Abstract
We have studied the neurovirulence for rats of the MAb-resistant variants isolated from a highly neurovirulent JHMV, cl-2. The variants, MM6 and MM13, with point mutation located within the N terminal 100 amino acids (aa) of the SI protein showed no alteration in neurovirulence in comparison with cl-2, showing high neurovirulence. The variants, MM65 and MM85, with a deletion composed of about 150 aa located in the middle of the S1 subunit were revealed to be non-neurovirulent. A variant MM78 with one aa deletion, asparagic acid at number 543 from the N terminus of the S1, was shown to be low-virulence. The neurovirulence of these viruses paralleled with the viral growth potential in the rat brain. However, all of these variants as well as parental cl-2 showed high neurovirulence for mice. These results suggest that the domain composed of about 150 aa in the middle of the Sl is critical for high-neurovirulence of JHMV for rats.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Holmes, K.V., E.W., Doller and J.N. Behnke. Analysis of the function of coronavirus glycoprotein by differential inhibition of synthesis with tunicamycin. Adv. Exp. Med. Biol. 1981;142: 133–142
Spaan, W., D.Cavanagh, and M.C.Horzinek. Coronaviruses: structure and genome expression. J. Gen. Virol. 1988;69:2939–2952.
Dalziel, R.G., P.W.Lampert, P.J.Talbot, and M.J.Buchmeier. Site-specific alteration of murine hepatitis virus type 4 peplomer glycoprotein E2 results in reduced neurovirulence. J.Virol. 1986;59: 463– 471.
Fleming, J.O., M.D.Trousdale, F.A.K.El-Zaatari, S.A.Stohlman, and L.P. Weiner. Pathogenicity of antigenic variants of murine coronavirus JHM selected with monoclonal antibodies. J.Virol. 1986;58: 869–875.
Parker, S.E., T.M. Gallagher, and M.J. Buchmeier. Sequence analysis reveals extensive polymorphism and evidence of deletions within the E2 glycoprotein gene of several strains of murine hepatitis virus. Virology 1989;173:664–673
Wang, F.-L, J.O. Fleming and M.M.C. Lai Sequence analysis of the spike protein gene of murine coronavirus variants: Study of genetic sites affecting neuropathogenicity. Virology 1992; 186: 742–7491.
Taguchi, F., S.G.Siddell, H.Wege, and V.ter Meulen. Characterization of a variant virus selected in rat brain after infection by coronavirus mouse hepatitis virus JHM. J. Virol. 1985;54: 429–435.
Matsubara, Y., R.Watanabe, and F.Taguchi. Neurovirulence of six different murine coronavirus JHMV variants for rats. Virus Res. 1991;20: 45–58.
Kubo, H., S.Y.Takase, and F.Taguchi. Neutralization and fusion inhibition activities of monoclonal antibodies specific for the S1 subunit of the spike protein of neurovirulent murine coronavirus JHMV cl-2 variant. J. Gen. Virol. 1993;74: 1421–1425.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Taguchi, F., Suzuki, H., Takahashi, H., Kubo, H. (1995). Neurovirulence for Rats of the JHMV Variants Escaped from Neutralization with the S1-Specific Monoclonal Antibodies. In: Talbot, P.J., Levy, G.A. (eds) Corona- and Related Viruses. Advances in Experimental Medicine and Biology, vol 380. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1899-0_31
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1899-0_31
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5775-9
Online ISBN: 978-1-4615-1899-0
eBook Packages: Springer Book Archive