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Producing Broth Cultures in Simple Glass Fermentors

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Handbook for Rhizobia

Abstract

A small glass fermentor of 2–3-liter capacity can be used to produce broth cultures to be applied as liquid inoculants in field experiments. It is also suitable for producing starter cultures for medium-sized commercial fermentors. In this chapter, a glass fermentor is assembled and then used for small-scale production of broth cultures. The broth cultures are monitored periodically for quality control, including checks for contamination and the progress of growth and cell multiplication.

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Key Reference

  • Thompson, J A. 1980. Production and quality control of legume inoculants. pp. 489–533. In F.J. Bergersen (ed.) Methods for Evaluating Biological Nitrogen Fixation. John Wiley & Sons, New York.

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© 1994 Springer-Verlag New York, Inc.

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Somasegaran, P., Hoben, H.J. (1994). Producing Broth Cultures in Simple Glass Fermentors. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_25

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  • DOI: https://doi.org/10.1007/978-1-4613-8375-8_25

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4613-8377-2

  • Online ISBN: 978-1-4613-8375-8

  • eBook Packages: Springer Book Archive

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