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Experiences in Immunoscintigraphy of Leukocytes

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Radiolabeled Blood Elements

Part of the book series: NATO ASI Series ((NSSA,volume 262))

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Abstract

During the selection of monoclonal antibodies (Mab) raised against purified carcinoembryonic antigen (CEA) two Mabs were identified which immunoprecipitated a glycoprotein of 95kD present in perchloric acid extracts of normal lung and on the surface of normal granulocytes. One of these antigens had a molecular weight of 55kD, the other one of 95kD. When tested on frozen sections of colon carcinomas, normal spleen, lung and pancreas each type of Mab gave a clearly different pattern of reactivity. The Mab binding NCA-55 stained granulocytes as well as bronchiolar and alveolar epithelial cells in lung and inter- and intralobular duct apithelial cells in pancreas, whereas the Mab binding on NCA-95 stained only the granulocytes1. NCA-95 is expressed in dependence from the maturation stage of the studied cells2 and is codey by CGM6 gene codes3,4. Although much is known concerning the structure and tissue distribution of these antigens, only recently have researchers reported possible biological roles for NCA and CEA. OIKAWA et al.5 proposed that NCA functions as a homotypic adhesion molecule, a function similar to that suggested for CEA6. His conclusions were based on the observation of an increase in cell-cell aggregation of Chinese hamster ovary cells following transfection of NCA cDNA. LEUSCH et al7 recently described the bacterial binding properities of CEA and provided evidence that NCA has similar properities. The relevance of these findings as it relates to NCA on granulocytes, however, remains to be determined.

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Author information

Authors and Affiliations

  1. Department of Nuclear Medicine, Frankfurt/Main, Germany

    W. Becker & F. Wolf

  2. Max Planck Research Group Rheumatology/Immunology, Frankfurt/Main, Germany

    R. Kinne & F. Emmrich

  3. Department of Medicine III, University of Erlangen-Nuremberg, Frankfurt/Main, Germany

    G. R. Burmester

  4. Radiochemisches Labor, Behring Werke-Höchst AG Frankfurt/Main, Frankfurt/Main, Germany

    A. Schwarz

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  1. W. Becker
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Editor information

Editors and Affiliations

  1. Department of Nuclear Medicine, CSUB Hospital “Princeps d’Espanya”, Barcelona, Spain

    J. Martin-Comin

  2. Division of Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, USA

    M. L. Thakur

  3. Department of Nuclear Medicine, Hospital Clinic, Barcelona, Spain

    C. Piera

  4. Department of Nuclear Medicine, CSUB Hospital “Princeps d’Espanya”, Barcelona, Spain

    M. Roca

  5. Department of Nuclear Medicine, Hospital Clinic, Barcelona, Spain

    F. Lomeña

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Becker, W., Wolf, F., Kinne, R., Emmrich, F., Burmester, G.R., Schwarz, A. (1994). Experiences in Immunoscintigraphy of Leukocytes. In: Martin-Comin, J., Thakur, M.L., Piera, C., Roca, M., Lomeña, F. (eds) Radiolabeled Blood Elements. NATO ASI Series, vol 262. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2462-5_22

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