSG, Moon TE: Clinical correlation of drug sensitivity in tumor stem cell assay. Proc Am Assoc Cancer Res and ASCO 20: 340, 1979.

    Google Scholar 

  72. Von Hoff DD, Casper J, Bradley E, Sandbach J, Jones D, Makuch R: Association between human tumor colony forming assay results and response of an individual patient’s tumor to chemotherapy. Am J Med 70: 1027–1032, 1981.

    Article  Google Scholar 

  73. Von Hoff DD, Page C, Harris G, Clark G, Cowan J, Coltman CA: Prospective clinical trial of a human tumor cloning system. Proc Am Assoc Cancer Res and ASCO 22: 154, 1981.

    Google Scholar 

  74. Alberts DS, Chen HSG, Young L, Moon TE, Leigh SA, Surwit EA, Salmon SE: Improved survival for relapsing ovarian cancer patients using the human tumor stem cell assay. Proc Am Assoc Cancer Res and ASCO 22: 462, 1981.

    Google Scholar 

  75. Von Hoff DD, Cowan JD, Harris G, Reisdorf G: Human tumor cloning: feasibility and clinical correlations. Cancer Chemother Pharmacol 6: 265–271, 1981.

    Google Scholar 

  76. Blum RH, Carter SK: Adriamycin: a new antitumor drug with significant clinical activity. Ann Intern Med 80: 249–259, 1974.

    PubMed  CAS  Google Scholar 

  77. Salmon SE, Alberts D, Meyskens F, Durie B, Jones S, Soehnler B, Young L: A new concept: In Vitro trial with the human tumor stem cell assay (HTSCA). Proc Am Assoc Cancer Res and ASCO 21: 329, 1980.

    Google Scholar 

  78. Von Hoff DD, Rozencweig M, Muggia FM: Evaluation of cytotoxic drugs. Cancer Treat Rev 6: 1–8, 1979.

    Article  Google Scholar 

  79. Von Hoff DD, Rozencweig M, Soper W, Helman LJ, Davis HL, Penta JS, Muggia FM: Whatever happened to NSC “—”? An analysis of discontinued anticancer agents. Cancer Treat Rep 61: 759–768, 1977.

    Google Scholar 

  80. Von Hoff DD, Gutterman J, Portnoy B, Coltman CA: Human tumor cloning system as a bioassay for interferon. Cancer Chemother and Pharm (in press).

    Google Scholar 

  81. Cowan JD, Von Hoff DD: Use of a human tumor stem cell assay to screen retinoids for antineoplastic activity. 20th Interscience Conference on Antimicrobial Agents and Chemotherapy 20: 29, 1980.

    Google Scholar 

  82. Cowan JD, Coltman CA, Jr, Von Hoff DD: Use of the human tumor cloning system to evaluate cytotoxic activity of misonidazole. Proc Am Assoc Cancer Res and ASCO 22: 233, 1981.

    Google Scholar 

  83. Von Hoff DD, Coltman CA, Jr, Forseth B: Activity of mitoxantrone in a human tumor cloning system: leads for phase II trials in man. Cancer Res 41: 1853–1855, 1981.

    Google Scholar 

  84. Von Hoff DD, Coltman CA, Jr, Forseth B: Activity of 9–10 anthracenedicarboxaldehyde bis (4,5-dihydro-IH-imidazol-2-yl)hydrozone) dihydrochloride (CL 216,942) in a human tumor cloning system: leads for phase II trials in man. Cancer Chemother and Pharm 6: 141–144, 1981.

    Google Scholar 

  85. Kern DH, Campbell MA, Worth GD, Morton DL: Cloning efficiencies and drug sensitivities of freshly prepared suspensions, cryopreserved cells and cell lines derived from human solid tumors. Proc Am Assoc Cancer Res and ASCO 22: 69, 1981.

    Google Scholar 

  86. Tannock I: Cell kinetics and chemotherapy: a critical review. Cancer Treat Rep 62:1117– 1133, 1978.

    Google Scholar 

  87. Silver RT, Young RC, Holland JF: Some new aspects of modern cancer chemotherapy. Am J Med 63: 772–787, 1977.

    Article  PubMed  CAS  Google Scholar 

  88. Valeriote FA, Edelstein MB: The role of cell kinetics in cancer chemotherapy. Semin Oncol 4: 217–226, 1977.

    PubMed  CAS  Google Scholar 

  89. Shackney SE, McCormack GW, Cuchural GJ: Growth rate patterns of solid tumors and their relation to responsiveness to therapy. Ann Int Med 89: 107–121, 1978.

    PubMed  CAS  Google Scholar 

  90. Elson DL, Livingston RB, Coltman CA, Jr, Von Hoff DD: Labeling index depression in human tumor cells cultured in double-layer agar: a potential chemosensitivity assay. Proc Am Assoc Cancer Res and ASCO 21: 164, 1980.

    Google Scholar 

Download references

Authors
  1. John D. Cowan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daniel D. Von Hoff
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. New York University Medical Center, USA

    F. M. Muggia

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Martinus Nijhoff Publishers, The Hague

About this chapter

Cite this chapter

Cowan, J.D., Von Hoff, D.D. (1983). The Human Tumor Cloning Assay: An In Vitro Assay for Antitumor Activity in Solid Tumors. In: Muggia, F.M. (eds) Cancer Chemotherapy 1. Cancer Treatment and Research, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3903-8_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-3903-8_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3905-2

  • Online ISBN: 978-1-4613-3903-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation