Abstract
PCR has been used to detect Toxoplasma-DNA in peripheral blood leukocytes (PBL) of patients with suspected Toxoplasmosis. Müller et al (1989) could differentiate three groups of patients with circulating Toxoplasma-Antigen (cTgA) in serum samples:
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1
Patients with positive detection of cTgA in serum samples over a long period, but without detectable Toxoplasma-specific antibody as tested by Immunfluorescence antibody test (IFAT), Direct agglutination (DA), ISAGA, Toxo-IgM-EIA.
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2
Patients with positive detection of cTgA in serum samples for a long period and with low IgG titers (IFAT-IgG 16–256).
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3
Patients with positive detection of cTgA in serum samples and with increased antibody titers (IFAT-IgG >1000, Toxo- IgM-EIA or IFAT-IgM positive).
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References
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© 1994 Springer Science+Business Media New York
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Koch, G., Philipp, E., Handschack, A., Becker, K., Müller, W.A. (1994). Identification of Toxoplasma-DNA by Polymerase Chain Reaction in Peripheral Blood Leukocytes of Patients with Suspected Toxoplasmosis. In: Rolfs, A., Weber-Rolfs, I., Finckh, U. (eds) Methods in DNA Amplification. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2530-1_23
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DOI: https://doi.org/10.1007/978-1-4615-2530-1_23
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