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Excitation-Contraction Coupling in Ventricular Myocytes: Effects of Angiotensin II

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Molecular and Subcellular Cardiology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 382))

Abstract

The effects of the vasoactive peptide angiotensin II (AII) on contractility and excitation-contraction coupling in isolated adult rabbit ventricular myocytes were investigated. In most ventricular myocytes, AII (10-8 M) induced a significant increase in fractional shortening which was not associated with an increase in the calcium transient measured with indo-1. AII did increase the intracellular pH by approximately 0.2 5 pH units coincident with the positive inotropic effect. Effects of AII on pH and contractility were blocked by inhibitors of Na+/H+ exchange. AII also increased the rate of pHi recovery from intracellular acidosis at pHi values above 6.9. AII was shown not to affect the L-type inward calcium current. However, in an occasional cell, AII was observed to cause a slight increase in the calcium transient. We hypothesize that this response may reflect an increase of calcium influx on the sodium calcium exchanger, as a consequence of an increase in subsarcolemmal sodium concentration resulting from enhanced Na+-H+ exchange.

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Authors and Affiliations

  1. The Division of Cardiology, University of Utah Medical School, Salt Lake City, UT, 84132, USA

    William H. Barry, Hiroshi Matsui & John H. B. Bridge

  2. The Nora Eccles Harrison Cardiovascular Research Training Institute, University of Utah Medical School, Salt Lake City, UT, 84132, USA

    John H. B. Bridge & Kenneth W. Spitzer

Authors
  1. William H. Barry
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  2. Hiroshi Matsui
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  3. John H. B. Bridge
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  4. Kenneth W. Spitzer
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Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel

    Samuel Sideman (Chairman) & Rafael Beyar (Co-Chairman) (Chairman) &  (Co-Chairman)

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© 1995 Springer Science+Business Media New York

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Barry, W.H., Matsui, H., Bridge, J.H.B., Spitzer, K.W. (1995). Excitation-Contraction Coupling in Ventricular Myocytes: Effects of Angiotensin II. In: Sideman, S., Beyar, R. (eds) Molecular and Subcellular Cardiology. Advances in Experimental Medicine and Biology, vol 382. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1893-8_4

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  • DOI: https://doi.org/10.1007/978-1-4615-1893-8_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5772-8

  • Online ISBN: 978-1-4615-1893-8

  • eBook Packages: Springer Book Archive

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