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In Vivo 13C Magnetic Resonance Spectroscopy and Metabolic Modeling: Methodology

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Neural Metabolism In Vivo

Part of the book series: Advances in Neurobiology ((NEUROBIOL,volume 4))

Abstract

In vivo 13C NMR spectroscopy combined with infusion of 13C-enriched substrates allows non-invasive investigation of brain metabolism in animals and humans. Time courses of 13C label incorporation from the infused substrate(s) into brain metabolites can be analyzed with metabolic models to quantify metabolic rates through specific metabolic pathways such as the neuronal and glial TCA cycles, pyruvate carboxylase, or the glutamate-glutamine cycle. In this chapter, we review the methodology for successful in vivo 13C MRS measurements and metabolic modeling studies. The various aspects of this methodology are described with a focus on challenges specific to in vivo measurements in the brain. References in this review are current as of March 2008.

Authors are listed in alphabetical order except for the first author.

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Notes

  1. 1.

    Unless otherwise stated, thete terms ‘13C MRS’ and ‘13C MR spectra’ in this chapter refer to both direct 13C detection (at 13C frequency) and indirect 1H[13C] detection (at 1H frequency).

  2. 2.

    In principle, the presence of the 13C isotope can alter the rate at which the labeled substrate is metabolized (isotopic effect). In 13C MRS studies, however, it is generally assumed that isotopic effects are negligible, because the presence of 13C atoms does not significantly modify the molecular weight of the substrate.

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Acknowledgements

We thank Dee Koski and Chris Nelson for their excellent technical support. This work was supported by NIH P41 RR008079, P30 NS057091, R01 NS038672 (P.G.H.) and the Keck Foundation. The high-resolution NMR facility at the University of Minnesota is supported with funds from the University of Minnesota Medical School, NSF (BIR-961477) and the Minnesota Medical Foundation.

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  1. Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota Medical School, 2021 6th St SE, Minneapolis, MN, 55455, USA

    Pierre-Gilles Henry Ph.D., Dinesh K. Deelchand Ph.D., Isabelle Iltis Ph.D., Malgorzata Marjanska Ph.D., Gülin Öz Ph.D., Alexander A. Shestov Ph.D. & Julien Valette Ph.D.

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  1. Pierre-Gilles Henry Ph.D.
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  2. Dinesh K. Deelchand Ph.D.
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  3. Isabelle Iltis Ph.D.
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  6. Alexander A. Shestov Ph.D.
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Correspondence to Pierre-Gilles Henry Ph.D. .

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  1. School of Medicine, Hoglund Brain Imaging Center, University of Kansas, Rainbow Blvd. 3901, Kansas City, 66160, Kansas, USA

    In-Young Choi

  2. Inst. Physique de la Matière Complexe, Labo. d'Imagerie Fonctionnelle et, Ecole Polytechnique Fédérale de Lausanne, Lausanne, 1015, Switzerland

    Rolf Gruetter

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Henry, PG. et al. (2012). In Vivo 13C Magnetic Resonance Spectroscopy and Metabolic Modeling: Methodology. In: Choi, IY., Gruetter, R. (eds) Neural Metabolism In Vivo. Advances in Neurobiology, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-1788-0_7

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